Fourier-transform infrared spectroscopy: a pharmacotoxicologic tool for in vivo monitoring radical aggression

Abstract

Among the physico-chemical methods that can be used to investigate induced peroxidation in living cells, Fourier transform infrared (FT-IR) spectroscopy appears to be a valuable technique as it is non-destructive and sensitive for monitoring changes in the vibrational spectra of samples. We examined microsomal fractions from rat liver and brain by FT-IR to study the effect of radical aggression induced in vivo by carbon tetrachloride (CCl4). The length of the acyl chains was increased as a consequence of peroxidation induced by the xenobiotic. Moreover, an enhanced level of cholesterol esters and an increase in phospholipids were observed in the liver and the brain, respectively. The conformational structure of the membrane proteins was changed in both the liver and the brain. In the polysaccharide region, we observed an important loss in glucidic structures, such as a decrease in liver glycogen and in some brain glycolipids. These alterations are probably due to the interactions between cells and CCl4 and the metabolic changes caused by CCl4. Thus, FT-IR spectroscopy appears to be an useful tool and an accurate means for rapidly investigating the in vivo biochemical alterations induced by CCl4 in microsomes, and for correlating them with biochemical and physiological data.

Toxicity impact of fenvalerate on the gill tissue of Oreochromis mossambicus with respect to biochemical changes utilizing FTIR and principal component analysis

“…The CH 2 asymmetric stretching band~2927 cm −1 and the symmetric CH 2 stretching band~2849 cm −1 provide information about lipid acyl chain flexibility (an order/disorder of lipids) [26]. This change in intensity and the shift in the frequency asymmetric CH 2 stretching band indicate a change in lipid disorder in the membranes of tissues [13]. In our study, it is observed from Fig.…”

“…The ratio of the intensity of absorption of the bands between I 2960 /I 2873 could be utilized as the principal measure of quantity of methyl groups in protein fibers [12]. Moreover, the ratio of I 2924 /I 2853 can be used to monitor the state of biological membranes [13]. As seen from Fig.…”

Ameliorated Effects of Verapamil on Hepatotoxicity Induced by Ethanol and Carbon Tetrachloride

“…It is also clearly observed that lipid droplets were increased in liver sections treated by CCl 4 and examined by electron microscope. These findings were in parallel to biochemical plasma cholesterol analyses which showed a high significant increase of plasma cholesterol [57]. Verapamil produced the most significant reduction in cholesterol level.…”

A Sensitive Spectrophotometric Method for Determination of Diosmin Using Sodium Nitroprusside as a Chromogenic Reagent

“…A survey of the literature reveled that there are a few methods for diosmin determination in pharmaceutical formulations or biological fluids. These methods include voltammetry, [11] thin-layer chromatography, [12] high performance liquid chromatography (HPLC), [13,14] Fourier transform infrared spectrometry, [15,16] and spectrodensitometry. [17] The methods based on modern instrumental techniques are sensitive, i.e., by the HPLC method, 50 ng diosmin per injection could be detected.…”