Fractionation of medium-chain-length polyhydroxyalkanoate biosynthesized by pilot-scale production for improving material properties

Panaksri, Anuchan; Tanadchangsaeng, Nuttapol

doi:10.1016/j.polymdegradstab.2023.110368

Cited by 7 publications

(2 citation statements)

References 37 publications

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“…The MCL-PHA used in the study was produced on a pilot scale based on previous studies 20 , 21 . The forming of MCL-PHA uses the chloroform solvent casting method into the mold.…”

Section: Methodsmentioning

confidence: 99%

Fabrication and biological properties of artificial tendon composite from medium chain length polyhydroxyalkanoate

Tawonsawatruk,

Panaksri,

Hemstapat

et al. 2023

Sci Rep

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Medium chain length polyhydroxyalkanoate (MCL-PHA), a biodegradable and biocompatible material, has a mechanical characteristic of hyper-elasticity, comparable to elastomeric material with similar properties to human tendon flexibility. These MCL-PHA properties gave rise to applying this material as an artificial tendon or ligament implant. In this study, the material was solution-casted in cylinder and rectangular shapes in the molds with the designated small holes. A portion of the torn human tendon was threaded into the holes as a suture to generate a composite tendon graft. The tensile testing of the three types of MCL-PHA/tendon composite shows that the cylinder material shape with the zigzag threaded three holes has the highest value of maximum tensile strength at 56 MPa, closing to the ultimate tendon tensile stress (50–100 MPa). Fibroblast cells collected from patients were employed as primary tendon cells for growing to attach to the surface of the MCL-PHA material to prove the concept of the composite tendon graft. The cells could attach and proliferate with substantial viability and generate collagen, leading to chondrogenic induction of tendon cells. An in vivo biocompatibility was also conducted in a rat subcutaneous model in comparison with medical-grade silicone. The MCL-PHA material was found to be biocompatible with the surrounding tissues. For surgical application, after the MCL-PHA material is decomposed, tendon cells should develop into an attached tendon and co-generated as a tendon graft.

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“…The MCL-PHA used in the study was produced on a pilot scale based on previous studies 20 , 21 . The forming of MCL-PHA uses the chloroform solvent casting method into the mold.…”

Section: Methodsmentioning

confidence: 99%

Fabrication and biological properties of artificial tendon composite from medium chain length polyhydroxyalkanoate

Tawonsawatruk,

Panaksri,

Hemstapat

et al. 2023

Sci Rep

Self Cite

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“…The prototypal PHA is poly(3-hydroxybutyrate) (P3HB), a homopolymer primarily obtained by using acetate as a carbon source for microorganism feeding. However, the high crystallinity, the brittleness, and a melting point close to its decomposition temperature limit its processability and use [16]. On the other hand, when microorganisms are fed with a mixture of C2-C5 volatile fatty acids, synthetic or coming from the controlled acidogenic fermentation of organic waste, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer (P3HBV) is obtained.…”

Section: Introductionmentioning

confidence: 99%

Rapid Estimation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Composition Using ATR-FTIR

Alfano,

Pagnanelli,

Martinelli

2023

Polymers

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A great research effort is involved in polyhydroxyalkanoates (PHAs) production and characterization since they are an attractive degradable polyester family that potentially could substitute oil-based polymers. This is due to two main key factors: their production is sustainable, being that they are produced by microorganisms possibly fed by organic waste-derived products, and they are degradable. Moreover, PHAs’ thermal and mechanical properties could be tuned by varying their monomeric composition through the proper selection of microorganism feedstock and bioreactor operative conditions. Hence, a rapid and facile determination of the PHA chemical structure by widely available instrumentation is useful. As an alternative to the standard gas-chromatographic method, a new procedure for the composition determination of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P3HBV), the most common PHA copolymer, by attenuated total reflection FTIR (ATR-FTIR) is presented. It is based on the linear dependence of selected and normalized absorption band intensity with the molar fraction of repeating units. To break free from the crystallinity variability, which affects the result reproducibility and data scattering, the polymer sample was rapidly quenched from the melt directly on the surface of the ATR internal reflection element and analyzed. The data obtained from 14 samples with a molar fraction of 3-hydroxybutyrate repeating units (X3HB) ranging from 0.15 to 1 were analyzed. According to preliminary analyses, the normalized intensity of two absorption bands was selected to develop a calibration method able to predict X3HB of unknown samples and to evaluate the related uncertainty through prediction intervals of inverse regression. The proposed method proves to be useful for an easy and rapid estimation of P3HBV composition.

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Recent advances on the systems metabolically engineered Pseudomonas species as versatile biosynthetic platforms for the production of polyhydroxyalkanoates

Song,

Jo,

Lee

et al. 2023

Syst Microbiol and Biomanuf

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Fractionation of medium-chain-length polyhydroxyalkanoate biosynthesized by pilot-scale production for improving material properties

Cited by 7 publications

References 37 publications

Fabrication and biological properties of artificial tendon composite from medium chain length polyhydroxyalkanoate

Fabrication and biological properties of artificial tendon composite from medium chain length polyhydroxyalkanoate

Rapid Estimation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Composition Using ATR-FTIR

Recent advances on the systems metabolically engineered Pseudomonas species as versatile biosynthetic platforms for the production of polyhydroxyalkanoates

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