Frankia KB5 Possesses a Hydrogenase Immunologically Related to Membrane-Bound [NiFe]-Hydrogenases

Mattsson, Ulrika; Johansson, Lenore; Sandström, Gunnar; Sellstedt, Anita

doi:10.1007/s002840010244

“…60 kDa. This polypeptide corresponded to the large hydrogenase subunit of Frankia strains UGL011102, AVCI1 and KB5 (results not shown), a finding that is supported by earlier studies [7].…”

Section: Resultssupporting

confidence: 85%

“…The presence of hydrogenase in some Frankia strains has also been indicated by immunological studies [7]. Previous immunogold localization studies have shown that hydrogenase is localized in both hyphae and vesicles in Frankia [8].…”

Section: Introductionmentioning

confidence: 85%

Molecular characterization of uptake hydrogenase in Frankia

Leul

¹

,

Mattsson

²

,

Sellstedt

³

2005

Biochemical Society Transactions

1

0

View full text Add to dashboard Cite

A molecular characterization of uptake hydrogenase in Frankia was performed by using two-dimensional gel electrophoresis, matrix-assisted laser-desorption ionization-time-of-flight mass spectrometry, PCR amplification and Southern blotting. A polypeptide of approx. 60 kDa was recognized in Frankia UGL011102, AVCI1 and KB5 on the two-dimensional gel by blotting with Ralstonia eutropha (Hox G) antibody. Further analysis by MS resulted in a peptide 'fingerprint', which was similar to the membrane-bound hydrogenase 2 large subunit (HYD2) in Escherichia coli. In addition, a 127 bp PCR fragment could also be amplified from Frankia AVCI1, which gave a 76% similarity with the large subunit of hydrogenase in, e.g., Azotobacter chrococcum, Bradyrhizobium japonicum and Rhizobium leguminosarum. Although immunological similarity between the small subunit of Frankia hydrogenase and that of other organisms has not yet been found, a PCR product of 500 bp could be amplified from the local source of Frankia, the analysis of which gave 69 and 67% identity with the small subunit of hydrogenases in B. japonicum and R. leguminosarum respectively. A Southern-blot analysis further indicated evidence for the presence of the small hydrogenase subunit in other Frankia strains, i.e. KB5, AvcI1 and CcI3.

show abstract

“…Likewise, uptake hydrogenase activity is widespread in Frankia (Benson et al, 1980;Mattsson and Sellstedt, 2002;Roelofsen and Akkermann, 1979). Antisera raised against the large subunit of Bradyrhizobium japonicum hydrogenase recognized this protein in Frankia KB5 (Mattsson et al, 2001). Recently the two filamentous heterocystous cyanobacteria Anabaena strain PCC 7120 and the symbiotically competent Nostoc punctiforme (Nostoc PCC 73102), have been shown to contain uptake hydrogenases coded for by the hupSL genes (Tamagnini et al, 2002).…”

Section: Evolutionary and Ecological Considerationsmentioning

confidence: 99%

Root-based N2-fixing Symbioses: Legumes, Actinorhizal Plants, Parasponia sp. and Cycads

Vessey

¹

,

Pawlowski

²

,

Bergman

³

2005

View full text Add to dashboard Cite

In the mutualistic symbioses between legumes and rhizobia, actinorhizal plants and Frankia, Parasponia sp. and rhizobia, and cycads and cyanobacteria, the N 2 -fixing microsymbionts exist in specialized structures (nodules or cyanobacterial zones) within the roots of their host plants. Despite the phylogenetic diversity among both the hosts and the microsymbionts of these symbioses, certain developmental and physiological imperatives must be met for successful mutualisms. In this review, phylogenetic and ecological aspects of the four symbioses are first addressed, and then the symbioses are contrasted and compared in regard to infection and symbio-organ development, supply of carbon to the microsymbionts, regulation of O 2 flux to the microsymbionts, and transfer of fixed-N to the hosts. Although similarities exist in the genetics, development, and functioning of the symbioses, it is evident that there is great diversity in many aspects of these root-based N 2 -fixing symbioses. Each symbiosis can be admired for the elegant means by which the host plant and microsymbiont integrate to form the mutualistic relationships so important to the functioning of the biosphere.

show abstract

“…The ATP and reductant utilized to form this H 2 by-product can be seen as lost energy, as there is no apparent useful function for H 2 in the microsymbiont or the host. Antisera raised against the large subunit of Bradyrhizobium japonicum hydrogenase recognized this protein in Frankia KB5 (Mattsson et al, 2001). Uptake hydrogenase is widespread in rhizobia (Baginsky et al, 2002), and the small and large subunits of this heterodimeric enzyme are coded for by the hupS and hupL genes, respectively.…”

Section: Evolutionary and Ecological Considerationsmentioning

confidence: 99%

Root-based N2-fixing symbioses: Legumes, actinorhizal plants, Parasponia sp. and cycads

Vessey

¹

,

Pawlowski

²

,

Bergman

³

2005

View full text Add to dashboard Cite

In the mutualistic symbioses between legumes and rhizobia, actinorhizal plants and Frankia, Parasponia sp. and rhizobia, and cycads and cyanobacteria, the N 2 -fixing microsymbionts exist in specialized structures (nodules or cyanobacterial zones) within the roots of their host plants. Despite the phylogenetic diversity among both the hosts and the microsymbionts of these symbioses, certain developmental and physiological imperatives must be met for successful mutualisms. In this review, phylogenetic and ecological aspects of the four symbioses are first addressed, and then the symbioses are contrasted and compared in regard to infection and symbio-organ development, supply of carbon to the microsymbionts, regulation of O 2 flux to the microsymbionts, and transfer of fixed-N to the hosts. Although similarities exist in the genetics, development, and functioning of the symbioses, it is evident that there is great diversity in many aspects of these root-based N 2 -fixing symbioses. Each symbiosis can be admired for the elegant means by which the host plant and microsymbiont integrate to form the mutualistic relationships so important to the functioning of the biosphere.

show abstract

Frankia KB5 Possesses a Hydrogenase Immunologically Related to Membrane-Bound [NiFe]-Hydrogenases

Cited by 12 publications

References 18 publications

Molecular characterization of uptake hydrogenase in Frankia

Molecular characterization of uptake hydrogenase in Frankia

Root-based N2-fixing Symbioses: Legumes, Actinorhizal Plants, Parasponia sp. and Cycads

Root-based N2-fixing symbioses: Legumes, actinorhizal plants, Parasponia sp. and cycads

Contact Info

Product

Resources

About