Fractional rate of protein synthesis in liver and in individual muscles of lambs: effect of time of sampling following the use of the continuous infusion technique

Hunter, R. A.; Davey, Jane B.; Buttery, P. J.

doi:10.1017/s0021859600079582

Cited by 6 publications

(1 citation statement)

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“…Some workers have reported variations in the protein synthetic capacity of different muscles in sheep (Bryant & Smith, 1982;Bohorov et al 1987;Hunter et al 1987), but in the present experiment no differences in the FSR of the three muscles were observed for the control diet. Variations in the response of different muscles to applied treatments have also been reported (Bohorov et al 1987;Pel1 & Bates, 1987) and consequently, in the present study, three different muscles were examined in order to establish if a differential response between muscles to the three treatments imposed had occurred.…”

Section: Protein Turnover Measurementscontrasting

confidence: 80%

Nutritional and endocrinological manipulation of lean deposition in forage-fed steers

et al. 1991

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The effect of supplementing grass silage with fishmeal on growth, muscle composition and the rate of muscle protein synthesis was investigated in young Friesian steers with and without oestradiol implants. The effect of the P-adrenergic agonist cimaterol was simultaneously investigated in animals fed on silage alone. Treatments lasted for 9 or 10 weeks. Fishmeal supplementation significantly increased animal growth rates (P < 0.001) and the weights of three dissected muscles (P < 0.001) compared with the silage-fed controls. These effects were further enhanced in animals also implanted with oestradiol. Muscle weights expressed as a proportion of body-weight were increased by fishmeal, suggesting that protein deposition had been enhanced. No further increase in the proportional muscle weights was obtained with oestradiol. Muscle dry matter content tended to be increased in both implanted and nonimplanted animals receiving fishmeal compared with controls, but the proportions of protein, fat and ash were relatively constant. The intramuscular lipid composition was slightly altered by fishmeal. Muscle protein fractional synthetic rates (FSR), measured by continuous infusion of [3H]tyrosine, were increased by fishmeal in all three muscles of both implanted and non-implanted animals. There were no differences, however, due to oestradiol, over non-implanted fishmeal animals. This suggests that oestradiol may increase muscle accretion by reducing protein degradation rate. Cimaterol significantly increased longissimus dorsi (P < 0.05) and vastus lateralis (P < 0.01) muscle weights but had no effect on semitendinosus muscle weight or live-weight gain. The proportion of protein was increased (P < 0.001) and the fat content reduced (P < 0.05) in all three muscles but intramuscular lipid composition was not markedly affected. Whilst methylhistidine : creatinine excretion was reduced by cimaterol, FSR were increased in the 1. dorsi and v. lateralis muscles suggesting P-agonists have effects on both protein synthesis and protein degradation.

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Section: Protein Turnover Measurementscontrasting

confidence: 80%

Nutritional and endocrinological manipulation of lean deposition in forage-fed steers

et al. 1991

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Whole-body and tissue protein synthesis in steers losing weight on a low-protein roughage diet: the effect of trenbolone acetate

