Fraud identification in industrial meat products by multiplex PCR assay

Ghovvati, Shahrokh; Nassiri, Mohammadreza; Mirhoseini, Seyed Ziaeddin; Moussavi, Alireza Heravi; Javadmanesh, Ali

doi:10.1016/j.foodcont.2008.09.002

Cited by 127 publications

(81 citation statements)

References 26 publications

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“…This is because the processed meat-based foods have a variety of flavors and forms of interest, relatively long shelf time, and relatively cheaper than the price of fresh meat, even some parties claimed that this processed meat-based food can also meet the nutritional needs well. [1,2] Manufacturers of food and processed meat products have been produced by many parties. From the level of large producers (factories) to home industries.…”

Section: Introductionmentioning

confidence: 99%

“…This quality assurance is indicated by the label or description on the packaging of the content or composition contained in the product. [1,3] Some studies in the world show the possibility of inconsistency between the composition on the label compared with the real composition contained in the product. Some cases that have been found include the mixing or substitution of raw materials of meat from other animals.…”

Section: Introductionmentioning

confidence: 99%

“…Losses in the health sector can also be experienced by consumers, because the mixing of substitutes by unhealthy raw materials can be a source of disease for consumers. [1,[3][4][5] The case of substitution of meat raw materials also occurs in Indonesia and is quite common. Some recent issues indicate the substitution of beef with mutton meat to make meatballs, for economic reasons.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Species in Meat Products in Bandung City using Multiplex PCR

Yelliantty

Rohima²

2018

PFTJ

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Cases concerning the substitution of meat raw material also occur in Indonesia and are quite common. Therefore, careful monitoring and control that needs to be done on the meat products. Screening or sampling products on the market should be conducted periodically to ensure the safety of consumers and society in general. Such screening should be done accurately. This study aimed to analyze the composition of meat in processed products in traditional markets in Bandung using PCR method. This study was using four specific primers to detect four different species. Screening is done on samples of meatballs from several markets. The results showed the presence of several samples that contain meat of some species. based it can be concluded that the substitution of raw materials processed meat products also occurs in the traditional market in the city of Bandung, and the PCR method referred to can be used as the basis for the development of detection methods of food security in Indonesia.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of Species in Meat Products in Bandung City using Multiplex PCR

Yelliantty

Rohima²

2018

PFTJ

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“…Phone: +36-52-508 444/88199; fax: +36-52-486 285;e-mail: czegledi@agr.unideb.hu Acta Alimentaria 45, 2016 Papers studied variable and conserved regions of mitochondrial genes, such as 12S rRNA (LÓPEZ-CALLEJA et al, 2005, 16S rRNA (GHOVVATI et al, 2009;NATONEK-WIŚNIEWSKA et al, 2013), cytochrome b (DOOSTI et al, 2014, cytochrome oxydase subunit I (HAIDER et al, 2012), D-loop (MANE et al, 2009) sequences with duplex-, multiplex-PCR, PCR-RFLP, and DNA sequencing.…”

mentioning

confidence: 99%

Applicability and sensitivity of PCR-SSCP method for milk species identification in cheese

Csikós¹,

Hodžić

Pasic-Juhas

et al. 2016

Acta Alimentaria

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Species identifi cation in food has become a prominent issue in recent years as the importance of consumer protection has increased. DNA-based species identifi cation methods were developed by researchers in the last two decades, as these are reliable, accurate, and low-cost techniques for species identifi cation in raw and processed food products as well. In our study, universal primers were designed to conserved regions of mitochondrial 12S rRNA. Amplicons were heat-denatured and a PCR single strand conformation polymorphism (SSCP) method was developed to identify cattle, buffalo, sheep, and goat DNA. Sensitivity of this technique was tested on DNA mixtures of cattle-sheep, cattle-goat, and cattle-buffalo and the threshold limit of cattle DNA was 5%, 5%, and 3%, respectively. One hundred and fi ve cheeses were purchased and collected from Bosnian and Hungarian farmers, retails, and supermarkets to reveal fraud, 32 percent of them (34 cheeses) were found to be mislabelled by species.

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“…The integration of species determination and nuclear DNA quantification into a single assay will streamline downstream genotyping analysis, and improve the quality of the DNA profiles while conserving reagents (Evans et al 2007;Lindquist et al 2011;Kanthaswamy et al 2012). Many of the DNA-based food authentication assays are multiplexed end-point PCR assays (Dalmasso et al 2004;Zha et al 2010;Ghovvati et al 2009;Rodriguez et al 2003). A majority of these protocols require subsequent steps such as restriction enzyme digestion or gel electrophoresis for species identification after PCR.…”

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confidence: 99%

A nuclear DNA-based species determination and DNA quantification assay for common poultry species

Satkoski

Premasuthan

et al. 2012

J Food Sci Technol

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DNA testing for food authentication and quality control requires sensitive species-specific quantification of nuclear DNA from complex and unknown biological sources. We have developed a multiplex assay based on TaqMan® realtime quantitative PCR (qPCR) for species-specific detection and quantification of chicken (Gallus gallus), duck (Anas platyrhynchos), and turkey (Meleagris gallopavo) nuclear DNA. The multiplex assay is able to accurately detect very low quantities of species-specific DNA from single or multispecies sample mixtures; its minimum effective quantification range is 5 to 50 pg of starting DNA material. In addition to its use in food fraudulence cases, we have validated the assay using simulated forensic sample conditions to demonstrate its utility in forensic investigations. Despite treatment with potent inhibitors such as hematin and humic acid, and degradation of template DNA by DNase, the assay was still able to robustly detect and quantify DNA from each of the three poultry species in mixed samples. The efficient species determination and accurate DNA quantification will help reduce fraudulent food labeling and facilitate downstream DNA analysis for genetic identification and traceability. ). The integration of species determination and nuclear DNA quantification into a single assay will streamline downstream genotyping analysis, and improve the quality of the DNA profiles while conserving reagents (Evans et al. 2007;Lindquist et al. 2011;Kanthaswamy et al. 2012). Many of the DNA-based food authentication assays are multiplexed end-point PCR assays (Dalmasso et al. 2004;Zha et al. 2010;Ghovvati et al. 2009;Rodriguez et al. 2003). A majority of these protocols require subsequent steps such as restriction enzyme digestion or gel electrophoresis for species identification after PCR. Endpoint PCR assays with a quantification component have been developed, but amplicon quantities are typically determined using imaging software analysis of fluorescence intensities of electrophoresis gel bands (Soares et al. 2010).Like end-point PCR, real-time quantitative PCR (qPCR) technology allows multiplexing, and is amenable to detecting multiple DNA targets that allow the simultaneous identification of DNA originating from multiple species. Moreover, the real-time DNA quantification feature of qPCR technology facilitates the efficient and accurate quantification of template DNA. While simplifying data collection and analysis, qPCR's ability to amplify, identify, and quantify in a single step effectively minimizes turnaround time and exposure to contamination and errors ).Electronic supplementary material The online version of this article

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Fraud identification in industrial meat products by multiplex PCR assay

Cited by 127 publications

References 26 publications

Analysis of Species in Meat Products in Bandung City using Multiplex PCR

Analysis of Species in Meat Products in Bandung City using Multiplex PCR

Applicability and sensitivity of PCR-SSCP method for milk species identification in cheese

A nuclear DNA-based species determination and DNA quantification assay for common poultry species

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