Fractionation of carboxylated carbon nanotubes and the corresponding variation in their colloidal behavior

Wu, Zheqiong; Mitra, Somenath

doi:10.1039/c4em00224e

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…2 and Table 2 ). The shorter MWCNTs showed a larger Zeta potential compared with the longer ones, which could be due to different dispersibility of MWCNTs contributed by the lengths as suggested before [ 26 , 27 ]. The results indicated that the toxicity of MWCNTs was length dependent that exposure to longer MWCNTs was associated with more morphological changes of HUVECs (Fig.…”

Section: Discussionmentioning

confidence: 65%

The adverse vascular effects of multi-walled carbon nanotubes (MWCNTs) to human vein endothelial cells (HUVECs) in vitro: role of length of MWCNTs

et al. 2017

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BackgroundIncreasing evidences indicate that exposure to multi-walled carbon nanotubes (MWCNTs) could induce adverse vascular effects, but the role of length of MWCNTs in determining the toxic effects is less studied. This study investigated the adverse effects of two well-characterized MWCNTs to human umbilical vein endothelial cells (HUVECs).MethodsThe internalization and localization of MWCNTs in HUVECs were examined by using transmission electron microscopy (TEM). The cytotoxicity of MWCNTs to HUVECs was assessed by water soluble tetrazolium-8 (WST-8), lactate dehydrogenase (LDH) and neutral red uptake assays. Oxidative stress was indicated by the measurement of intracellular glutathione (GSH) and reactive oxygen species (ROS). ELISA was used to determine the release of inflammatory cytokines. THP-1 monocyte adhesion to HUVECs was also measured. To indicate the activation of endoplasmic reticulum (ER) stress, the expression of ddit3 and xbp-1s was measured by RT-PCR, and BiP protein level was measured by Western blot.ResultsTransmission electron microscopy observation indicates the internalization of MWCNTs into HUVECs, with a localization in nuclei and mitochondria. The longer MWCNTs induced a higher level of cytotoxicity to HUVECs compared with the shorter ones. Neither of MWCNTs significantly promoted intracellular ROS, but the longer MWCNTs caused a higher depletion of GSH. Exposure to both types of MWCNTs significantly promoted THP-1 adhesion to HUVECs, accompanying with a significant increase of release of interleukin-6 (IL-6) but not tumor necrosis factor α (TNFα), soluble ICAM-1 (sICAM-1) or soluble VCAM-1 (sVCAM-1). Moreover, THP-1 adhesion and release of IL-6 and sVCAM-1 induced by the longer MWCNTs were significantly higher compared with the responses induced by the shorter ones. The biomarker of ER stress, ddit3 expression, but not xbp-1s expression or BiP protein level, was significantly induced by the exposure of longer MWCNTs.ConclusionsCombined, these results indicated length dependent toxic effects of MWCNTs to HUVECs in vitro, which might be associated with oxidative stress and activation of ER stress.Electronic supplementary materialThe online version of this article (10.1186/s12951-017-0318-x) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 65%

The adverse vascular effects of multi-walled carbon nanotubes (MWCNTs) to human vein endothelial cells (HUVECs) in vitro: role of length of MWCNTs

et al. 2017

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“…Bu nedenle ilgili çalışma kapsamında, artan nanomateryal kullanımına karşı karbon nanotüplerin (SWCNT, MWCNT ve CCNT) arpa çimlenmesi üzerinde olası etkileri araştırılmıştır. Çalışma kapsamında değerlendirilen karbon nanotüplerin fizikokimyasal özelliklerinin, bu yapıların bulundukları ortamdaki hareketlerini etkilediği, ortam koşullarına verdikleri cevabı değiştirdiği yapılan çalışmalar ile gözlenmiştir(24).…”

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The Effect of Various Carbon Nanotubes on Barley Germination

Akdemir¹,

Seven²,

Kalay³

et al. 2019

Experimed

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Objective: Carbon nanotubes (CNT), one of the most important carbon-based nanomaterials, have started to be used in the fields of biology and medicine. Drug delivery, cancer treatment, biosensors, biomedical imaging and their use as composite materials in tissue engineering are some of the biomedical applications. However, their extensive applications may increase the risk of their being released into the environment. Thus, in this study, we investigated the effects of different carbon on barley (Hordeum vulgare L. 'Zeynelağa') germination. Material and Method:In addition to usage of commercial single-walled carbon nanotubes (SWCNT) and multi-walled carbon nanotubes (MWCNT), carboxylated multi-walled carbon nanotubes (CCNT) have also synthesized carboxylated multi-walled carbon nanotubes and characterized them with FTIR spectroscopy. Later, we investigated the effect of different concentrations (50 and 100 mg/L) of carbon nanotubes on the germination of barley seeds.Results: Compared to control groups, treatment of barley seeds with different concentrations of three carbon nanotubes had no effect on barley germination. Conclusion:It was observed that tested carbon nanotubes have no toxic effect on barley germination. In addition to these data, it is also important to investigate whether there is a difference in the gene expression level in order to understand the actual effect of these materials.

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Intracellular trafficking and therapeutic outcome of multiwalled carbon nanotubes modified with cyclodextrins and polyethylenimine

Mazzaglia

Scala

Sortino

et al. 2018

Colloids and Surfaces B: Biointerfaces

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Fractionation of carboxylated carbon nanotubes and the corresponding variation in their colloidal behavior

Cited by 4 publications

References 17 publications

The adverse vascular effects of multi-walled carbon nanotubes (MWCNTs) to human vein endothelial cells (HUVECs) in vitro: role of length of MWCNTs

The adverse vascular effects of multi-walled carbon nanotubes (MWCNTs) to human vein endothelial cells (HUVECs) in vitro: role of length of MWCNTs

The Effect of Various Carbon Nanotubes on Barley Germination

Intracellular trafficking and therapeutic outcome of multiwalled carbon nanotubes modified with cyclodextrins and polyethylenimine

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