Fourier Transform Infrared Quantification of Sugars in Pretreated Biomass Liquors

Tucker, Melvin P.; Mitri, Ragheed K.; Eddy, Fannie P.; Nguyen, Quang; Gedvilas, L. M.; Webb, Jennifer M.

doi:10.1007/978-1-4612-1392-5_2

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…Using FT-MIR spectroscopy, glucose, mannose, xylose, and acetic acid has been rapidly quantified in liquors from dilute-acid-pretreated soft-wood and hard-wood slurries in the batch reactor during optimization of pretreatment conditions (Tucker et al, 2000). …”

Section: Assessing Biotechnological Applications Of Cell Wall Modificmentioning

confidence: 99%

Fourier transform mid infrared spectroscopy applications for monitoring the structural plasticity of plant cell walls

et al. 2014

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Fourier transform mid-infrared (FT-MIR) spectroscopy has been extensively used as a potent, fast and non-destructive procedure for analyzing cell wall architectures, with the capacity to provide abundant information about their polymers, functional groups, and in muro entanglement. In conjunction with multivariate analyses, this method has proved to be a valuable tool for tracking alterations in cell walls. The present review examines recent progress in the use of FT-MIR spectroscopy to monitor cell wall changes occurring in muro as a result of various factors, such as growth and development processes, genetic modifications, exposition or habituation to cellulose biosynthesis inhibitors and responses to other abiotic or biotic stresses, as well as its biotechnological applications.

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Section: Assessing Biotechnological Applications Of Cell Wall Modificmentioning

confidence: 99%

Fourier transform mid infrared spectroscopy applications for monitoring the structural plasticity of plant cell walls

et al. 2014

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“…Therefore, it is critical to rapidly quantify the change in cellulose, hemicelluloses and lignin components of biomass due to application of pre-treatment methods. Infrared spectroscopy has the potential to produce qualitative and quantitative analytical data for samples with minimum or no sample preparation, and at high speed and throughput (Adapa et al, 2011b andBudevska, 2002;Luypaert et al, 2003;Smola and Urleb, 2000;Tucker et al, 2000). Traditionally, chemical analyses of the individual components (e.g., lignin) of lignocellulosics have been performed by acid hydrolysis followed by gravimetric determination of lignin (Kelley et al, 2004).…”

Section: Rapid Characterization Of Lignocellulosic Materialsmentioning

confidence: 99%

Biomass Feedstock Pre-Processing – Part 1: Pre-Treatment

Tabil¹,

Adapa²,

Kashaninejad³

2011

Biofuel's Engineering Process Technology

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“…Rapid techniques for determining the composition of plant cell-walls and the monomeric sugars liberated following enzymatic saccharification include robotics, customized, thermochemically compatible 96-well plates, and modifications of standard laboratory methods [8][9][10][11] and instrumental protocols, such as vibrational spectroscopy (infrared (IR), near-infrared (NIR), or Raman) and nuclear magnetic resonance (NMR) [12][13][14][15][16][17] . These methodologies are key to isolating feedstocks with high cellulose or low lignin contents, or those expected to yield the highest glucose, xylose, ethanol, etc.…”

Section: Introductionmentioning

confidence: 99%

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

Decker

Sykes

Turner

et al. 2015

JoVE

Self Cite

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The conversion of lignocellulosic biomass to fuels, chemicals, and other commodities has been explored as one possible pathway toward reductions in the use of non-renewable energy sources. In order to identify which plants, out of a diverse pool, have the desired chemical traits for downstream applications, attributes, such as cellulose and lignin content, or monomeric sugar release following an enzymatic saccharification, must be compared. The experimental and data analysis protocols of the standard methods of analysis can be time-consuming, thereby limiting the number of samples that can be measured. High-throughput (HTP) methods alleviate the shortcomings of the standard methods, and permit the rapid screening of available samples to isolate those possessing the desired traits. This study illustrates the HTP sugar release and pyrolysis-molecular beam mass spectrometry pipelines employed at the National Renewable Energy Lab. These pipelines have enabled the efficient assessment of thousands of plants while decreasing experimental time and costs through reductions in labor and consumables.

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Fourier Transform Infrared Quantification of Sugars in Pretreated Biomass Liquors

Cited by 3 publications

References 20 publications

Fourier transform mid infrared spectroscopy applications for monitoring the structural plasticity of plant cell walls

Fourier transform mid infrared spectroscopy applications for monitoring the structural plasticity of plant cell walls

Biomass Feedstock Pre-Processing – Part 1: Pre-Treatment

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

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