Fractionation of human-serum proteins by gel filtration

Flodin, Per; Killander, Johan

doi:10.1016/0006-3002(62)90104-x

Cited by 435 publications

(129 citation statements)

References 13 publications

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“…Twelve days after infection the tumour reached the maximal volume, and after 36 days it had completely regressed. The titre of virus-neutralizing antibodies was maximal in I 36 serum, but very low in I 12 serum as previously observed (Casazza et al, 1971 Flodin and Killander (1962). Protein elution was detected by continuous monitoring of the effluent at 280 nm.…”

Section: Methodssupporting

confidence: 72%

Sensitivity to cytotoxic agents of the EMT6 tumour in vivo: tumour volume versus in vitro plating. 1. Cyclophosphamide

Twentyman¹

1977

Br J Cancer

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Summary.-Regressor serum from MSV-M-infected mice markedly reduced MSV-M oncogenesis when administered i.p. (0-1 ml/mouse) as much as 30 days before i.m. MSV-M infection in adult BALB/c mice. The regressor serum activity appeared to be directly dependent on the amount of IgG, as shown by: (1) inactivity of sera which have low virus-neutralizing antibody content; (2) high effectiveness only of the IgG serum fraction; (3) inactivity of regressor serum incubated with anti-mouse gamma-globulin serum. The regressor serum activity was specific and could not be ascribed to interferon or interferon-inducing factors, antigenantibody complexes or free antigen. The activity was not suppressed by sublethal irradiation (380 rad) of recipient mice. These results suggest that the activity of regressor serum administered before MSV-M infection is mediated through sensitization of host cells which are not radiosensitive.

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Section: Methodssupporting

confidence: 72%

Sensitivity to cytotoxic agents of the EMT6 tumour in vivo: tumour volume versus in vitro plating. 1. Cyclophosphamide

Twentyman¹

1977

Br J Cancer

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“…Thus the sera can inhibit the growth of the heterologous mutant strains in which the sugar structure 1 The reactivity of these sera against the heterologous strains might result from the fact that all the mutants used as immunogen are leaky in a strict sense and not deleted mutants (personal communication from Dr. Nikaido). …”

mentioning

confidence: 99%

“…11 serum was conducted by a modified method of Flodin and Killander [1]. Globulin fraction separated by 50% (v/v) saturation with ammonium sulfate was dialyzed against 0.01 M phosphate buffer pH 8.0, and 1.5 ml …”

mentioning

confidence: 99%

Characterization and Specificity of Anti-Bacterial Antibody toSalmonella

Nakano¹,

Kishimoto²,

Saito³

1968

Japanese Journal of Microbiology

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“…Serum proteins have been fractionated on Sephadex G-200 (Flodin and Killander, 1962). Molecules greater than about 200,000 in molecular weight do not penetrate the gel matrix and are eluted first.…”

Section: Gel Filtrationmentioning

confidence: 99%

Post‐Rigor Changes in Selected Physicochemical Properties of Myosin B Fraction of Turkey Muscle

Scharpf

Marion

Forsythe

1966

Journal of Food Science

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SUMMARY Changes in selected physicochemical properties of the myosin B fraction of turkey muscle were followed during a 44‐hr post‐rigor aging period. Sedimentation patterns of myosin B fractions prepared 48 hr after death indicated a decrease in a high‐molecular‐weight component thought to he myosin B gel. It is concluded that myosin B fractions lose gel‐forming ability with resolution of rigor. Density‐gradient centrifugal patterns showed small increases in slowly sedimenting components of myosin B fractions with aging. These components were. high in ATP‐ase activity in the presence of calcium ions and showed no reduction of viscosity with addition of ATP. Some evidence favors the concept of dissociation of myosin B into myosin A and actin with resolution of rigor. This dissociation, however, may also represent disruption of myosin R gel formed during and after extraction from muscle.

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Fractionation of human-serum proteins by gel filtration

Cited by 435 publications

References 13 publications

Sensitivity to cytotoxic agents of the EMT6 tumour in vivo: tumour volume versus in vitro plating. 1. Cyclophosphamide

Sensitivity to cytotoxic agents of the EMT6 tumour in vivo: tumour volume versus in vitro plating. 1. Cyclophosphamide

Characterization and Specificity of Anti-Bacterial Antibody toSalmonella

Post‐Rigor Changes in Selected Physicochemical Properties of Myosin B Fraction of Turkey Muscle

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