This study (40) locally isolated the genus Streptomyces from soil samples collected from different regions of Iraq ( Nineveh , Erbil , Duhok ) and evaluated their antagonistic. The isolates were found to have bioactivity against gram-positive and negative bacteria and fungus. Streptomyces were isolated on (S.G. medium), and morphological similarities and the 16 srRNA sequencing were used to characterize them. . The results of a polymerase chain reaction (PCR) with eight strands of DNA gene picked from local bacteria isolates in a volume range of (900–1000) base pairs. The nitrogenic base sequence determined the polymerase chain reaction products of DNA samples selected from 6 local isolates. These strands preserved the employed DNA ladder volume. According to DNA Blast NCBI data, the species are Streptomyces atrovirens, Streptomyces SP.S. coeuleroubidus, and Streptomyces bellus.
Keywords. Streptomyces, Molecular, Pathogenic Microbes