Presented paper deals with optimization of technological processes, particularly it is focused at quality control. Quality measuring is fundamental for any quality or process improvement. "If is not possible to measure it, it is not possible to manage it." In general it is possible to say that quality is measured at the process input, during the process (in-process control) and at the process output. This paper is focused at the in-process control and its quality itself. Paper presents the case study of optimization of the in-process control by using Six Sigma methods and tools in company producing medical technology and medical products in Czech Republic. Firstly, the description and analysis of in-process control is carried out, while the problem-causing factors are examined. Based on these findings, the proposals for process optimization follow. According to methodology, the DMAIC cycle is completed by the evaluation of process performance, where partial process performance indicators are designed.