Hunter

Magner

1990

J. Agric. Sci.

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S U M M A R YSix Brahman (Bos indicus) steers implanted with 300 mg trenbolone acetate and six similar nonimplanted steers were fed a low-quality, low-protein roughage diet at 10 g dry matter/kg live weight. They were housed in individual pens for 27 days in 1987 before being placed in metabolism crates for measurement of digestibility, nitrogen retention, and the rate of protein synthesis in the whole body and fractional rate in three muscles and hide.The rate of weight loss of steers treated with trenbolone acetate was significantly (P < 005) less than that of controls (-0-34 v. -0-56 kg/day). Treated steers excreted significantly (P < 005) less 3-methylhistidine (128 v. 202 umol) and urea N (0-5 v. 1 -6 g/day) in urine. There was a more favourable nitrogen retention in animals implanted with trenbolone acetate (-10 v. -12g/day) but this difference was not significant.Whole-body protein synthesis, calculated from plasma leucine flux, was 511 and 508 g/day in treated and control steers, respectively. Treatment also had no significant effect on the fractional rate of protein synthesis, in skeletal muscle or in hide. The fractional rate of synthesis in muscle was < 1 %/day and in hide < 2%/day. These results are discussed in relation to the protein and energy metabolism of undernourished cattle. I N T R O D U C T I O Na su b m ai n tenance diet and related changes in protein metabolism to known changes in energy metabolism Beef production in seasonally dry areas follows a (Hunter & Vercoe 1987) associated with trenbolone pattern of weight gain during the wet season and zero acetate treatment. gain or weight loss during the dry season, resulting in A possible complication with trenbolone acetate is small annual gains. Improvements in production the differential effect on protein transactions in could result from an increase in gain during the wet or different muscles. It can be calculated from the a decrease in loss during the dry season. There is comparative slaughter experiment of Wood et al. evidence that the anabolic agent trenbolone acetate (1986) that a combined implant of trenbolone acetate reduces energy expenditure in cattle (Hunter & and oestradiol increased protein deposition in the Vercoe 1987), and thus has the potential to reduce neck and shoulder by an average of c. 20% and in the weight loss in the dry season. It has been suggested vertebral column and hindquarter muscles by only that part of the mode of action of trenbolone acetate c. 10%. In this paper, the effect of trenbolone acetate in the weight gain of female rats (Vemon & Buttery on protein synthesis in the whole body, in the hide 1976, \91Sa,b)and female lambs (Sinnett-Smith et al. and in three muscles was measured, and the calculated 1983) is its effect on reducing muscle protein synthesis energy costs of protein synthesis were related to and breakdown. As the reduction in breakdown is energy expenditure in the whole animal. The muscles greater than the reduction in synthesis, the net result were the trapezius in the shoulder, the longiss...

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Effect of gender, gonadectomy and oestradiol-17β on growth in lambs under grazing conditions

2001

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SUMMARYTo identify separate effects of gender, castration and exogenous oestrogen on growth, castrated lambs of both sexes and entire male lambs (n l 8) were implanted subcutaneously with three sizes of oestradiol-17β implants, or not implanted, and grazed on ryegrass and white clover pasture for 180 days. A group of non-implanted entire female lambs (n l 8) was run together with the others. Nonimplanted entire male lambs grew faster, had heavier heads, less internal, non-carcass fat and more protein and less fat and water in the carcass than non-implanted entire females. In addition, they had higher 12th vertebral spine, thicker tibia, and heavier and larger humerus than entire female lambs. Castration of male lambs reduced live-weight gain, weight of head and content of protein in the carcass whereas it increased carcass fat content. In addition, it caused lengthening of cannon bones and reduced height of 12th vertebral spine and length of tibia. In females, gonadectomy increased height of 12th vertebral spine and diameter to length ratio of the radius. Oestradiol treatment increased live-weight gain, reduced total internal and carcass fat, and increased water and protein content of the carcass in gonadectomized animals of either sex, and increased weight of carcass and head in spayed ewe lambs. Oestradiol treatment inhibited longitudinal growth of cannon bones and stimulated that of vertebral column and ribs, but had little effect on the dimensions of limb bones apart from increasing their diameter. Oestradiol treatment had no effect on muscle length but increased muscle girth and weight, except for m. splenius in ram lambs where muscle weight was reduced. Effects of oestradiol on skeletal measurements in most cases were linearly related to dose of oestradiol. It was concluded that the variable effects of sex steroids on the skeleton were related to the differential pattern of skeletal maturation. In early maturing bones acceleration of the growth process by an exogenous sex steroid caused elongation to cease prematurely, whereas in late-maturing bones the acceleration effect on elongation did not result in premature cessation. This observation may explain the often contradictory reports in the literature on the effects of sex steroids on linear growth of bone.

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Fractional rate of protein synthesis in liver and in individual muscles of lambs: effect of time of sampling following the use of the continuous infusion technique

Cited by 6 publications

References 6 publications

Nutritional and endocrinological manipulation of lean deposition in forage-fed steers

Nutritional and endocrinological manipulation of lean deposition in forage-fed steers

Whole-body and tissue protein synthesis in steers losing weight on a low-protein roughage diet: the effect of trenbolone acetate

Effect of gender, gonadectomy and oestradiol-17β on growth in lambs under grazing conditions

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