Fragmentation of DNA in morphologically normal human spermatozoa

Avendaño, C.; Franchi, A.; Taylor, Steven L.; Morshedi, Mahmood; Bocca, Silvina; Oehninger, Sergio

doi:10.1016/j.fertnstert.2008.01.015

Cited by 130 publications

(82 citation statements)

References 40 publications

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“…To date, the published data on the relationship between sperm DNA integrity and normal morphology are conflicting. Although some studies have shown that abnormal sperm morphology and DNA damage are related [20,61] other studies have not identified a significant relationship between these parameters [34,62]. Our data are convincing in view of the fact that the study involves a large cohort of infertile couples with very well defined sperm defects.…”

Section: Discussionsupporting

confidence: 59%

Which isolated sperm abnormality is most related to sperm DNA damage in men presenting for infertility evaluation

Belloc¹,

Benkhalifa²,

Cohen-Bacrie³

et al. 2014

J Assist Reprod Genet

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Background Sperm DNA damage is common in infertile men and is associated with poor semen parameters but the impact of an isolated sperm abnormality on sperm DNA damage has not been studied. Objective To evaluate sperm DNA damage in a large cohort of infertile men with isolated sperm defects. Design, setting and participants Retrospective study of 1084 consecutive, non-azoospermic infertile men with an isolated sperm defect: isolated oligozoospermia (iOligo), isolated asthenozoospermia (iAstheno) or isolated teratozoospermia (iTerato). Outcome measurements and statistical analysis We examined and compared clinical parameters, conventional semen parameters and %sperm DNA fragmentation (%SDF, assessed by flow cytometry-based Terminal deoxynucleotidyl transferase-mediated dUTP Nick End-Labeling assay) in the three groups of men. Results and limitations The mean (±SD) %SDF was significantly higher in the iAstheno compared to the iOligo and iTerato groups (25.0±14.0 vs. 19.2±11.6 and 20.7±12.1 %, respectively, P<0.0001). Similarly, the proportion of men with high %SDF (>30 %) was significantly higher in the iAstheno compared to the iOligo and iTerato groups (31 % vs. 18 % and 19 %, respectively, P<0.0001). In the group of 713 men with iAstheno, %SDF was positively correlated with paternal age (r=0.20, P<0.0001) and inversely correlated with %progressive motility (r=−0.18, P<0.0001). In the subset of 218 men with iTerato, %SDF was also positively correlated with paternal age (r=0.15, P=0.018) and inversely correlated with %progressive motility (r=−0.26, P=0.0001). Conclusions In this large cohort of infertile men with isolated sperm abnormalities, we have found that the sperm DNA fragmentation level is highest in the men with sperm motility defects and that 31 % of these men have high levels of sperm DNA fragmentation. The data indicate that poor motility is the sperm parameter abnormality most closely related to sperm DNA damage.

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Section: Discussionsupporting

confidence: 59%

Which isolated sperm abnormality is most related to sperm DNA damage in men presenting for infertility evaluation

Belloc¹,

Benkhalifa²,

Cohen-Bacrie³

et al. 2014

J Assist Reprod Genet

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“…Moreover, objective evaluation criteria of clinical ICSI treatment are lacking due to nonuniform criteria among different technicians and different centers for the selection of sperms with normal morphology and strong mobility. During natural fertilization, only structurally sound sperms can complete the whole process [23] because sperms must have an intact acrosome to bind the ZP and trigger acrosomal exocytosis, and this process exposes specific regions at the sperm surface to interact with the oocyte [24,25]. Therefore, as suggested by Braga et al [25], sperm-ZP binding might be the best way to identify the most competent sperms for ICSI.…”

Section: Discussionmentioning

confidence: 99%

Outcomes of intracytoplasmic sperm injection using the zona pellucida-bound sperm or manually selected sperm

