Fraxetin prevented sodium fluoride-induced chronic pancreatitis in rats: Role of anti-inflammatory, antioxidant, antifibrotic and anti-apoptotic activities

Balaha, Mohamed; Ahmed, Nehad J.; Geddawy, Ayman; Kandeel, Samah

doi:10.1016/j.intimp.2021.107372

Cited by 15 publications

(11 citation statements)

References 48 publications

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“…Moreover, the increase in the energy during the entire simulated timescale of about 100 ns is substantial proof that fluoride can activate the lipase enzyme via opening the lid located at the entry of the catalytic site. Our report is in alignment with recent studies by 28 that the pancreatic lipase is activated by the fluoride at high concentrations.…”

Section: Discussionsupporting

confidence: 93%

“…26 The findings from, 27 reveal necrotizing pancreatitis after formic acid and hydrofluoric acid burns. 27 Fluoride ingestion causes pancreatitis, 28 elevating the activity of pancreatic lipase. During pancreatitis, pancreas triglyceride lipase leaking into visceral adipose tissue can cause excessive visceral adipose tissue lipolysis and thereby worsen organ failure.…”

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confidence: 99%

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An in‐silico investigation of fluoride ions impact on pancreatic lipase

Yadav

Basha²,

Pindi

2022

J of Cellular Biochemistry

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Fluorine is a halogen beneficial to teeth and bones at a lower concentration. But in excess, it is a toxin and causes adverse effects. Fluoride is toxic to enzymes generally when it inhibits the enzyme activity involved in metabolic pathways. Here we study invitro and invivo findings on the interaction of fluoride on the enzymes Aconitase, Adenylyl cyclase, Arginase, Cytochrome‐c‐oxidase, Glucose‐6‐phosphatase, Protein phosphatase, Succinate dehydrogenase from liver and lipase from pancreas by using molecular docking and simulations to gain insights into the mechanism by which fluoride modifies the activity of pancreatic lipase. our molecular modeling and docking studies identified that lipase is the most strongly inhibited enzyme compared to other enzymes mentioned above with −0.42 Kcal/mol binding energy and 495.78 milli molar of predicted IC50 value with interaction with Phe227 residue. To further validate this, we have taken the lipase enzyme in presence of fluoride ions for molecular dynamic simulations of 100 ns. To analyze the impact of fluoride ions on the lipase dynamics, two different simulations of 100 ns each were performed. In one simulation, we have simulated lipase in its apo form in the aqueous environment without any fluoride ions and in another simulation lipase in its apo form was kept in the presence of randomly placed fluoride ions countered with sodium ions to maintain the pH as neutral. The simulation analysis revealed that major fluctuations in lipase was observed between 230 and 300 residues in presence of fluoride ions. Interestingly, this is the exact location of the “lid” like acting loop of residues responsible for the inward/outward movement of the substrate to lipase catalytically active site containing catalytic triad of residues Leu153, His263, and Pro177. His263 residue random flip is believed to be the critical incident that causes the substrate's inward/outward movement at the catalytically active site coordinated by “lid” opening, providing enough space for the substrate.

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Section: Discussionsupporting

confidence: 93%

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confidence: 99%

An in‐silico investigation of fluoride ions impact on pancreatic lipase

Yadav

Basha²,

Pindi

2022

J of Cellular Biochemistry

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“…By day 28, the two groups did not differ significantly, which is in line with some earlier reports (62)(63)(64). However, BWG seems to depend on the NaF dose, as some authors (65) reported higher BWG than ours at a lower NaF concentration of 50 mg/L NaF over 11 weeks, and others (66) reported lower BWG at NaF concentration of 500 mg/L over 60 days. Interestingly, BWG in the F+Se group was lower than control and/or the Se group throughout the experiment.…”

Section: Body Weight Gain and Water Consumptionsupporting

confidence: 91%

Redox and biometal status in Wistar rats after subacute exposure to fluoride and selenium counter-effects

