The search for alternative materials that can be used for parts of aircraft hydraulic systems has led to the idea of applying S32750 duplex steel for this purpose. This steel is mainly used in the oil and gas, chemical, and food industries. The reasons for this lie in this material’s exceptional welding, mechanical, and corrosion resistance properties. In order to verify this material’s suitability for aircraft engineering applications, it is necessary to investigate its behaviour at various temperatures since aircrafts operate at a wide range of temperatures. For this reason, the effect of temperatures in the range from +20 °C to −80 °C on impact toughness was investigated in the case of S32750 duplex steel and its welded joints. Testing was performed using an instrumented pendulum to obtain force–time and energy–time diagrams, which allowed for more detailed assessment of the effect of testing temperature on total impact energy and its components of crack initiation energy and crack propagation energy. Testing was performed on standard Charpy specimens extracted from base metal (BM), welded metal (WM), and the heat-affected zone (HAZ). The results of these tests indicated high values of both crack initiation and propagation energies at room temperature for all the zones (BM, WM, and HAZ) and sufficient levels of crack propagation and total impact energies above −50 °C. In addition, fractography was conducted through optical microscopy (OM) and scanning electron microscopy (SEM), indicating ductile vs. cleavage fracture surface areas, which corresponded well with the impact toughness values. The results of this research confirm that the use of S32750 duplex steel in the manufacturing of aircraft hydraulic systems has considerable potential, and future work should confirm this.