Fourier Transform Infrared Spectroscopy of Dysplastic, Papillomavirus-Positive Cervicovaginal Lavage Specimens

Morris, Brian J.; Lee, Claudia; Nightingale, Brian N.; Molodysky, Eugen; Morris, Lilian J.; Appio, Rocco; Sternhell, S.; Cardona, Magnolia; Mackerras, Dorothy; Irwig, Les

doi:10.1006/gyno.1995.1040

Cited by 34 publications

(9 citation statements)

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“…Published studies of exfoliated cervical cells (4)(5)(6)(7)(8) have examined pelleted cells, with each spectrum resulting from all cells and extracellular components in the path of the IR beam. Although it appears intuitive that the spectral abnormalities originate in the abnormal cells, proof of this has never been provided.…”

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confidence: 99%

Cytologically normal cells from neoplastic cervical samples display extensive structural abnormalities on IR spectroscopy: Implications for tumor biology

Cohenford

Rigas

1998

Proc. Natl. Acad. Sci. U.S.A.

146

116

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Fourier-transform IR (FT-IR) spectra of pelleted exfoliated cervical cells from patients with cervical cancer or dysplasia differ from those from normal women. To study the origin of these spectral changes, we obtained the FT-IR spectra of individual cervical cells from normal, dysplastic, and malignant cervical samples. Ninety five percent of normal superficial and intermediate cells displayed two distinct spectral patterns designated A and B, and 5% displayed an intermediate pattern, suggesting extensive structural heterogeneity among these cells. Parabasal and endocervical cells showed pattern B spectra. The spectra of malignant, dysplastic, and other abnormal cells also were characterized. Analysis of FT-IR spectra of over 2,000 individual cells from 10 normal females, 7 females with dysplasia, and 5 females with squamous cell carcinoma revealed that the spectra of normal-appearing intermediate and superficial cells of the cervix from women with either dysplasia or cancer differed from those of normal women. Chemometric and classical spectroscopic analysis showed a continuum of changes paralleling the transition from normalcy to malignancy. These findings suggest that (i) the structural changes underlying the spectroscopic changes are involved in or are a product of cervical carcinogenesis and (ii) the neoplastic process may be more extensive than currently recognized with morphological criteria. This approach may be useful for the structural study of neoplasia and also may be of help in the diagnosis or classification of cervical disorders.Fourier-transform IR spectroscopy (FT-IR) is an emerging method for the study of cancer. We and others have observed that the FT-IR spectra of several human cancers-including colon, skin, and cervical cancer (1-5)-differ from those of their corresponding normal tissues. In cervical cancer, spectral abnormalities have been observed not only in malignant cervical scrapings but also in those with the premalignant condition cervical dysplasia. These findings suggest the occurrence of significant structural, chemical, or metabolic changes associated with cervical carcinogenesis, even at its early stages, and raise the possibility that IR spectroscopy may be useful in the diagnosis of cervical neoplasias.The origin of the spectral changes associated with cervical neoplasia remains unknown. Published studies of exfoliated cervical cells (4-8) have examined pelleted cells, with each spectrum resulting from all cells and extracellular components in the path of the IR beam. Although it appears intuitive that the spectral abnormalities originate in the abnormal cells, proof of this has never been provided. Furthermore, it seems paradoxical that pellets of neoplastic samples (in which the dysplastic or malignant cells are often Ͻ2% of the total) generate grossly abnormal spectra.Resolving these issues requires assessment of the contribution of the individual cellular and noncellular components to the spectra of cervical pellets. To this end, we have developed an approach to o...

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confidence: 99%

Cytologically normal cells from neoplastic cervical samples display extensive structural abnormalities on IR spectroscopy: Implications for tumor biology

Cohenford

Rigas

1998

Proc. Natl. Acad. Sci. U.S.A.

146

116

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“…1 and 2, respectively. The assignment of infrared bands was achieved with the combination of the previously well-established IR spectra of cells and bio-molecules isolated from tissues and cells [5][6][7][8][9] . The peak near 970 cm −1 is generally assigned to the symmetric stretching mode of dianionic phosphate monoester of cellular nucleic acids.…”

Section: Resultsmentioning

confidence: 99%

“…Cervical cancer is a common malignant tumor of the female genital tract. The potential of FTIR as a tool for the screening of cervical cancer has been investigated [5][6][7][8] .…”

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confidence: 99%

Screening cervical lesions with Fourier transform infrared spectroscopy

Gao

et al. 2001

Chin.Sci.Bull.

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The screening results were reported based on the Fourier transform infrared spectroscopy (FTIR) analysis of the samples of exfoliated cervical cells from 354 women. Their spectra can be sorted into two types based on the emerging or not of the absorption bands near 970 cm −1 and 1 170 cm −1 : T1 (83.1%) type without emerging, and T2 (16.9%) type with obviously emerging. All of the samples assigned to T1 were cytologically diagnosed as normal or within normal limits (PapⅠ). 28.9% and 71.1% of samples exhibiting T2 profile, were cytologically evaluated as Pap Ⅰ and abnormal respectively. 3 women in the abnormal group were diagnosed as to have cervical cells with changes associated with high grade of inflammation, cervical scar and cervical erosion. Furthermore, based on the progressive change of the relative intensities of the absorption bands, both T1 and T2 profiles can be categorized into 6 subtypes. The observed heterogeneous spectra and the progressive changes in the absorption frequencies and the relative intensities exhibit features suggestive of the progressive process of cervical lesion. The FTIR method has the potential to complement the cytological smear for large-volume screening of cervical lesions.Keywords: infrared spectral feature, exfoliated cervical cells, screening of cervical lesions.

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“…[41][42][43][44][45][46][47][48] Cervicography allows permanent documentation of the colposcopic findings by use of a cervicograph apparatus, which consists of a camera and strobe light. 49 This technique allows for nonmedical staff to collect the image, which is essentially a ''snap shot'' of the colposcopic appearance.…”

Section: Other Techniques and Technologiesmentioning

confidence: 99%