Fraxinus mandshurica 4-coumarate-CoA ligase 2 enhances drought and osmotic stress tolerance of tobacco by increasing coniferyl alcohol content

Chen, Xiaohui; Su, Wenlong; Zhang, Han; Yu, Zhan; Zeng, Fansuo

doi:10.1016/j.plaphy.2020.08.031

Cited by 35 publications

(27 citation statements)

References 57 publications

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“…It can be speculated that they may be the most important regulated genes in response to sulfide stress in U. unicinctus ( Figure 6 ). These four genes have been reported as being involved in stress response and immune defense [ 62 , 63 , 64 , 65 ]. Here, we speculate that they may play important roles in the response to sulfide stress, and further studies are needed to clarify the potential molecular mechanisms.…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Analyses of Heat Shock Protein Superfamily Provide New Insights on Adaptation to Sulfide-Rich Environments in Urechis unicinctus (Annelida, Echiura)

Liu

Qin

Wei

et al. 2022

IJMS

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The intertidal zone is a transitional area of the land-sea continuum, in which physical and chemical properties vary during the tidal cycle and highly toxic sulfides are rich in sediments due to the dynamic regimes. As a typical species thriving in this habitat, Urechis unicinctus presents strong sulfide tolerance and is expected to be a model species for sulfide stress research. Heat shock proteins (HSPs) consist of a large group of highly conserved molecular chaperones, which play important roles in stress responses. In this study, we systematically analyzed the composition and expression of HSPs in U. unicinctus. A total of eighty-six HSP genes from seven families were identified, in which two families, including sHSP and HSP70, showed moderate expansion, and this variation may be related to the benthic habitat of the intertidal zone. Furthermore, expression analysis revealed that almost all the HSP genes in U. unicinctus were significantly induced under sulfide stress, suggesting that they may be involved in sulfide stress response. Weighted gene co-expression network analysis (WGCNA) showed that 12 HSPs, including 5 sHSP and 4 HSP70 family genes, were highly correlated with the sulfide stress response which was distributed in steelblue and green modules. Our data indicate that HSPs, especially sHSP and HSP70 families, may play significant roles in response to sulfide stress in U. unicinctus. This systematic analysis provides valuable information for further understanding of the function of the HSP gene family for sulfide adaptation in U. unicinctus and contributes a better understanding of the species adaptation strategies of marine benthos in the intertidal zone.

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Section: Resultsmentioning

confidence: 99%

Genome-Wide Analyses of Heat Shock Protein Superfamily Provide New Insights on Adaptation to Sulfide-Rich Environments in Urechis unicinctus (Annelida, Echiura)

Liu

Qin

Wei

et al. 2022

IJMS

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“…4-Coumarate-CoA ligase (4CL) is an important branch point involved in phenylpropanoid metabolism that regulates the biosynthesis of flavonoids, lignin and other phenolic secondary metabolites and plays important roles in plant physiology and biotic and abiotic stresses ( Lavhale et al, 2018 ; Chen et al, 2020 ; Wang et al, 2022 ). 4CL genes emerge in response to salt stress in a variety of plants.…”

Section: Discussionmentioning

confidence: 99%

“…These results indicated that ZmPDI may increase PFK4 expression to accelerate the conversion of F6P into F16BP, thereby enhancing the glycolysis pathway to maintain normal photosynthetic efficiency and chlorophyll content in zoysiagrass. 4-Coumarate-CoA ligase (4CL) is an important branch point involved in phenylpropanoid metabolism that regulates the biosynthesis of flavonoids, lignin and other phenolic secondary metabolites and plays important roles in plant physiology and biotic and abiotic stresses (Lavhale et al, 2018;Chen et al, 2020;Wang et al, 2022). 4CL genes emerge in response to salt stress in a variety of plants.…”

Section: Discussionmentioning

confidence: 99%

The combination of RNA-seq transcriptomics and data-independent acquisition proteomics reveals the mechanisms underlying enhanced salt tolerance by the ZmPDI gene in Zoysia matrella [L.] Merr.

Mei

Wang²,

Wang

et al. 2022

Front. Plant Sci.

