Fractionation, partial characterization and bioactivity of water-soluble polysaccharides and polysaccharide–protein complexes from Pleurotus geesteranus

Zhang, Mei; Zhu, Lin; Cui, Steve W.; Wang, Qi; Zhou, Ting; Shen, Hengsheng

doi:10.1016/j.ijbiomac.2010.09.003

Cited by 66 publications

(34 citation statements)

References 15 publications

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“…TP for wild-growing and cultivated C. comatus were spotted at 11.84 g/100 g (Stojković et al 2013), which correlates with our results. A high correlation between TP and antioxidant activity of extracts (DPPH, FRAP tests) were recorded (Table 3), which could be possibly explained by a synergistic antioxidant activity of the protein-polysaccharide complex (Zhang et al 2011). …”

Section: Total Content Of Phenols Flavonoids and Proteinsmentioning

confidence: 89%

A comparative overview of antioxidative properties and phenolic profiles of different fungal origins: fruiting bodies and submerged cultures of Coprinus comatus and Coprinellus truncorum

et al. 2017

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Bioactive properties of fungi considerably differ between the fruiting body (FB) and the submerged culture as regards mycelia (M) and the fermentation broth (F). Antioxidant properties of hot-water extracts obtained from three different fungal origins: FB, M and F of two autochthonous fungal species (Northern Serbia), Coprinus comatus and Coprinellus truncorum were investigated. Free radical scavenging capacity (RSC) was evaluated in vitro by the DPPH assay and reducing power ability (FRAP assay). Considering possible bioactive properties of different compounds present in fungal extracts, the content of total proteins (TP), phenols (TC) and flavonoids (TF) were investigated colorimetrically. The chemical characterisation of the examined extracts was evaluated using the HPLC-MS/MS method. C. comatus showed the strongest RSC activity; more precisely, fermentation broth extract (FCc) on DPPH radicals (IC50 = 5.06 lg mL -1 ) and fruiting body extract (FBCc) for the FRAP assay (42.86 mg ascorbic acid equivalents (AAE)/g). Submerged M extract of both species showed the highest TC (MCc 81.95 mg gallic acid eq (GAE)/g d.w.; MCt 81.64 mg GAE/g d.w.), while FB extracts contained the highest content of TP. Comparing LC-MS phenolic profiles between species-interspecifically and among different fungal origins-intraspecifically (fruiting bodies and submerged cultures), high variations were noticed. In submerged M or F extracts of C. comatus, vanillic, gallic, gentisic and cinnamic acids were detected, as opposed to FB. Considering that diverse phenolic profiles of detected antioxidant compounds were obtained by submerged cultivation, this type of cultivation is promising for the production of antioxidant substances.

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Section: Total Content Of Phenols Flavonoids and Proteinsmentioning

confidence: 89%

A comparative overview of antioxidative properties and phenolic profiles of different fungal origins: fruiting bodies and submerged cultures of Coprinus comatus and Coprinellus truncorum

et al. 2017

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“…The extract of P. geesteranus has anticancer and antioxidant activities , Zhang et al 2011. However, to our knowledge, there is no report about the hypolipidemic effect of P. geesteranus polysaccharide.…”

Section: Introductionmentioning

confidence: 96%

Fermentation characteristics in stirred-tank reactor of exopolysaccharides with hypolipidemic activity produced by Pleurotus geesteranus 5#

Mao

Geng

et al. 2013

An. Acad. Bras. Ciênc.

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In this study, the hypolipidemic effect of exopolysaccharides (EPS) from Pleurotus geesteranus 5 # fermenting liquor by the optimal culture conditions in a 5-L stirred-tank reactor was investigated. The hypolipidemic effect of the polysaccharide, investigated in streptozotocin induced diabetic mice, decreased plasma glucose, total cholesterol and triacylglycerol concentrations by 17.1 %, 18.8 % and 12.0 %, respectively. The results of the present investigation strongly demonstrate the potential of this polysaccharide to prevent hyperglycemia in the experimental animals. Under optimal culture conditions, the maximum concentrations of mycelial and EPS were 22.63 g/L after 7 d cultivation and 11.09 g/L after 10 d, respectively. Furthermore, the morphological parameters (i.e. mean diameter, circularity, roughness and compactness) of the pellets and the broth viscosity were characterized. It was proved that compactness of the pellet morphology (R 2 =0.963, p<0.01) was significantly and positively determined with mycelial biomass. Moreover, mean diameter (R 2 =93.3, p<0.01) and broth viscosity (R 2 =0.950, p<0.01) were significantly and positively determined with EPS content.

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“…Although free and protein‐bound polysaccharides from mushrooms have been shown to possess important medicinal properties, there is little information in the literature about their biological and physicochemical properties 10, 11. There are some reports on the production of free and protein‐bound polysaccharides from basidiomycete strains such as C. versicolor, Pleurotus ostreatus, Lentinula edodes and Ganoderma lucidum 2, 12, 13.…”

Section: Introductionmentioning

confidence: 99%

Production, purification and characterisation of polysaccharides from Pleurotus ostreatus with antitumour activity

et al. 2012

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Fractionation, partial characterization and bioactivity of water-soluble polysaccharides and polysaccharide–protein complexes from Pleurotus geesteranus

Cited by 66 publications

References 15 publications

A comparative overview of antioxidative properties and phenolic profiles of different fungal origins: fruiting bodies and submerged cultures of Coprinus comatus and Coprinellus truncorum

A comparative overview of antioxidative properties and phenolic profiles of different fungal origins: fruiting bodies and submerged cultures of Coprinus comatus and Coprinellus truncorum

Fermentation characteristics in stirred-tank reactor of exopolysaccharides with hypolipidemic activity produced by Pleurotus geesteranus 5#

Production, purification and characterisation of polysaccharides from Pleurotus ostreatus with antitumour activity

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