“…Cholesterol, short-chain fatty acids and saturated fatty acids were enriched in the first fractions obtained at lower pressures, while long-chain fatty acids and unsaturated fatty acids were enriched in the later fractions. The melting of the last fractions occurred at higher temperatures than the melting of the first ones, as could be expected (Fatouh et al, 2007). The influence of temperature and pressure on the supercritical carbon dioxide fractionation of sheep milk fat was studied by Spano, Salis, Mele, Madau, and Mosduzzi (2004); the most promising extraction conditions for single-step fractionation comprised a temperature of 40 C and pressure of 250 bar, at which two fractions, extract and residue, with distinctively different properties were obtained with almost equal yields (Spano et al, 2004).…”