This paper describes our method, submitted to MIREX 2008 task "Multiple Fundamental Frequency Estimation & Tracking". This task restricted the problem of Multiple F0 Estimation and Tracking to three cases: i) Estimate active fundamental frequencies on a frame-by-frame basis; ii) Track note contours on a continuous time basis (as in audio-tomidi); iii) Track timbre on a continuous time basis. The presented method is the first genetic algorithm approach to multi-timbral music transcription and is based on a previously published genetic algorithm approach for automatic music transcription originally designed to transcribe piano songs. This algorithm was adopted to support different timbres.