Fourier transform infrared study of protein secondary structural changes in the muscle of Labeo rohita due to arsenic intoxication

Palaniappan, P. L. R. M.; Vijayasundaram, V.

doi:10.1016/j.fct.2008.09.001

Cited by 46 publications

(42 citation statements)

References 25 publications

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“…So, the sample was employed in 1 mg portions. Each 1 mg portion of sample was thoroughly mixed with 100 mg of completely dried potassium bromide (KBr) (Merck, Darmstadt, Germany; used without further purification), which is a ratio that is frequently used in the pellet technique employed in FTIR spectroscopy (Cakmak et al 2006;Dogan et al 2006;Garip et al 2007;Palaniappan and Vijayasundaram 2008). After mixing with the aid of an agate mortar and pestle, the mixtures were further dried for 1 hr to completely remove any trace of unbound water.…”

Section: Diel Vertical Migration Bioassaymentioning

confidence: 99%

“…Vibrational bands are extremely sensitive to changes in chemical structure, conformation, and their environment (Freifelder 1982;Stuart 1997). Fourier transform infrared (FTIR) Spectroscopy is a powerful tool for the molecular characterization-structurally and functionally-of biomolecules such as lipids, peptides, proteins, nucleic acids, and carbohydrates in a variety of samples including bacteria, plant materials, animal tissues, and foodstuff (Giordano et al 2001;Cakmak et al 2006;Garip et al 2007;Palaniappan and Vijayasundaram 2008). The foremost advantage of FTIR spectroscopy is its ability to present an overall 'organic chemical fingerprint' based on vibrational features of the macromolecular pool of the sample without its destruction.…”

Section: Introductionmentioning

confidence: 99%

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Molecular approach to the chemical characterization of fish-exuded kairomone: a Fourier transform infrared spectroscopic study

et al. 2009

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Diel vertical migration (DVM) bioassay-guided Fourier transform infrared spectroscopy can be a prominent non-destructive and innovative approach for ecological studies. During the characterization of the nature of the fish-exuded kairomone, the peak area results from the spectroscopic analysis of the control, fish-conditioned (F) and temperature incubated fish-conditioned (IF) treatments of the DVM bioassays demonstrated that there was a strong correlation between the alterations of the amine N-H, amide II, amide IV and CH 3 asymmetric vibrations, suggesting that both N-H and CH 3 molecules may be main components of the fish-exuded kairomone cocktail. The IF treatment, which showed similar results with the control treatment, supported that the kairomone is inactivated by bacterial biodegradation. The seasonal variations in the peak areas of the N-H and CH 3 bands suggested that DVM response varied seasonally, where migration response developed quickly in warmer seasons. The peak area of the amine N-H band of the F and IF treatments relative to control conditions can be used as an ideal indicator of the absence or presence and the promptness of migration, at all seasons.

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Section: Diel Vertical Migration Bioassaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular approach to the chemical characterization of fish-exuded kairomone: a Fourier transform infrared spectroscopic study

et al. 2009

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“…Similarly, changes in the intensity of phospodiester band at ~1088 cm -1 showed an increase in the content of nucleic acids of liver but decrease in muscle. Previously a decrease in the nucleic acid content was also observed in the arsenic intoxicated brain tissue of Labeo rohita (Palaniappan and Vijayasundaram 2008). Significant changes in the band areas at 3307, 1543, 1163, 696 of liver tissue and 3364, 2926, 1741, 1653, 1543, 1456, 1394, 1163 of muscles tissue further suggested that Cd had more drastic effect on the tissue architecture as compared to Pb.…”

Section: Discussionmentioning

confidence: 92%

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Khan¹,

Liu²,

Li³

et al. 2017

Turk. J. Fish. Aquat. Sci.