Radovanović

Antonijević

Baralić

et al. 2022

Archives of Industrial Hygiene and Toxicology

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This study aimed to investigate the effect of 150 mg/L sodium fluoride (NaF) on redox status parameters and essential metals [copper (Cu), iron (Fe), and zinc (Zn)] in the blood, liver, kidney, brain, and spleen of Wistar rats and to determine the protective potential of selenium (Se) against fluoride (F-) toxicity. Male Wistar rats were randomly distributed in groups of five (n=5) receiving tap water (control) or water with NaF 150 mg/L, NaF 150 mg/L + Se 1.5 mg/L, and Se 1.5 mg/L solutions ad libitum for 28 days. Fluorides caused an imbalance in the redox and biometal (Cu, Fe, and Zn) status, leading to high superoxide anion (O2 .-) and malondialdehyde (MDA) levels in the blood and brain and a drop in superoxide dismutase (SOD1) activity in the liver and its increase in the brain and kidneys. Se given with NaF improved MDA, SOD1, and O2 .- in the blood, brain, and kidneys, while alone it decreased SH group levels in the liver and kidney. Biometals both reduced and increased F- toxicity. Further research is needed before Se should be considered as a promising strategy for mitigating F- toxicity.

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“…In R. okamurae ( Table 3 ), the majority of the most abundant molecular formulas were present in all extracts, except the aqueous extract, which was the most molecularly different. Among those, two prostaglandins were identified (C 20 H 34 O 5 and C 20 H 34 O 6 ) with roles as an oxytocic, luteolytic, abortifacient, and vasodilator agents [ 69 , 70 ], together with a hydroxycoumarin (C 10 H 8 O 5 ) with multiple medical applications [ 71 , 72 , 73 ]. Multiple sesquiterpenes were also present with the molecular formulas C 24 H 34 O 10 , C 20 H 24 O 8 , C 19 H 28 O 6 , C 17 H 20 O 7 , C 15 H 18 O 4 , and C 15 H 18 O 3 , with a myriad of benefits, namely mycotoxic, anti-malarial, anti-diabetic, anti-inflammatory, anti-tumoral, anti-allergenic, anti-fungal, and anti-bacterial activity, among others [ 66 , 74 , 75 , 76 , 77 , 78 , 79 ].…”

Section: Resultsmentioning

confidence: 99%

Molecular Diversity and Biochemical Content in Two Invasive Alien Species: Looking for Chemical Similarities and Bioactivities

et al. 2022

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The biochemical composition, molecular diversity, and two different bioactivities of Asparagopsis armata and Rugulopteryx okamurae (two alien species with different invasive patterns in the southern Iberian Peninsula) were analyzed through spectrophotometric methods and Fourier transform ion cyclotron mass spectroscopy (FT-ICR-MS). A total of 3042 molecular formulas were identified from the different extracts. The dH2O extracts were the most molecularly different. A. armata presented the highest content of nitrogenous compounds (proteins, CHON) and sulphur content, whereas R. okamurae was rich in carbonated compounds (total carbon, lipids, CHO, and CHOP). Antioxidant capacity and phenolic content were higher in R. okamurae than in A. armata. Antimicrobial activity was detected from both species. A. armata showed capacity to inhibit human and fish pathogens (e.g., Staphylococcus aureus or Vibrio anguillarum), whereas R. okamurae only showed inhibition against human bacteria (Staphylococcus aureus and Cutibacterium acnes). In R. okamurae, molecules with a great number of pharmaceutical activities (e.g., anti-inflammatory or antitumoral), antibacterial, biomaterial, and other utilities were found. The main molecules of A. armata had also pharmaceutical applications (e.g., antimalarian, antithrombotic, anti-inflammatory, or antiarthritis). The valorization of these species can help to counteract the environmental effects of the bioinvasions.

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Fraxetin prevented sodium fluoride-induced chronic pancreatitis in rats: Role of anti-inflammatory, antioxidant, antifibrotic and anti-apoptotic activities

Cited by 15 publications

References 48 publications

An in‐silico investigation of fluoride ions impact on pancreatic lipase

An in‐silico investigation of fluoride ions impact on pancreatic lipase

Redox and biometal status in Wistar rats after subacute exposure to fluoride and selenium counter-effects

Molecular Diversity and Biochemical Content in Two Invasive Alien Species: Looking for Chemical Similarities and Bioactivities

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