Jin¹,

Bao

Tang

et al. 2016

J Assist Reprod Genet

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Purpose Zona pellucida (ZP)-bound sperm used for intracytoplasmic sperm injection (ICSI) enhances embryo quality, implantation, and clinical pregnancy rates. This study aimed to assess the pregnancy outcomes and clinical significance of ICSI with ZP-bound sperm. Method A total of 84 infertile couples who underwent cycles of ICSI following failed in vitro fertilization between June 2012 and February 2014 were enrolled and randomized (1:1): in the treatment group, ICSI was performed using ZPbound sperm; in the control group, ICSI was performed in a standard manner. Rates of fertilization, cleavage, high-quality embryos, and clinical pregnancy were compared between the two groups. Results There were no significant differences in age, infertile period, gonadotrophin dose, number of metaphase II oocytes, and number of embryo transfers between the two groups (P > 0.05). The clinical pregnancy rate was higher in the treatment group than in the control group, but without statistical significance (60.5 vs. 47.6 %, P > 0.05). No significant differences in the rates of fertilization and cleavage were observed (83.0 vs. 81.6 %, and 96.3 vs. 96.5 %, both P > 0.05), but higher rates of high-quality embryos and useable embryos were observed with ZP-bound sperm compared with controls (66.1 vs. 50.8 % and 76.0 vs. 66.3 %, both P < 0.05). Conclusions ICSI using ZP-bound sperm might increase the embryo quality and number of useable embryos, possibly improving the clinical pregnancy outcome of ICSI.

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“…68 It has recently been reported in infertile men that spermatozoa with apparently normal morphology may have DNA fragmentation. 67,69 This finding raised the possibility that spermatozoa with normally shaped heads but with DNA fragmentation could be mistakenly selected for oocyte injection during ICSI. 70 This concern is clinically significant because the presence of an increased proportion of normal spermatozoa with damaged DNA is negatively associated with embryo quality and also pregnancy outcome after ICSI.…”

Section: Morphology and Dna Statusmentioning

confidence: 99%

“…71 We propose that the evaluation of DNA fragmentation in morphologically normal spermatozoa obtained from a separated motile fraction is apparently, the closest, albeit indirectly, way to reflect DNA normality. 69,70 This can be done through simultaneous assessment of morphology and DNA status with phase contrast and immunofluorescent microscopy, in a relatively fast and technically easy fashion.…”

Section: Morphology and Dna Statusmentioning

confidence: 99%

Semen analysis and sperm function testing

Franken¹,

Oehninger²

2011

Asian J Androl

Self Cite

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Despite controversy regarding the clinical value of semen analysis, male fertility investigation still relies on a standardized analysis of the semen parameters. This is especially true for infertility clinics in both developing and developed countries. Other optional tests or sophisticated technologies have not been widely applied. The current review addresses important changes in the analysis of semen as described in the new World Health Organization (WHO) manual for semen analysis. The most important change in the manual is the use of evidence-based publications as references to determine cutoff values for normality. Apart from the above mentioned changes, the initial evaluation and handling methods remain, in most instances, the same as in previous editions. Furthermore, the review evaluates the importance of quality control in andrology with emphasis on the evaluation of sperm morphology. WHO sperm morphology training programmes for Sub-Saharan countries were initiated at Tygerberg Hospital in 1995. The external quality control programme has ensured that the majority of participants have maintained their morphological reading skills acquired during initial training. This review reports on current sperm functional tests, such as the induced acrosome reaction, and sperm-zona pellucida binding assays, as well as the impact of sperm quality in terms of DNA integrity, and the relationship of sperm function tests to sperm morphology.

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Fragmentation of DNA in morphologically normal human spermatozoa

Cited by 130 publications

References 40 publications

Which isolated sperm abnormality is most related to sperm DNA damage in men presenting for infertility evaluation

Which isolated sperm abnormality is most related to sperm DNA damage in men presenting for infertility evaluation

Outcomes of intracytoplasmic sperm injection using the zona pellucida-bound sperm or manually selected sperm

Semen analysis and sperm function testing

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