Fractionation of casein hydrolysates using polysulfone ultrafiltration hollow fiber membranes

Pouliot, Yves; Gauthier, Sylvie F.; Bard, C.

doi:10.1016/0376-7388(93)85150-u

Cited by 26 publications

(17 citation statements)

References 10 publications

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“…The molecular mass distribution profile showed higher content of larger peptides (> 2000 Da) in permeates from 10 kDa membranes. These observations are in agreement with previous findings (Pouliot et al, 1993) indicating that the MMCO of polysulfone membranes did not influence to a great extent the separation characteristics of casein hydrolysates.…”

Section: Effect Of Membrane Materials Andmmcosupporting

confidence: 83%

“…It can be suggested that by changing the pH of the media from 6.0 to 10.0, the net negative charge of the peptides the pH modifications also induced rneasurable changes in the amino acid profiles in the permeates.ln accordance with previous findings (Pouliot et al, 1993), the UF-fractionation with PS and PES membranes affected the passage of polar amino acids to a greater extent than that of non-polar amino acids. Increasing pH could have affected both basic and acidic amino acids by modifying the ionization of the side chain residues and prornoting peptide-peptide interactions.…”

Section: Effect Of Ph and Edtasupporting

confidence: 77%

“…It was observed that pH modification produced marked effect on both the flux decline upon UF and molecular mass profile of the permeates obtained. In another study (Pouliot et al, 1993), it was also shown that polysulfone exhibited specific rejective properties towards charged and hydroxylated amine acids.…”

Section: Introductionmentioning

confidence: 99%

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Separation of casein hydrolysates using polysulfone ultrafiltration membranes with pH and EDTA treatments applied

Gourley

Gauthier

Pouliot

1995

Lait

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Summary -The preparation of peptide fractions from case in hydrolysates can be achieved by the use of ultrafiltration for the removal of the enzyme from the reaction mixture and/or to obtain specifie fractions of peptides. However, the ultrafiltration of case in hydrolysates is subjected to severe fouling phenomena which affect both the flux decline and the rejection properties of the membrane. Polysulfone membrane material used in previous work showed specifie rejection properties towards charged or hydroxylated amino acids. Polysulfone and polyethersulfone fiat sheet membranes were selected for their distinctive surface properties. The ultrafiltration of a tryptic hydrolysate from sodium caseinate was studied and physico-chemical variables were introduced, namely, pH of the hydrolysate (pH 6, 8, 10) and addition of a calcium sequestrant (EDTA 20 mmol 1-1 ). Flux decline measurements, total nitrogen rejection, molecular mass distribution profile, and amino acid composition of the permeates were determined. Although the polyethersulfone membranes showed greater permeability to water (+ 15%) at 25°C th an polysulfone, no distinctive effect of membrane material was observed in the permeation flux (1 h-1 m-2 ) during ultrafiltration of the hydrolysates. The rejection coefficients were similar for both materials. It was observed that the pH and the excess of EDT A had a much stronger effect th an the mate rial on the rejection properties (nitrogen, peptides, amino acids) and the flux decline. The two materials seemed to exhibit different surface reactivity towards charged molecules.

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Section: Effect Of Membrane Materials Andmmcosupporting

confidence: 83%

Section: Effect Of Ph and Edtasupporting

confidence: 77%

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Separation of casein hydrolysates using polysulfone ultrafiltration membranes with pH and EDTA treatments applied

Gourley

Gauthier

Pouliot

1995

Lait

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“…Selective isolation of cationic amino acids and peptides by electro-membrane filtration ampholytes (amino acids, peptides and proteins) selectively from complex solutions by manipulating the charge interactions between the components and the membrane surface [4,5,7,9,[16][17][18][19]21]. This can be achieved by adjusting the pH or by adapting the salt composition of the feed.…”

Section: Original Articlementioning

confidence: 99%

Selective isolation of cationic amino acids and peptides by electro-membrane filtration

Bargeman

Dohmen-speelmans

Recio

et al. 2000

Lait

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International audienceIn the food industry there is a clear trend towards the production of speciality products with a high added value. Electro-membrane filtration (EMF) can be used to separate and concentrate these products from complex solutions. With EMF, lysine was separated from a model solution and a protein hydrolysate both containing leucine. The lysine fraction in the permeate ranged from 0.86 to 0.96. The lysine transport rate and purity were improved by increasing the potential difference from 20 V to 40 V. Reduction of the transmembrane pressure from 2 to 0.5 bar improved the purity at a practically unchanged lysine transport rate. An enriched fraction of antibacterial cationic peptides (e.g. lactoferricin-B) could be produced from a lactoferrin hydrolysate using EMF. Isolation of these bioactive peptides is normally expensive due to the complex nature of the hydrolysate feed. EMF has the potential to become an attractive (partial) isolation technology

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“…This approach can be taken further if different proteins are induced to associate with or diffuse from the micelles. For example, Pouliot et al (1993) described dissociation of β-casein from micelles of a phosphocaseinate suspension, which can be microfiltered to produce β-casein-enriched and β-casein-depleted fractions. Mehra and Kelly (2004) demonstrated fractionation of whey proteins into a retentate rich in immunoglobulins, bovine serum albumin and lactoferrin, and a permeate rich in α-lactalbumin and β-lactoglobulin.…”

Section: Fractionation Of Macromoleculesmentioning

confidence: 99%

Applications of Membrane Separation

Goulas¹,

Grandison²

2008

Advanced Dairy Science and Technology

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Fractionation of casein hydrolysates using polysulfone ultrafiltration hollow fiber membranes

Cited by 26 publications

References 10 publications

Separation of casein hydrolysates using polysulfone ultrafiltration membranes with pH and EDTA treatments applied

Separation of casein hydrolysates using polysulfone ultrafiltration membranes with pH and EDTA treatments applied

Selective isolation of cationic amino acids and peptides by electro-membrane filtration

Applications of Membrane Separation

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