FoxA transcription factor Fork head maintains the intestinal stem/progenitor cell identities in Drosophila

Lan, Qing; Cao, Min; Kollipara, Rahul K.; Rosa, Jeffrey B.; Kittler, Ralf; Jiang, Huaqi

doi:10.1016/j.ydbio.2017.09.002

Cited by 18 publications

(15 citation statements)

References 27 publications

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“…Along the same lines, it is important to highlight that the presence of Leishmania in the sand fly midgut leads to the down regulation of genes potentially involved with the control of gene expression. For instance, among the sand fly transcripts down-regulated on day 2 is the transcription factor Forkhead/HNF-3, which is involved with midgut regeneration [22], and nutrient transport and absorption [23]. Accordingly, we have also observed down-regulation of sand fly amino acid and trehalose transporters on 4d after Leishmania infection.…”

Section: Discussionmentioning

confidence: 80%

Leishmania infection induces a limited differential gene expression in the sand fly midgut

Coutinho-Abreu

Serafim

Meneses

et al. 2019

Preprint

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22Background: Phlebotomine sand flies are the vectors of Leishmania worldwide. To develop in 23 the sand fly midgut, Leishmania multiplies and undergoes multiple stage differentiations leading 24 to the infective form, the metacyclic promastigotes. To gain a better understanding of the 25 influence of Leishmania infection in midgut gene expression, we performed RNA-Seq 26 comparing uninfected Lutzomyia longipalpis midguts and Leishmania infantum-infected 27 Lutzomyia longipalpis midguts at seven time points which cover the various developmental 28Leishmania stages including early time points when blood digestion is taking place and late time 29 points when the parasites are undergoing metacyclogenesis. 30Results: Out of over 13,841 transcripts assembled de novo, only 113 sand fly transcripts, about 31 1%, were differentially expressed. Further, we observed a low overlap of differentially expressed 32 sand fly transcripts across different time points suggesting a specific influence of each 33Leishmania stage on midgut gene expression. Two main patterns of sand fly gene expression 34 modulation were noticed. At early time points (days 1-4), more transcripts were down-regulated 35by Leishmania infection at large fold changes (> -32 fold). Among the down-regulated genes, the 36 transcription factor Forkhead/HNF-3 and hormone degradation enzymes were differentially 37 regulated on day 4 and appear to be the upstream regulators of nutrient transport, digestive 38 enzymes, and peritrophic matrix proteins. Conversely, at later time points (days 6 onwards), 39 most of the differentially expressed transcripts were up-regulated by small fold changes (< 32 40 fold), and the molecular function of such genes are associated with the metabolism of lipids and 41 detoxification of xenobiotics (P450). 42 Conclusion:Overall, it appears that Leishmania modulates sand fly gene expression early on in 43 order to overcome the barriers imposed by the midgut, yet it behaves like a commensal at later 44 time points, when modest midgut gene expression changes correlate with a massive amount of 45 parasites in the anterior midgut. 46

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Section: Discussionmentioning

confidence: 80%

Leishmania infection induces a limited differential gene expression in the sand fly midgut

Coutinho-Abreu

Serafim

Meneses

et al. 2019

Preprint

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“…Therefore, Sox21b-1 regulates development of the SAZ, and our observations indicate this is at least in part through roles in organising the germ layers and specification of mesendodermal cells during stages 5 and 6. This is supported by the loss of fkh expression upon Sox21b-1 knockdown, which is required for mesoderm and endoderm formation in both spiders and insects (10, 40, 41). Moreover, the subsequent dynamic expression of Sox21b-1 in the SAZ after stage 6 is suggestive of a role in segment addition.…”

Section: Discussionmentioning

confidence: 91%

A SoxB gene acts as an anterior gap gene and regulates posterior segment addition in the spiderParasteatoda tepidariorum

