FOXA transcriptional factor modulates insect susceptibility to Bacillus thuringiensis Cry1Ac toxin by regulating the expression of toxin-receptor ABCC2 and ABCC3 genes

Li, Jianghuai; Ma, Ye; Yuan, Wanli; Xiao, Yutao; Liu, Chenxi; Jia, Wang; Peng, Jianxin; Peng, Rong; Soberón, Mário; Bravo, Alejandra; Yang, Yong; Liu, Kaiyu

doi:10.1016/j.ibmb.2017.07.004

Cited by 38 publications

(30 citation statements)

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“…Recent reports revealed the induced expression of CYP6DA2 by gossypol in Aphis gossypii was regulated by AhR (Peng et al ., ) and CncC (Peng et al ., ). Forkhead box A transcriptional factor modulates insect susceptibility to Bacillus thuringiensis Cry1Ac toxin by regulating the expression of toxin‐receptor ABC‐C2 and ABC‐C3 genes (Li et al ., ). Meanwhile, Li et al .…”

Section: Discussionmentioning

confidence: 97%

The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides

et al. 2017

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Cytochrome P450 and UDP-glucosyltransferase (UGT) as phase I and phase II metabolism enzymes, respectively, play vital roles in the breakdown of endobiotics and xenobiotics. Insects can increase the expression of detoxification enzymes to cope with the stress from xenobiotics including insecticides. However, the molecular mechanisms for insecticide detoxification in Spodoptera exigua remain elusive, and the genes conferring insecticide metabolisms in this species are less well reported. In this study, 68 P450 and 32 UGT genes were identified. Phylogenetic analysis showed gene expansions in CYP3 and CYP4 clans of P450 genes and UGT33 family of this pest. P450 and UGT genes exhibited specific tissue expression patterns. Insecticide treatments in fat body cells of S. exigua revealed that the expression levels of P450 and UGT genes were significantly influenced by challenges of abamectin, lambda-cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb. Multiple genes for detoxification were affected in expression levels after insecticide exposures. The results demonstrated that lambda-cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb induced similar responses in the expression of P450 and UGT genes in fat body cells; eight P450 genes and four UGT genes were co-up-regulated significantly, and no or only a few CYP/UGT genes were down-regulated significantly by these four insecticides. However, abamectin triggered a distinct response for P450 and UGT gene expression; more P450 and UGT genes were down-regulated by abamectin than by the other four compounds. In conclusion, P450 and UGT genes from S. exigua were identified, and different responses to abamectin suggest a different mechanism for insecticide detoxification.

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Section: Discussionmentioning

confidence: 97%

The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides

et al. 2017

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“…These data suggest that the heat-shock induction of SfHsp genes may depend not only on temperature and number of HSEs, but also on other factors yet to be identified. Promoters of ie1 and ie2 are most commonly used in transient gene expression studies in insect cells [46][47][48][49][50] . However, lower expression of foreign proteins in insect cells was observed when using ie1/ie2-based transient gene expression systems [51][52][53] .…”

Section: Discussionmentioning

confidence: 99%

Identification and functional analysis of promoters of heat-shock genes from the fall armyworm, Spodoptera frugiperda

