Fractioning of Protein Extracts of Six Trichinella Isolates by the Methods of Isoelectrofocusing and Silver Staining

Abstract

Comparative electrophoretical studies on protein extracts from six Trichinella isolates have been carried out with the purpose of obtaining novel data about their species characterization. The methods of isofocusing and silver staining have been used. The electrophoretical analysis of the water-soluble proteins of the six isolates: ISS03, ISS13, ISS10, ISS02, ISS029, ISS035, has yielded as a result a heterogeneity both of number and electrophoretical mobility of the individual protein fractions. On the basis of the obtained results as well as on the data from studies of other authors [ 1-3 ] the six isolates under study have been established to belong to the species T. spiralis, T. pseudospiralis, T. nativa, T. britovi, T. nelsoni, T. murrelli.