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Zoysia matrella [L.] Merr. is one of the three most economically important Zoysia species due to its strong salt tolerance and wide application. However, the molecular mechanisms regulating salt tolerance in Z. matrella remain unknown. The protein disulfide isomerase ZmPDI of Z. matrella was obtained by salt stress screening with yeast cells, and its expression was significantly upregulated after salt stress. Based on the obtained ZmPDI overexpression transgenic Z. matrella plants, we carried out salt tolerance identification and found that ZmPDI can significantly enhance the salt tolerance of Z. matrella. Root samples of OX-ZmPDI transgenic and wild-type plants were collected at 0 and 24 h after salt treatments for RNA-seq and data-independent acquisition (DIA) proteome sequencing. Combined analysis of the transcriptome and proteome revealed that ZmPDI may enhance the salt tolerance of Z. matrella by regulating TUBB2, PXG4, PLDα2, PFK4, and 4CL1. This research presents the molecular regulatory mechanism of the ZmPDI gene in Z. matrella for resistance to salt stress and facilitates the use of molecular breeding to improve the salt tolerance of grasses.

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“…cDNA was diluted 10 times and used as the template. The data were normalized using the reference gene Fraxinus mandshurica α-tublin [ 1 , 51 ], Gene-specific primers for RT-qPCR analysis were listed in Additional file 1 . For fluorescence qPCR, a SYBR Green (TOYOBO) kit was used.…”

Section: Methodsmentioning

confidence: 99%

“…Plants have inherent properties that allow for a variety of physiological, cellular, and molecular responses to be involved throughout development and are susceptible to biotic and abiotic stresses [ 1 ]. These developmental processes have been suggested to be regulated by various transcription factors (TFs) [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis and molecular dissection of the SPL gene family in Fraxinus mandshurica

Gao

Han

et al. 2022

BMC Plant Biol

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Background SQUAMOSA promoter binding protein-like (SPL) is a unique family of transcription factors in plants, which is engaged in regulating plant growth and development, physiological and biochemical processes. Fraxinus mandshurica is an excellent timber species with a wide range of uses in northeastern China and enjoys a high reputation in the international market. SPL family analysis has been reported in some plants while SPL family analysis of Fraxinus mandshurica has not been reported. Results We used phylogeny, conserved motifs, gene structure, secondary structure prediction, miR156 binding sites, promoter cis elements and GO annotation to systematically analyze the FmSPLs family. This was followed by expression analysis by subcellular localization, expression patterns at various tissue sites, abiotic stress and hormone induction. Because FmSPL2 is highly expressed in flowers it was selected to describe the SPL gene family of Fraxinus mandshurica by ectopic expression. Among them, 10 FmSPL genes that were highly expressed at different loci were selected for expression analysis under abiotic stress (NaCl and Cold) and hormone induction (IAA and ABA). These 10 FmSPL genes showed corresponding trends in response to both abiotic stress and hormone induction. We showed that overexpression of FmSPL2 in transgenic Nicotiana tabacum L. resulted in taller plants, shorter root length, increased root number, rounded leaves, and earlier flowering time. Conclusions We identified 36 SPL genes, which were classified into seven subfamilies based on sequence analysis. FmSPL2 was selected for subsequent heterologous expression by analysis of expression patterns in various tissues and under abiotic stress and hormone induction, and significant phenotypic changes were observed in the transgenic Nicotiana tabacum L. These results provide insight into the evolutionary origin and biological significance of plant SPL. The aim of this study was to lay the foundation for the genetic improvement of Fraxinus mandshurica and the subsequent functional analysis of FmSPL2.

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Fraxinus mandshurica 4-coumarate-CoA ligase 2 enhances drought and osmotic stress tolerance of tobacco by increasing coniferyl alcohol content

Cited by 35 publications

References 57 publications

Genome-Wide Analyses of Heat Shock Protein Superfamily Provide New Insights on Adaptation to Sulfide-Rich Environments in Urechis unicinctus (Annelida, Echiura)

Genome-Wide Analyses of Heat Shock Protein Superfamily Provide New Insights on Adaptation to Sulfide-Rich Environments in Urechis unicinctus (Annelida, Echiura)

The combination of RNA-seq transcriptomics and data-independent acquisition proteomics reveals the mechanisms underlying enhanced salt tolerance by the ZmPDI gene in Zoysia matrella [L.] Merr.

Genome-wide analysis and molecular dissection of the SPL gene family in Fraxinus mandshurica

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