Chen

Tan

Palli

2020

Sci Rep

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The functional information on heat-shock proteins (Hsp) and heat-shock promoters from an important agricultural insect pest, Spodoptera frugiperda, is still lacking. We conducted a genomewide identification of Hsp genes and identified a total of 21 genes belonging to four major insect Hsp families (small heat-shock proteins, Hsp60, Hsp70, and Hsp90) in S. frugiperda. Expression of most of S. frugiperda (SfHsp) genes could be detected in Sf9 cells, embryos and larval tissues of S. frugiperda. The heat-inducible activity of heat-shock promoters from several SfHsp genes was tested in Sf9 cells and embryos. The promoter of SfHsp70D showed the high constitutive activity in cell line and embryos, while the activity of SfHsp20.15 and SfHsp20.71 promoters was most dramatically induced in Sf9 cells and embryos. In embryos, the heat-induced activity of SfHsp20.71 and SfHsp70D promoters outperformed commercially used ie1 and ie2 promoters. The heat-induced activity of SfHsp70D and SfHsp19.07 promoters were more robust than ie2 promoter in Sf9 cells. These SfHsp promoters with high basal activity or with heat-induced activity from low basal activity, could be used in S. frugiperda or other lepidopteran insects for many applications including transgenesis and genome editing.Heat-shock proteins (Hsp) are abundant and ubiquitously expressed in insects playing important roles in enhancing abiotic and biotic stress tolerance, as well as regulating normal development 1,2 . Based on their molecular mass and function, insect Hsp can be divided into four major families, small heat-shock proteins, Hsp60, Hsp70, and Hsp 90 2 . Expression of Hsp from a wide range of insect species have been reported to be induced and modulated by abiotic stressors, including extreme temperature 3-5 , ultraviolet radiation 6,7 , pesticides 8,9 , heavy metals 10,11 , desiccation 12-14 , starvation 15,16 , and anoxia/hypoxia 17-19 , as well as several biotic insults, including parasites 20,21 , pathogens 22,23 , and high population density 24 . In recent years, the availability of both transcriptome and genome data greatly contributed to the identification of an increasing number of Hsp genes from diverse insect species and promoted their functional studies 5,9,[25][26][27] . However, information of Hsp genes from a destructive insect pest, Spodoptera frugiperda, is still limited 23,28 .The stress-inducible expression of Hsp gene is conferred by binding of heat-shock factor (HSF) to heat-shock elements (HSEs), which consists of arrays of the 5-bp unit NGAAN arranged as inverted repeats in the promoter region 29 . Since exposure to high temperature is likely the simpler way to achieve inducible expression of insect Hsp genes, the promoters of insect Hsp genes are good candidates to drive the expression of foreign genes by heat-shock. Analysis of heat-shock promoters is still limited to a few model insect species. Promoters of Hsp26, Hsp68, Hsp70, and Hsp82, from Drosophila melanogaster have been used to drive the heat-shock induced expression of transposase...

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“…Previous work has shown that overproduction of detoxification enzymes is an important mechanism for insecticide resistance and complex regulatory mechanisms are involved in the up‐regulated expression of resistance‐associated detoxification genes, such as cis ‐regulatory elements and/or trans ‐acting factors, microRNAs and GPCR signaling cascades . The up‐regulation of CYP6FU1 conferring deltamethrin resistance in Laodelphax striatellus involves multiple cis ‐regulatory elements .…”

Section: Discussionmentioning

confidence: 99%

Transcription factors CncC/Maf and AhR/ARNT coordinately regulate the expression of multiple GSTs conferring resistance to chlorpyrifos and cypermethrin inSpodoptera exigua

Huang

et al. 2019

Pest Management Science

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BACKGROUND: Glutathione S-transferases (GSTs) are a superfamily of multifunctional dimeric proteins existing in both prokaryotic and eukaryotic organisms. They are involved in the detoxification of both endogenous and exogenous electrophiles, including insecticides. However, the molecular mechanisms underlying the regulation of GST genes in insects are poorly understood. RESULTS:We first identified at least three GST genes involved in resistance to the insecticides chlorpyrifos and cypermethrin. Analysis of upstream sequences revealed that three GSTs (SeGSTo2, SeGSTe6 and SeGSTd3) harbor the same cap 'n' collar C/muscle aponeurosis fibromatosis (CncC/Maf) binding site, and SeGSTo2 and SeGSTe6 contain the aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator (AhR/ARNT) binding site. Luciferase reporter assay showed co-transfection of reporter plasmid containing the SeGSTe6 promoter with CncC and/or Maf expressing constructs significantly boosted transcription. Similarly, AhR and/or ARNT expressing constructs also significantly increased the promoter activities. The co-transfection of mutated reporter plasmid with CncC/Maf or AhR/ARNT did not increase transcription activity anymore. Constitutive over-expression of CncC, Maf and AhR was also found in the HZ16 strain, which might be the molecular mechanism for up-regulated expression of multiple detoxification genes conferring resistance to insecticides. CONCLUSION: These results suggest that CncC/Maf and AhR/ARNT coordinately regulate the expression of multiple GST genes involved in insecticide resistance in Spodoptera exigua.

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FOXA transcriptional factor modulates insect susceptibility to Bacillus thuringiensis Cry1Ac toxin by regulating the expression of toxin-receptor ABCC2 and ABCC3 genes

Cited by 38 publications

References 60 publications

The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides

The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides

Identification and functional analysis of promoters of heat-shock genes from the fall armyworm, Spodoptera frugiperda

Transcription factors CncC/Maf and AhR/ARNT coordinately regulate the expression of multiple GSTs conferring resistance to chlorpyrifos and cypermethrin inSpodoptera exigua

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