Fp278characterization and Quantification of Proteasuria in Nephrotic Syndrome

Artunc, Ferruh; Alenazi, Fawza; Kalbacher, Hubert; Wörn, Matthias

doi:10.1093/ndt/gfy104.fp278

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“…Proteasuria was also shown to occur in urine samples from patients with nephrotic syndrome of a different etiology. Proteasuria was quantified using a universal protease substrate library and serine proteases were identified using proteomic profiling . In urine samples from nephrotic patients, total proteolytic activity was increased compared to that of healthy control persons .…”

Section: Proteasuria In Patients With Nephrotic Syndromementioning

confidence: 99%

“…Proteasuria was quantified using a universal protease substrate library and serine proteases were identified using proteomic profiling . In urine samples from nephrotic patients, total proteolytic activity was increased compared to that of healthy control persons . With class‐specific inhibitors, serine protease activity accounted for more than 80% of the total protease activity, with the remainder being aspartate, cysteine, and metalloproteases.…”

Section: Proteasuria In Patients With Nephrotic Syndromementioning

confidence: 99%

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Proteasuria—The impact of active urinary proteases on sodium retention in nephrotic syndrome

Artunc¹,

Wörn²,

Schork³

et al. 2019

Acta Physiologica

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Sodium retention and extracellular volume expansion are typical features of patients with nephrotic syndrome. In recent years, from in vitro data, endoluminal activation of the epithelial sodium channel (ENaC) by aberrantly filtered serine proteases has been proposed as an underlying mechanism. Recently, this concept was supported in vivo in nephrotic mice that were protected from proteolytic ENaC activation and sodium retention by the use of aprotinin for the pharmacological inhibition of urinary serine protease activity. These and other findings from studies in both rodents and humans highlight the impact of active proteases in the urine, or proteasuria, on ENaC-mediated sodium retention and edema formation in nephrotic syndrome. Targeting proteasuria could become a therapeutic approach to treat patients with nephrotic syndrome. However, pathophysiologically relevant proteases remain to be identified. In this review, we introduce the concept of proteasuria to explain tubular sodium avidity and conclude that proteasuria can be considered as a key mechanism of sodium retention in patients with nephrotic syndrome. K E Y W O R D Saprotinin, ENaC, nephrotic syndrome, proteasuria, proteinuria, proteolysis, serine protease

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Section: Proteasuria In Patients With Nephrotic Syndromementioning

confidence: 99%

Section: Proteasuria In Patients With Nephrotic Syndromementioning

confidence: 99%