Fractionation of tarantula venom

Atakuziev, B. U.; Barabanshchikova, N. A.; Yukel'son, L. Ya.; Tashmukhamedov, B. A.; Irgashev, R.

doi:10.1007/bf00564041

Chem Nat Compd

1974

DOI: 10.1007/bf00564041

|View full text |Cite

Fractionation of tarantula venom

B. U. Atakuziev¹,

N. A. Barabanshchikova²,

L. Ya. Yukel'son³

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2009

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

(1 citation statement)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unhindered by the somewhat primitive chromatographic techniques available, the composition of tarantula venom has been the subject of a number of early publications. Atakuziev and colleagues8 employed disk electrophoresis for the fractionation of venom from tarantulas captured in Uzbekistan. In the absence of a suitable means of detection, only basic assumptions regarding the presence of proteins, hyaluronidase and ‘toxic agents’ could be proposed for the separated components.…”

mentioning

confidence: 99%

Mass spectrometric characterisation and quantitation of selected low molecular mass compounds from the venom of Haplopelma lividum (Theraphosidae)

Moore

Smyth

Gault

et al. 2009

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

Arachnid venoms present a diverse and complex matrix for investigation, with their latent potential for innovative drug and pesticide design largely unrealised. The characterisation and quantification of selected low molecular mass compounds isolated from the crude venom of the Cobalt blue tarantula (Haplopelma lividum) were the objectives of this study. Fractionation of the crude venom was performed using reversed-phase high-performance liquid chromatography, with compound identification using both electrospray ionisation ion trap mass spectrometry and quadrupole time-of-flight mass spectrometry. Four compounds were identified, and quantification on a percentage dry weight basis was achieved by liquid chromatography/electrospray ionisation tandem mass spectrometry based on the formation of their corresponding product ions. Of these the most abundant component was glutamic acid, present at a level of 0.97%. Histamine and adenosine were detected at 0.14% and 0.10% dry weight, respectively, with the polyamine spermine noted in trace amounts at 0.002%. The limits of detection and quantification were established for each of the identified components. The fragmentation profile for histamine has also been proposed.

show abstract

mentioning

confidence: 99%

Mass spectrometric characterisation and quantitation of selected low molecular mass compounds from the venom of Haplopelma lividum (Theraphosidae)

Moore

Smyth

Gault

et al. 2009

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Fractionation of tarantula venom

Cited by 1 publication

References 3 publications

Mass spectrometric characterisation and quantitation of selected low molecular mass compounds from the venom of Haplopelma lividum (Theraphosidae)

Mass spectrometric characterisation and quantitation of selected low molecular mass compounds from the venom of Haplopelma lividum (Theraphosidae)

Contact Info

Product

Resources

About