Foxp3⁺Helios⁺regulatory T cells are expanded in active systemic lupus erythematosus

Abstract

Our data suggest that Helios-expressing Foxp3(+) Treg with functional suppressive capacity and migratory potential into inflamed tissues are expanded in active SLE, presumably through γ-chain signalling cytokines and TCR stimulation, to compensate for autoreactive effector responses.

Effects of aging on human leukocytes (part II): immunophenotyping of adaptive immune B and T cell subsets

“…We observed a strong decrease in + cells in the elderly, consistent with previous observations (Haas et al 2007). The observed stability of Foxp3 + Helios + among CD4 + cells is also consistent with previous observations (Alexander et al 2013). …”

Low-dose interleukin-2 selectively corrects regulatory T cell defects in patients with systemic lupus erythematosus

“…As previously shown by others,16 17 percentages of total Foxp3+CD127lo Treg among CD3+CD4+ T cells were higher in patients with SLE (figure 2A), and correlated with the SLEDAI and with serum levels of anti-dsDNA antibodies (see online supplementary figure S2A), while absolute Treg numbers and Foxp3 expression levels in Treg were not different between patients with SLE and healthy controls (see online supplementary figure S2B,C). By contrast, analysis of surface expression of CD25 on Treg from patients with SLE revealed significantly lower frequencies of Treg expressing CD25 at high and intermediate levels (CD25hi and CD25int) and an increase of CD25 negative (CD25neg) cells among Treg compared with healthy controls (figure 2B).…”

IL-1R1 is expressed on both Helios+ and Helios−FoxP3+CD4+ T cells in the rheumatic joint

“…Helios appears to be associated more with the path of activation rather than the origin of these cells [16,17]. However, it has been of interest to distinguish Helios 1 from Helios 2 T regs as some functional differences have been suggested, such as the ability to produce cytokines [18,19]. Recently, surface expression of IL-1R1 (IL-1 receptor type 1) in conjunction with CCR7 has been shown to be a useful combination of cell surface markers to distinguish Helios 2 T regs from Helios 1 T cells [20].…”