Fourier transform infrared (FT-IR) study on cyanide induced biochemical and structural changes in rat sperm

Shivanoor, Shiddappa Mallappa; David, Muniswamy

doi:10.1016/j.toxrep.2015.10.004

Cited by 22 publications

(19 citation statements)

References 60 publications

(77 reference statements)

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“…We also observed vacuolar degeneration of Sertoli and spermatogenic cells and necrosis of Leydig cells showing deep eosinophilic cytoplasmic staining. This is similar to the results of the study of Shivanoor and David, who showed that exposure to cyanide caused oxidative damage in sperm biomolecules and reproductive organs, resulting in altered sperm function and toxicity to male genital and reproductive organs (Shivanoor & David, 2015).…”

Section: Discussionsupporting

confidence: 91%

Evaluation of the effective dose of amygdalin for the improvement of antioxidant gene expression and suppression of oxidative damage in mice

Albogami

Hassan

Ahmed

et al. 2020

PeerJ

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Background Little is known regarding the toxic and therapeutic doses of amygdalin. Treatment regimens and schedules can vary between humans and animal models, and there have been reports of cyanide toxicity due to amygdalin use. Objective The aim of this study was to evaluate the effect of different doses of amygdalin on antioxidant gene expression and suppression of oxidative damage in mice. Methods Forty adult male mice were divided randomly into four groups (n = 10) as follows and treated orally for two weeks: a control group treated with saline solution, a group treated with amygdalin at 200 mg/kg body weight, a group treated with amygdalin at 100 mg/kg body weight, and a group treated with amygdalin at 50 mg/kg body weight. Liver and testis samples were collected for gene expression, biochemical and histopathological analyses. Results The mice treated with medium-dose amygdalin (100 mg/kg) showed upregulated mRNA expression of glutathione peroxidase (P < 0.01) and superoxide dismutase (P < 0.05) and significantly decreased lipid peroxidation (P < 0.05) in hepatic and testicular tissues compared to those in the untreated groups (controls), with mild histopathological effects. The mice treated with high-dose of amygdalin (200 mg/kg) showed downregulated mRNA expression of glutathione peroxidase and superoxide dismutase (P < 0.01) and significantly increased lipid peroxidation (P < 0.05) in both hepatic and testicular tissues compared to those in the untreated groups (controls), with an apparent effect at the histopathological level. No effects were observed in the mice treated with low-dose amygdalin (50 mg/kg) at the gene, protein and histopathological level. Conclusion Low-and medium-dose amygdalin did not induce toxicity in the hepatic and testicular tissues of male mice, unlike high-dose amygdalin, which had a negative effect on oxidative balance in mice. Therefore, amygdalin at a moderate dose may improve oxidative balance in mice.

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Section: Discussionsupporting

confidence: 91%

Evaluation of the effective dose of amygdalin for the improvement of antioxidant gene expression and suppression of oxidative damage in mice

Albogami

Hassan

Ahmed

et al. 2020

PeerJ

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“… 72 , Raskó and Kiss 71 2269 N=C=O groups (isocyanate) 2272 –O–C≡N groups 3 Júnior et al 73 2199 C≡N stretching (nitrile group) 72 Ruhland et al 74 2144 C–H stretching in alkanes or alkyne C≡C triple bond stretch 128 Coelho et al 75 ; Zhang et al 76 ; Mamunya and Iurzhenko 77 1648 C=O, C=C stretching 1646 C=O, C=C stretching (amide-I, protein C=O stretching) 2 Shashoua et al . 78 , Sławomir 79 1544 C=C stretching or Amide–II (protein N–H bend, C–N stretch) – – – Ivanova and Kolev 81 , Mallappa and Muniswamy 82 1441 CH 2 –bending vibrations of the methyl group; lipids and proteins) 1436 O–CH 3 methyl esters –5 Drasar and Khripach 83 ; Singh et al 57 1277 C–O stretching 1256 PO asymmetric vibrations (phosphate I) –21 Ashtarinezhad et al . 84 , Sławomir 79 1053 PO 4 group vibration …”

Section: Resultsmentioning

confidence: 99%

“…The FTIR peaks located at 1640 and 1540 cm −1 indicate the presence of proteins 80 . In addition to these peaks, one peak at 1544 cm −1 in the spectral range may be attributed to stretching vibration of carboxyl (–CO), C=C stretching or amide-II (C–N stretch, protein N–H bend) groups 23 , 81 , 82 , this peak disappeared in the FTIR spectrum of the Gracilaria biomass sample after biosorption process. The peaks at 1530–1560 cm –1 are attributed to stretching vibration of amino groups (NH stretching) 67 .…”

Section: Resultsmentioning

confidence: 99%

Bioprocessing optimization for efficient simultaneous removal of methylene blue and nickel by Gracilaria seaweed biomass

El‐Naggar

Rabei

2020

Sci Rep

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The pollution of water by heavy metal ions and dyes, particularly from industrial effluents, has become a global environmental issue. Therefore, the treatment of wastewater generated from different industrial wastes is essential to restore environmental quality. The efficiency of Gracilaria seaweed biomass as a sustainable biosorbent for simultaneous bioremoval of Ni2+ and methylene blue from aqueous solution was studied. Optimization of the biosorption process parameters was performed using face-centered central composite design (FCCCD). The highest bioremoval percentages of Ni2+ and methylene blue were 97.53% and 94.86%; respectively, obtained under optimum experimental conditions: 6 g/L Gracilaria biomass, initial pH 8, 20 mg/L of methylene blue, 150 mg/L of Ni2+ and 180 min of contact time. Fourier Transform Infrared Spectroscopy (FTIR) spectra demonstrated the presence of methyl, alkynes, amide, phenolic, carbonyl, nitrile and phosphate groups which are important binding sites involved in Ni2+ and methylene blue biosorption process. SEM analysis reveals the appearance of shiny large particles and layers on the biosorbent surface after biosorption that are absent before the biosorption process. In conclusion, it is demonstrated that the Gracilaria seaweed biomass is a promising, biodegradable, ecofriendly, cost-effective and efficient biosorbent for simultaneous bioremoval of Ni2+ and methylene blue from wastewater effluents.

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“…Cyanide is a potent Complex IV or CIV inhibitor that results in cellular bioenergetic failure from decreased ATP production [ [1] , [2] , [3] , [4] ]. Cyanide exposure can occur from smoke inhalation, especially following the combustion of materials that include wool, silk, synthetic rubber, and polyurethane.…”

Section: Introductionmentioning

confidence: 99%

In vitro comparison of hydroxocobalamin (B12a) and the mitochondrial directed therapy by a succinate prodrug in a cellular model of cyanide poisoning

et al. 2020

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Fourier transform infrared (FT-IR) study on cyanide induced biochemical and structural changes in rat sperm

Cited by 22 publications

References 60 publications

Evaluation of the effective dose of amygdalin for the improvement of antioxidant gene expression and suppression of oxidative damage in mice

Evaluation of the effective dose of amygdalin for the improvement of antioxidant gene expression and suppression of oxidative damage in mice

Bioprocessing optimization for efficient simultaneous removal of methylene blue and nickel by Gracilaria seaweed biomass

In vitro comparison of hydroxocobalamin (B12a) and the mitochondrial directed therapy by a succinate prodrug in a cellular model of cyanide poisoning

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