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Contamination of aquatic ecosystems with toxic metals such as Cd 2+ and Pb 2+ is a serious issue in the industrialized world, which can affect freshwater fish even at low concentrations. The aim of the present study was to investigate the effect of Cd 2+ and Pb 2+ alone or in combination on the biochemical constituents of liver and muscle in Crucian carp using Fourier Transform Infrared Spectroscopy (FT-IR). Results from the spectral analysis revealed significant decline in protein and increase in lipids in the two tissues with marked effect caused by the combined exposure of Cd 2+ and Pb 2+ . In case of liver, alteration in the intensity and band area at amide I resulted in a differential response of structural protein for the exposed groups. Furthermore, a decrease in the α-helix and alterations in the nucleic acids content was also observed in both liver and muscle of the exposed fish. Moreover, biochemical alterations in the vital tissue of freshwater fish due to toxic contaminants can be used as a marker of environmental pollution with the help of FT-IR spectroscopy.

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“…The non-specific effects of As consist of a denaturation of proteins caused by hydrogen bond transitions and protein unfolding, involving the Amide I and Amide II bands of proteins [25][26][27][28][29]. As shown in Figure 7, this transition/denaturation is clearly linked to the modification of the β-sheet band at 1632 cm −1 present in the control sample and absent in the exposed sample.…”

Section: Non Specific Structural Changes Detected By Ftir Spectroscopmentioning

confidence: 94%

“…This spectral range has plenty of information about protein structures [24]. By determining the second derivative of the FTIR spectra in the 1700 to 1620 cm −1 sub-range, the changes in the secondary protein structures involving alpha helix, beta sheet, beta turn, and random coil transitions can also be detected [5,14,[25][26][27][28] Moreover, the changes of the ratio between the 1580-1520 cm −1 and the 1700-1580 cm −1 (PO) sub-ranges evidence protein depletion and oxidation [3,14,28,29]. The fatty acid range between 1700 and 1750 cm −1 (already described), when joined to the 1700 to 1620 cm −1 Amide I range of proteins, is also used to determine the lipid to protein ratio (LPR) which is another marker of protein oxidation [20,21].…”

Section: Ftir Spectroscopymentioning

confidence: 99%

Computer Assisted Examination of Infrared and Near Infrared Spectra to Assess Structural and Molecular Changes in Biological Samples Exposed to Pollutants: A Case of Study

Mecozzi

Sturchio

2017

J. Imaging

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Abstract:We present a computer assisted method for the examination of the structural changes present in the probe organism Vicia faba exposed to inorganic arsenic, detected by means of Fourier transform infrared (FTIR) and Fourier transform near infrared (FTNIR) spectroscopy. Like the common ecotoxicological tests, the method is based on the comparison among control and exposed sample spectra of the organisms to detect structural changes caused by pollutants. Using FTIR spectroscopy, we measured and plotted the spectral changes related to the unsaturated to saturated lipid ratio changes (USL), the lipid to protein ratio changes (LPR), fatty and ester fatty acid content changes (FA), protein oxidation (PO) and denaturation, and DNA and RNA changes (DNA-RNA). Using FTNIR spectroscopy, we measured two spectral ranges that belonged to hydrogen bond interactions and aliphatic lipid chains called IntHCONH and Met1overt, respectively. The FTIR results showed that As modified the DNA-RNA ratio and also caused partial protein denaturation in the Vicia faba samples. The FTNIR results supported the FTIR results. The main advantage of the proposed computational method is that it does not require a skilled infrared or near infrared operator, lending support to conventional studies performed by toxicological testing.

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Fourier transform infrared study of protein secondary structural changes in the muscle of Labeo rohita due to arsenic intoxication

Cited by 46 publications

References 25 publications

Molecular approach to the chemical characterization of fish-exuded kairomone: a Fourier transform infrared spectroscopic study

Molecular approach to the chemical characterization of fish-exuded kairomone: a Fourier transform infrared spectroscopic study

Untitled

Computer Assisted Examination of Infrared and Near Infrared Spectra to Assess Structural and Molecular Changes in Biological Samples Exposed to Pollutants: A Case of Study

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