Fractionation of Protein Solutions by Membrane Partition Chromatography

Blatt, William F.; Hudson, Billy G.; Robinson, Sarah; Zipilivan, E.M.

doi:10.1038/216511b0

Cited by 38 publications

(10 citation statements)

References 5 publications

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“…These membranes have been shown to be selective molecular filters. allowing passage of substances on the basis of molecular weight and configuration (15). Assuming that this property of Diaflow membranes is applicable to the conditions of this study, the results summarized in Table IV suggest that the molecular weight of the factor inhibiting SCC is probably no greater than 3000.…”

Section: Methodsmentioning

confidence: 84%

The effect of dialysates and ultrafiltrates of plasma of saline-loaded dogs on toad bladder sodium transport

Buckalew¹,

Martínez²,

Green³

1970

J. Clin. Invest.

108

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A B S T R A C T In order to obtain direct evidence for the existence of a natriuretic hormone, dialysates and ultrafiltrates of plasma of dogs expanded with saline were tested for effects on sodium transport by the toad urinary bladder. Dialysate was obtained by dialysis of blood in vivo in a clinical dialyzer and by dialysis in vitro of small volumes of blood using a miniature model of the clinical dialyzer. Ultrafiltrates were prepared using selective molecular filters which permit passage of substances on the basis of molecular weight and three dimensional configuration.Dialysates and ultrafiltrates of hydropenic dogs caused a change in toad bladder potential difference of + 1% and in short circuit current of -5%. In contrast, dialysates and ultrafiltrates from expanded dogs caused a change in potential difference of -23% and in short circuit current of -32%, a highly significant difference. Onset of reduction of short circuit current occurred within 3-5 min, reaching a maximum in 10-20 min. The effect was rapidly reversible, was specific for the serosal surface of the bladder, and could not be explained on the basis of nonspecific alterations in ionic composition or by dilutional effects. Ultrafiltrates of jugular vein plasma caused significantly more reduction of short circuit current than ultrafiltrates of femoral vein plasma. The data indicate the presence in plasma of saline-loaded dogs of a dialyzable inhibitor of toad bladder sodium transport. Ultrafiltrate studies using membranes of appropriate selectivity suggest the factor has a molecular weight of less than 3000.

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Section: Methodsmentioning

confidence: 84%

The effect of dialysates and ultrafiltrates of plasma of saline-loaded dogs on toad bladder sodium transport

Buckalew¹,

Martínez²,

Green³

1970

J. Clin. Invest.

108

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“…To characterize further the active component in the supernatant, serial fractionation by membrane partition chromatography was performed using Amicon Ultra‐15 centrifugal filter units (Millipore, Carrigtwohill, Ireland) according to the manufacturer's guidelines based on a protocol published previously [24]. In brief, dissolved yeast supernatant was spun in an Amicon Ultra‐15 centrifugal filter device with a 100 kDa nominal molecular weight limit at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Saccharomyces boulardii inhibits lipopolysaccharide-induced activation of human dendritic cells and T cell proliferation

Schlüter

Przesdzing

Metzke

et al. 2009

Clinical and Experimental Immunology

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SummarySaccharomyces boulardii (Sb) is a probiotic yeast preparation that has demonstrated efficacy in inflammatory and infectious disorders of the gastrointestinal tract in controlled clinical trials. Although patients clearly benefit from treatment with Sb, little is known on how Sb unfolds its anti-inflammatory properties in humans. Dendritic cells (DC) balance tolerance and immunity and are involved critically in the control of T cell activation. Thus, they are believed to have a pivotal role in the initiation and perpetuation of chronic inflammatory disorders, not only in the gut. We therefore decided to investigate if Sb modulates DC function. Culture of primary (native, non-monocytederived) human myeloid CD1c + CD11c + CD123 -DC (mDC) in the presence of Sb culture supernatant (active component molecular weight < 3 kDa, as evaluated by membrane partition chromatography) reduced significantly expression of the co-stimulatory molecules CD40 and CD80 (P < 0·01) and the DC mobilization marker CC-chemokine receptor CCR7 (CD197) (P < 0·001) induced by the prototypical microbial antigen lipopolysaccharide (LPS). Moreover, secretion of key proinflammatory cytokines such as tumour necrosis factor-a and interleukin (IL)-6 were notably reduced, while the secretion of anti-inflammatory IL-10 increased. Finally, Sb supernatant inhibited the proliferation of naive T cells in a mixed lymphocyte reaction with mDC. In summary, our data suggest that Sb may exhibit part of its antiinflammatory potential through modulation of DC phenotype, function and migration by inhibition of their immune response to bacterial microbial surrogate antigens such as LPS.

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“…The fraction retained by the membrane was washed with portions of distilled water until the filtrate contained 5 mg. carbon per liter (or less) and then passed through a UM-10 membrane in the same manner. Finally, after washing, the (Blatt, Feinberg, et al, 1965) and since (Michaels, 1965;Wake and Posner, 1967; Blatt, Hudson, et al, 1967). mg C =12 7.…”

Section: Um-2 Um-20 Um-20ementioning

confidence: 99%

Communication. Ultrafiltration of aquatic humus

Gjessing¹

1970

Environ. Sci. Technol.

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fluidized-bed system. These results indicate that further development of the process is warranted.Regarding future development activity, emphasis should be placed on evaluating factors such as unit capacity, heat requirements, and material losses which would provide data for economic evaluations. Because of its greater flexibility and higher unit capacity, it was concluded that further research be made with fluidized inert bed system. Future WorkA pilot-scale study of the fluidized inert bed technique is currently being initiated under contract with the FWPCA. This study will involve operation of a 10-inch-diameter fluidizedbed unit for production of 30 pounds of regenerated carbon in an 8-hour period. The unit has been designed with an integral combustion chamber in which propane will be burned with air to provide the necessary heat input and regeneration atmosphere. Feed to this unit will be partially dried spent carbon containing up to 50% moisture. The regenerated carbon produced will be recycled to FWPCA's pilot adsorption system for evaluation of the combined system performance.Results from the pilot-scale study are expected to provide the necessary data for economic evaluation and scale-up of the fluidized-bed process. AcknowledgmentThe advice and assistance of the many individuals at FWPCA who participated directly in the program are greatly appreciated.

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Fractionation of Protein Solutions by Membrane Partition Chromatography

Cited by 38 publications

References 5 publications

The effect of dialysates and ultrafiltrates of plasma of saline-loaded dogs on toad bladder sodium transport

The effect of dialysates and ultrafiltrates of plasma of saline-loaded dogs on toad bladder sodium transport

Saccharomyces boulardii inhibits lipopolysaccharide-induced activation of human dendritic cells and T cell proliferation

Communication. Ultrafiltration of aquatic humus

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