Proton Exchange Membrane Fuel Cells (PEMFCs) provide a reliable, pollution-free, sustainable, and stable power generating alternative to non-renewable resources, and they do not self-discharge. Proton exchange membrane fuel cells (PEMFCs) necessitate correct parameter estimates for effective investigation, modelling, and development of efficient fuel cells, highlighting the importance of exact modelling for successful use in many industries. The objective of this research is to estimate the parameters of PEMFC using a modified algorithm derived from the Ant Colony Optimization (ACO) meta-heuristic algorithm. In order to provide justification for the algorithm, it is initially benchmarked against 10 functions. The study compares the outcomes of PEMFC parameter estimation through the Dynamic Ant Colony Optimisation (DACO) algorithm with several other meta-heuristic algorithms such as Ant Colony Optimisation (ACO), Particle Swarm Optimisation (PSO), Differential Evolution (DE) algorithm, Artificial Bee Colony (ABC), and a hybrid algorithm known as Grey Wolf Optimisation - Cuckoo Search (GWOCS). The suggested algorithm's performance evaluation is based on minimising the Square Error (SSE). The proposed optimization algorithm exhibits better performance compared to alternative meta-heuristic algorithms due to its minimal SSE value. Statistical error analysis and non-parametric tests were used to evaluate the performance and efficacy of the modified algorithm based on the Ballard Mark V datasheet. The convergence curves of DACO demonstrate a faster pace of convergence compared to the other algorithms being compared.