Fra1 Activity in the Frog, Rana esculenta, Testis: A New Potential Role in Sperm Transport1

Cobellis, Gilda; Lombardi, Monica; Scarpa, Donatella; Izzo, Gaia; Fienga, Giulia; Meccariello, Rosaria; Pierantoni, Riccardo; Fasano, Silvia

doi:10.1095/biolreprod.104.036541

Cited by 14 publications

(6 citation statements)

References 31 publications

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“…Moreover, estradiol regulates the expression of Fra-1, which is located in peritubular myoid cells, efferent ducts and blood vessels and may be involved in sperm transport. Estradiol is also involved in sperm release in this species, since impairment of estrogen activity affects the detachment of spermatozoa from the Sertoli cells (Cobellis et al, 2005(Cobellis et al, , 2008. Taken together, these results suggest that anuran spermatogenesis is a complex process in which each stage can be independently regulated by different steroids.…”

Section: Introductionmentioning

confidence: 77%

Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)

Scaia

Volonteri

Czuchlej

et al. 2015

General and Comparative Endocrinology

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Section: Introductionmentioning

confidence: 77%

Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)

Scaia

Volonteri

Czuchlej

et al. 2015

General and Comparative Endocrinology

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“…Male frogs (n ¼ 6) were stimulated with homologous hypophysis homogenate to induce spermiation as previously described [42,43]. Spermatozoa were collected in their fluid from the cloacae, filtered, and analyzed under a light microscope to exclude contamination by other cell types.…”

Section: Frog Spz Collection and Treatmentsmentioning

confidence: 99%

Endocannabinoid System in Frog and Rodent Testis: Type-1 Cannabinoid Receptor and Fatty Acid Amide Hydrolase Activity in Male Germ Cells1

Cobellis¹,

Cacciola²,

Scarpa³

et al. 2006

Biology of Reproduction

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N-arachidonoylethanolamide (anandamide [AEA]) is the main endocannabinoid described to date in the testis. It exerts its effects through the activation of G-protein coupled cannabinoid receptors (CNR). However, the activity of AEA in controlling male reproduction is still poorly known. Here we provide direct evidence on the presence of the "endocannabinoid system," constituted by type-1 cannabinoid receptor (CNR1) and fatty acid amide hydrolase (FAAH), in the frog Rana esculenta testis demonstrating its expression in tubular compartment. In fact, during the annual reproductive cycle, both proteins increase in September, when the appearance of spermatids (SPT) occurs. Immunocytochemistry confirms their localization in germ cells and, in particular, in elongated SPT. Signals are still present in spermatozoa (SPZ), as demonstrated by Western blot analysis. Furthermore, the activation of CNR1 reduces sperm motility. Comparative research, carried out using mouse and rat SPZ, definitely indicates that the endocannabinoid system operates in SPZ of phylogenetically distant species. A conserved physiological role of endocannabinoid system in controlling the inhibition of sperm motility is suggested.

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“…Gonadotropins (LH and FSH) and sex steroids, including androgens and estrogens, were both considered because of their role in male reproduction of vertebrates [34][35][36][37][38][39][40][41][42][43]. LH-stimulated androgen production and action are both necessary for germ cell progression in males.…”

Section: Introductionmentioning

confidence: 99%

Low 17beta-Estradiol Levels in Cnr1 Knock-Out Mice Affect Spermatid Chromatin Remodeling by Interfering with Chromatin Reorganization

Cacciola¹,

Chioccarelli²,

Altucci³

et al. 2013

Biology of Reproduction

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The type 1-cannabinoid receptor, CNR1, regulates differentiation of spermatids. Indeed, we have recently reported that the genetic inactivation of Cnr1 in mice influenced chromatin remodeling of spermatids, by reducing histone displacement and then sperm chromatin quality indices (chromatin condensation and DNA integrity). Herein, we have studied, at both central and testicular levels, the molecular signals potentially involved in histone displacement. In particular, investigation of the neuroendocrine axis involved in estrogen production demonstrated down-regulation of the axis supporting FSH/estrogen secretion in Cnr1-knockout male mice. Conversely, Cnr1-knockout male mice treated with 17beta-estradiol showed a weak increase of pituitary Fsh-beta subunit mRNA levels and a rescue of sperm chromatin quality indices demonstrating that estrogens, possibly in combination with FSH secretion, play an important role in regulating chromatin remodeling of spermatids.

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Fra1 Activity in the Frog, Rana esculenta, Testis: A New Potential Role in Sperm Transport1

Cited by 14 publications

References 31 publications

Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)

Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)

Endocannabinoid System in Frog and Rodent Testis: Type-1 Cannabinoid Receptor and Fatty Acid Amide Hydrolase Activity in Male Germ Cells1

Low 17beta-Estradiol Levels in Cnr1 Knock-Out Mice Affect Spermatid Chromatin Remodeling by Interfering with Chromatin Reorganization

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