Fractionation of Snake Fruit Seeds Pondoh for Development of Anemia Treatment

Abstract

In a recent study, snake fruit seed (SFS) flour was found to enhance haemoglobin levels in a rat model of anemia. Still, it increases leucocyte number and its differential count. The purpose of this study was to fractionate SFS Pondoh and to analyze phytochemical compounds in SFS fractions. Simplicia of SFS was separated into lipid, water, and organic fractions using tetrahydrofuran, methanol and ethyl acetate reagents, and then dried using a vacuum evaporator. Chemical components in the SFS fractions were analyzed using gas chromatography-mass spectrophotometry (GC-MS). We got 77.8%, 16.5%, and 7.5% fractions of water, organic, and lipid. The water fraction contained 13 active compounds with 19 to 22 min retention time (RT). The organic fraction also had 13 active compounds with 19 to 29 min RT. The last lipid fraction contained 15 active compounds with 3 to 25 min RT. In conclusion, SFS Pondoh generates the highest water fraction, followed by organic and lipid fractions.

Nutritional Analysis of Aqueous Extract of Snake Fruit Seeds (Salacca Edulis Reinw) for Development of Anemia Treatment

“…When tannins are consumed together with iron and zinc, they will affect the bioavailability of iron and zinc. Tannins will bind non-heme iron which comes from vegetable ingredients so that iron cannot be absorbed into the body (Natasia et al 2021).…”

“…Anemia remains a global nutritional problem, which prevalence continues to increase every year (Natasia et al 2021). Asia accounts for the highest prevalence of anemia among adult women globally with a prevalence in 2018 of 46.3% and increase in 2019 to 46.6% (WHO, 2022).…”