2019
DOI: 10.1101/664649
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Frataxin deficiency induces lipid accumulation and affects thermogenesis in brown adipose tissue

Abstract: 26Decreased expression of the mitochondrial protein frataxin (FXN) causes Friedreich's ataxia 27 (FRDA). FRDA is a neurodegenerative disease also characterized by systemic metabolic alterations 28 that increase the risk of developing type 2 diabetes thus aggravating FRDA prognosis. Brown adipose 29 tissue (BAT) is a mitochondria-enriched and anti-diabetic tissue that, in addition to its 30 thermoregulatory role, turns excess energy into heat to maintain energy balance. Here we report that 31FXN knock-in/knock-… Show more

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“…Then, 1 mg of RNA was treated with genomic DNase and retro-transcripted using PrimeScript RT Reagent Kit (Takara Bio Inc., Japan). qPCR was performed in triplicate on 50 ng of cDNA using validated qPCR primers (BLAST), Applied BiosystemsÔ PowerÔ SYBRÔ Green Master Mix, and the QuantStudio3 Real-Time PCR System (Thermo Fisher, Waltam, MA, USA), as described by Turchi et al (2020). The primers used for qPCR are listed below:…”
Section: Qpcr Gene Expression Analysismentioning
confidence: 99%
“…Then, 1 mg of RNA was treated with genomic DNase and retro-transcripted using PrimeScript RT Reagent Kit (Takara Bio Inc., Japan). qPCR was performed in triplicate on 50 ng of cDNA using validated qPCR primers (BLAST), Applied BiosystemsÔ PowerÔ SYBRÔ Green Master Mix, and the QuantStudio3 Real-Time PCR System (Thermo Fisher, Waltam, MA, USA), as described by Turchi et al (2020). The primers used for qPCR are listed below:…”
Section: Qpcr Gene Expression Analysismentioning
confidence: 99%