2005
DOI: 10.1128/jb.187.24.8511-8515.2005
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FpvA-Mediated Ferric Pyoverdine Uptake in Pseudomonas aeruginosa: Identification of Aromatic Residues in FpvA Implicated in Ferric Pyoverdine Binding and Transport

Abstract: A number of aromatic residues were seen to cluster in the upper portion of the three-dimensional structure of the FpvA ferric pyoverdine receptor of Pseudomonas aeruginosa, reminiscent of the aromatic binding pocket for ferrichrome in the FhuA receptor of Escherichia coli. Alanine substitutions in three of these, W362, W391, and F795, markedly compromised ferric pyoverdine binding and transport, consistent with a role of FpvA in ferric pyoverdine recognition.Iron acquisition by Pseudomonas aeruginosa is often … Show more

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Cited by 17 publications
(13 citation statements)
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“…The presence of pyoverdin was further confirmed using spectrofluorimetric analysis which depicted an emission at 472 nm when excited at 398 nm [42]. Pyoverdin, enhanced by succinate [43], was produced within 8 h of growth, that is, during the late-log phase of the culture [44]. On the other hand, pyocyanin was detected only at the end of 24 h when the cells were in the stationary phase [45,46].…”
Section: Effect Of Benzoate On Pigment Productionmentioning
confidence: 94%
“…The presence of pyoverdin was further confirmed using spectrofluorimetric analysis which depicted an emission at 472 nm when excited at 398 nm [42]. Pyoverdin, enhanced by succinate [43], was produced within 8 h of growth, that is, during the late-log phase of the culture [44]. On the other hand, pyocyanin was detected only at the end of 24 h when the cells were in the stationary phase [45,46].…”
Section: Effect Of Benzoate On Pigment Productionmentioning
confidence: 94%
“…The structures of FpvA in different loading status have been solved (Cobessi et al 2005;Wirth et al 2007;Brillet et al 2007), and a large number of mutants of FpvA have been characterised by different research groups (James et al 2005;Nader et al 2007;Shen et al 2005), but their phenotypes have not been discussed in the light of the FpvA structures. Here, we correlate the effects of different mutations with the biochemical and structural data available on FpvA and then discuss the mechanisms of Pvd-Fe transport and the initiation of the signalling cascade.…”
Section: Introductionmentioning
confidence: 99%
“…Reaction mixtures were formulated and processed as described above for mexE except that an annealing temperature of 65.5°C was used. The gene was then excised from pMMB206 following EcoRIHindII digestion and cloned into plasmid miniCTX1 to generate pHF004, which was mobilized into ⌬mexT P. aeruginosa strains (45). Chromosomal inserts of miniCTX::mexT at the phage D113 attB site in these strains were selected as described previously (45), with plasmid pFLP then being used to excise miniCTX sequences (45), leaving the mexT gene in the chromosome.…”
Section: Methodsmentioning
confidence: 99%