Fourier Transform Infrared (FTIR) Microscopy and Imaging of Fungi

Naumann, Annette

doi:10.1007/978-3-319-22437-4_4

Cited by 13 publications

(6 citation statements)

References 72 publications

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“…Fourier-transform infrared spectroscopy (FT-IR) involves an infrared light scanning through a sample, which can be a whole cell or its extracts, to generate fingerprint spectra specific to each fungus, since molecules absorb the light in different wavelengths [ 149 ]. The principle of the technique was described by Cooley and Turkey in 1965 [ 150 ].…”

Section: Molecular Diagnosis: Where Do We Stand?mentioning

confidence: 99%

Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives

Pinheiro

Hahn

Camargo

et al. 2020

JoF

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Paracoccidioidomycosis (PCM) is a mycotic disease caused by the Paracoccidioides species, a group of thermally dimorphic fungi that grow in mycelial form at 25 °C and as budding yeasts when cultured at 37 °C or when parasitizing the host tissues. PCM occurs in a large area of Latin America, and the most critical regions of endemicity are in Brazil, Colombia, and Venezuela. The clinical diagnosis of PCM needs to be confirmed through laboratory tests. Although classical laboratory techniques provide valuable information due to the presence of pathognomonic forms of Paracoccidioides spp., nucleic acid-based diagnostics gradually are replacing or complementing culture-based, biochemical, and immunological assays in routine microbiology laboratory practice. Recently, taxonomic changes driven by whole-genomic sequencing of Paracoccidioides have highlighted the need to recognize species boundaries, which could better ascertain Paracoccidioides taxonomy. In this scenario, classical laboratory techniques do not have significant discriminatory power over cryptic agents. On the other hand, several PCR-based methods can detect polymorphisms in Paracoccidioides DNA and thus support species identification. This review is focused on the recent achievements in molecular diagnostics of paracoccidioidomycosis, including the main advantages and pitfalls related to each technique. We discuss these breakthroughs in light of taxonomic changes in the Paracoccidioides genus.

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Section: Molecular Diagnosis: Where Do We Stand?mentioning

confidence: 99%

Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives

Pinheiro

Hahn

Camargo

et al. 2020

JoF

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“…Further, FTIR microscopy has been established for studies of live microorganisms or biomaterials. A detailed assignment of IR bands to their respective molecular bonds and chemical compounds allows for the chemical imaging of fungi, using single hyphae or mycelium pellets, or for chemical imaging of wood cells when studying wood degradation by fungi (Naumann, 2015).…”

Section: Introductionmentioning

confidence: 99%

Essential oils from Thymus spp. as natural biocide against common brown- and white-rot fungi in degradation of wood products: antifungal activity evaluation by in vitro and FTIR analysis

et al. 2023

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Use of wood products in bio-building is increasing because of the raw material´s sustainability and renewability. Degradation of wood by fungi is one of the most signi cant threats in the use of wooden structures. In the past, degradation was prevented by the application of wood preservatives or the use of durable tropical species. Therefore, wood preservation by fossil-based compounds and heavy metals is one of the major issues. This study evaluated the antifungal activity of four essential oils of thyme species (Thymus capitatus, Coridothymus capitatus, T. vulgaris and T. vulgaris Demeter) against two white-rot fungi (Trametes versicolor and Pleurotus ostreatus) and two brown-rot fungi (Poria monticola and Gloeophyllum trabeum). The thyme varietieséssential oils differed in their chemical composition, with either carvacrol, thymol or p-cymene as major components. All four essential oils showed good antifungal activities, in which T. capitatus was the most toxic when applied against P. monticola (MIC of 0.05) and the most e cient inhibiting the growth of the other fungal strains. Fourier transform infrared (FTIR) spectroscopy was applied to investigate fungal cell wall structure under control and stress conditions. The results are a further step towards more eco-friendly solutions for preserving less durable wood products more frequently used in bio-building.

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“…Also, the absorption bands at 924 cm −1 are ascribed to the α- and β-type glycosidic linkages [ 37 ]. The peak around 700–800 and 600 cm −1 is attributed to mannan band and the C–H bending [ 38 ].…”

Section: Resultsmentioning

confidence: 99%

Antioxidant activity and cytotoxicity of exopolysaccharide from mushroom Hericium coralloides in submerged fermentation

Tabibzadeh

Alvandi

Hatamian-Zarmi

et al. 2022

Biomass Conv. Bioref.

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Mushrooms of the genus Hericium spp. represent a series of delicious edible mushrooms with medicinal value. Here, for the first time, the species native to Iran, the mushroom Hericium coralloides , was collected in Mazandaran province, identified, and registered with the NCBI under accession number MW136052. The production of exopolysaccharides (EPS) in submerged culture was optimized using the response surface method. Among the physicochemical and culture medium conditions tested, rotation speed and concentration of maltose and peptone of soybean significantly affected the production of EPS. The proposed model predicts maximum EPS production (0.13 g/L) at 50 g/L maltose, 3 g/L soy peptone, and 1 g/L yeast extract, pH = 6.5, 200 rpm, inoculum at 5% v/v, and 22 °C. The molecular weight of the EPS chains was 413 and 1578 Da. EPS has antioxidant action (EC50 = 6.59 mg/mL) and cytotoxic activity against cancer cells. The viability of AGS and MKN-45 cancer cell lines declined to 20 and 30% after 48 h of the EPS treatment. H. coralloides EPS could be considered a natural dietary anti-cancer supplement. Further studies are necessary to understand the mechanism of the H. coralloides EPS activity on the cell cycle of cancer cells and to prove its action in vivo. Supplementary Information The online version contains supplementary material available at 10.1007/s13399-022-03386-0.

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Fourier Transform Infrared (FTIR) Microscopy and Imaging of Fungi

Cited by 13 publications

References 72 publications

Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives

Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives

Essential oils from Thymus spp. as natural biocide against common brown- and white-rot fungi in degradation of wood products: antifungal activity evaluation by in vitro and FTIR analysis

Antioxidant activity and cytotoxicity of exopolysaccharide from mushroom Hericium coralloides in submerged fermentation

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