Rock stability has long been a hot topic during underground energy exploitation, but the failure process of rock materials under earthquake effects is extremely complicated, and the failure mechanism still remains unclear. In order to investigate the fatigue damage and failure behavior of rocks under aftershock effects considering the post-mainshock damage states, a series of laboratory tests were conducted on marble specimens subjected to stepwise cyclic loading. Four levels of peak stress (i.e., 10, 30, 50, and 70 MPa) were applied in the first cycle, to simulate mainshock damage.The results indicate that, with the increase of initial cycle amplitude, mainshock damage has a significant effect on deformation behavior, dissipated energy, P-wave velocity, and AE characteristics of tested specimens during aftershock process. The increasing amplitude of initial cycle enhances irreversible deformation and weakens the resistance to deformation, which accelerates the expansion of specimen volume and results in the reduction of bearing capacity. Furthermore, the increasing amplitude of initial cycle obviously changes the failure morphologies and intensifies the final macro-fracture scale of tested specimens, which are verified by acoustic emission AF-RA value and b-value, respectively.