Paese

Schoenauer

Leite

et al. 2018

Preprint

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SummaryThe Sox gene family encode a set of highly conserved HMG domain transcription factors that regulate many key processes during metazoan embryogenesis. In insects, the SoxB gene Dichaete is the only Sox gene known to be involved in embryonic segmentation. To determine if similar mechanisms are used in other arthropods, we investigated the role of Sox genes during segmentation in the spider Parasteatoda tepidariorum. While Dichaete does not appear to be involved in spider segmentation, RNAi knockdown of the closely related Sox21b-1 gene results in a gap like phenotype in the developing prosoma and also perturbs the sequential addition of opisthosomal segments. We show that this is in part due to a role for Sox21b-1 in regulating the expression of Wnt8 and influencing Delta-Notch signalling during the formation of the segment addition zone. Thus, we have found that two different mechanisms for segmentation in a non-mandibulate arthropod are regulated by a Group B Sox gene. Our work provides new insights into the function of an important and conserved gene family across arthropods, and the evolution of the regulation of segmentation in these animals.

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“…Along the same lines, it is important to highlight that the presence of Leishmania in the sand y midgut leads to the down regulation of genes potentially involved with the control of gene expression. For instance, the transcription factor Forkhead/HNF-3, which is involved with midgut regeneration [23], and nutrient transport and absorption [24], were down-regulated on day 2. Accordingly, we have also observed downregulation of sand y amino acid and trehalose transporters on 4d after Leishmania infection.…”

Section: Discussionmentioning

confidence: 99%

Leishmania infection induces a limited differential gene expression in the sand fly midgut

Coutinho-Abreu

Serafim

Meneses

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Sand flies are the vectors of Leishmania parasites. To develop in the sand fly midgut, Leishmania multiplies and undergoes various stage differentiations giving rise to the infective form, the metacyclic promastigotes. To determine the changes in sand fly midgut gene expression caused by the presence of Leishmania, we performed RNA-Seq of uninfected and Leishmania infantum-infected Lutzomyia longipalpis midguts from seven different libraries corresponding to time points which cover the various Leishmania developmental stages. Results: The combined transcriptomes resulted in the de novo assembly of 13,841 sand fly midgut transcripts. Importantly, only 113 sand fly transcripts, about 1%, were differentially expressed in the presence of Leishmania parasites. Further, we observed distinct differentially expressed sand fly midgut transcripts corresponding to the presence of each of the various Leishmania stages suggesting that each parasite stage influences midgut gene expression in a specific manner. Two main patterns of sand fly gene expression modulation were noted. At early time points (days 1-4), more transcripts were down-regulated by Leishmania infection at large fold changes (> 32 fold). Among the down-regulated genes, the transcription factor Forkhead/HNF-3 and hormone degradation enzymes were differentially regulated on day 2 and appear to be the upstream regulators of nutrient transport, digestive enzymes, and peritrophic matrix proteins. Conversely, at later time points (days 6 onwards), most of the differentially expressed transcripts were up-regulated by Leishmania infection with small fold changes (< 32 fold). The molecular functions of these genes have been associated with the metabolism of lipids and detoxification of xenobiotics. Conclusion: Overall, our data suggest that the presence of Leishmania produces a limited change in the midgut transcript expression profile in sand flies. Further, Leishmania modulates sand fly gene expression early on in the developmental cycle in order to overcome the barriers imposed by the midgut, yet it behaves like a commensal at later time points where a massive number of parasites in the anterior midgut results only in modest changes in midgut gene expression.

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FoxA transcription factor Fork head maintains the intestinal stem/progenitor cell identities in Drosophila

Cited by 18 publications

References 27 publications

Leishmania infection induces a limited differential gene expression in the sand fly midgut

Leishmania infection induces a limited differential gene expression in the sand fly midgut

A SoxB gene acts as an anterior gap gene and regulates posterior segment addition in the spiderParasteatoda tepidariorum

Leishmania infection induces a limited differential gene expression in the sand fly midgut

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