Fourth Interim Report of the AOCS-AOAC Crude Fiber Liaison Committee

Holt, K. E.

doi:10.1093/jaoac/45.3.578

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“…The primary assignment of the eomnfittee was to improve the precision of the method and secondarily to reduce the operating and elapsed times of the procedure. After four years of extensive laboratory investigation and collaborative studies the committee did succeed in making some improvements in the method precision but failed to make any significant changes in the procedure that would reduce the elapsed time required to make a crude fiber determination (3). The basic technique of acid digestion, followed by aIkali digestion, produces an empirical crude fiber result that cannot be reproduced by using any other technique.…”

Section: Introductionmentioning

confidence: 99%

Estimation of crude fiber in dehulled soybeans

Anderson

Holt

1967

J Americ Oil Chem Soc

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The determination of crude fiber in soybeans is a lengthy and time‐consuming procedure which requires extraction, digestion, and incineration and is not suitable as a routine processing‐control tool. A short control procedure has been developed, employing photomicroscopy, which is based on the characteristics of soybean hull cellulose to rotate plane‐polarized light. The sample is mounted on a microscope slide, treated with trichloro‐acetic acid, and placed in a polarizing projection microscope; the image is compared with a series of standard photomicrographs. The amount of hulls present in the sample is measured quantitatively, and an estimation is made on the crude fiber. The crude fiber estimation on de‐hulled soybeans can be made in 15 min compared with 8 to 12 hr by using the official crude fiber method.

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Section: Introductionmentioning

confidence: 99%