Frankia colletiae sp. nov., a nitrogen-fixing actinobacterium isolated from Colletia cruciata

Abstract

A nitrogen-fixing actinobacterium strain (Cc1.17T) isolated from a root nodule of Colletia cruciata was subjected to polyphasic taxonomic studies. The strain was characterized by the presence of meso-diaminopimelic acid in its peptidoglycan, galactose, glucose, mannose, rhamnose, ribose and xylose as cell-wall sugars, phosphatidylinositol, diphosphatidylglycerol, glycophospholipids, phosphatidylglycerol, glycophospholipid and uncharacterized lipids as its polar lipids, and C16 : 0, iso-C16 : 0, C17 : 1 ω9 and C18 : 1 ω9 as major fatty acids (>10 %). Strain Cc1.17T showed 16S rRNA gene sequence similarities of 97.4–99.8 % to validly named Frankia species. Phylogenetic trees based on 16S rRNA gene and genome sequences placed strain Cc1.17T in a new lineage within the genus Frankia . Digital DNA–DNA hybridization and average nucleotide identity values between strain Cc1.17T and its closest phylogenomic neighbours were well below the thresholds recommended for prokaryotic species delineation. Therefore, strain Cc1.17T (=DSM 43829T=CECT 9313T) merits recognition as the type strain of a new species for which the name Frankia colletiae sp. nov. is proposed.

Genome analysis and biogeographic distribution of the earliest divergent Frankia clade in the southern hemisphere

“… 2018b ), and Frankia colletiae Cc1.17 T (Nouioui et al. 2023a ). Cluster-1 is represented by Frankia casuarinae CcI3 T (Nouioui et al.…”

Frankia nepalensis sp. nov., a non-infective non-nitrogen-fixing isolate from root nodules of Coriaria nepalensis Wall

“…Frankia casuarinae CcI3 T [7], Frankia canadensis ARgP5 T [9], Frankia gtarii Agncl-4 T [10], Frankia tisai Agncl-8 T [10], Frankia umida Ag45/Mut15 T [11], Frankia coriariae BMG5.1 T [12], Frankia elaeagni BMG5.12 T [7], Frankia discariae BCU110501 T [13], Frankia soli NRRL B-16219 T [14], Frankia irregularis G2 T [15], Frankia asymbiotica M16386 T [16], Frankia saprophytica CN 3 T [17] and Frankia colletiae Cc1.17 T [18]. Additional species to describe uncultured Frankia populations in root nodules have been proposed as 'Candidatus Frankia alpina' [19], 'Candidatus Frankia nodulisporulans' [20], 'Candidatus Frankia californiensis' Dg2 [21], 'Candidatus Frankia datiscae' Dg1 [22] and 'Candidatus Frankia meridionalis' Cppng1 [23].…”

“…Since the first report on the isolation of members of the genus Frankia from root nodules in 1978 [4], many isolates representing nitrogen-fixing members of the genus have been obtained from several host plants [5, 6]. Characterization of these infective isolates resulted in the description of 15 species, including Frankia alni ACN14a T [7], Frankia torreyi CpI 1 T [8], Frankia casuarinae CcI3 T [7], Frankia canadensis ARgP5 T [9], Frankia gtarii Agncl-4 T [10], Frankia tisai Agncl-8 T [10], Frankia umida Ag45/Mut15 T [11], Frankia coriariae BMG5.1 T [12], Frankia elaeagni BMG5.12 T [7], Frankia discariae BCU110501 T [13], Frankia soli NRRL B-16219 T [14], Frankia irregularis G2 T [15], Frankia asymbiotica M16386 T [16], Frankia saprophytica CN 3 T [17] and Frankia colletiae Cc1.17 T [18]. Additional species to describe uncultured Frankia populations in root nodules have been proposed as ' Candidatus Frankia alpina' [19], ' Candidatus Frankia nodulisporulans' [20], ' Candidatus Frankia californiensis' Dg2 [21], ' Candidatus Frankia datiscae' Dg1 [22] and ' Candidatus Frankia meridionalis' Cppng1 [23].…”