The main objective of this study was to evaluate the fruit quality characteristics such as fruit weight, dimensional fruit properties, firmness, color, and biochemical properties such as SSC, TA, vitamin C, total phenolics, total flavonoids, and antioxidant capacity of Mondial Gala, Jeromine, and Granny Smith cultivars grafted on M9 full-dwarf clonal rootstock and Granny Smith, Fuji, Galaxy Gala, Red Chief, and Scarlett Spur cultivars grafted on MM106 semi-dwarf clonal rootstock grown in Ordu (Türkiye) ecological conditions. According to the research findings, fruit weight 185.40 g (Mondial Gala/M9)-284.03 g (Scarlet Spur/MM106), firmness 69.73 N (Galaxy Gala/MM106)-84.47 N (Granny Smith/M9), SSC 8.29% (Scarlett Spur) /MM106)-14.27% (Fuji/MM106), and Vitamin C 58.00 mg (Scarlet Spur/MM106)-112.33 mg (Granny Smith/M9). In addition, it was determined that Granny Smith, Red Chief, Mondial Gala, and Jeromine cultivars stand out compared to other cultivars in terms of bioactive compounds that positively affect human health. As a result, it has been revealed that apple varieties grafted on M9 and MM106 clone rootstocks examined in the study can be grown in high quality in Ordu ecology.