Frecuencia de los genotipos babA2, oipA y cagE de Helicobacter pylori en pacientes colombianos con enfermedades gastroduodenales.

Quiroga, Andrés Javier; Cittelly, Diana M.; Bravo, María Mercedes

doi:10.7705/biomedica.v25i3.1357

Cited by 25 publications

(20 citation statements)

References 37 publications

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“…Nevertheless, a recent study reported that babA2 prevalence was significantly higher in gastric biopsies obtained from chronic gastritis patients (95%) when compared with duodenal ulcer patients (18.1%) and non-ulcer dyspepsia subjects (26.1%) (30) . Arévalo et al found that 57% of the gastric isolates were babA2+ (3) , which coincides with other South American studies that reported gene frequencies ranging from 40.4% to 82.3% in stomach samples (16,27,29,37) . When babA2 and cagA are coexpressed in the same H. pylori strain, they work synergistically in worsening inflammation and may be a potential risk of intestinal metaplasia (30) .…”

Section: Discussionsupporting

confidence: 79%

Correlation between virulence markers of Helicobacter pylori in the oral cavity and gastric biopsies

Medina¹,

Medina

Merino

2017

Arq. Gastroenterol.

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-Background -The clinical outcome of Helicobacter pylori infection has been associated with virulence factors. The presence of these factors is useful as molecular markers in the identification of the high risk for developing severe gastric pathologies. Objective -To correlate the presence of virulence markers cagA and bab2A of H. pylori in oral and gastric biopsy samples. Methods -An observational, prospective, descriptive, and cross-sectional study was carried out between September 2011 and September 2012. Patients suffering dyspepsia with indication for upper gastrointestinal video endoscopy who attended the Gastroenterology Service of the Hospital Dr. Julio C. Perrando were included. Epidemiological investigation was completed. To detect the bacteria and their virulence genes, samples of saliva, dental plaque and gastric biopsy were taken and processed by PCR.Results -Sixty-one patients were selected for this study (30 women and 31 men). H. pylori was detected in 31 gastric biopsies and 31 oral samples.Significant difference between oral and gastric samples was found in cagA genotype. Agreement between oral and gastric genotypes was found in 38.7% of samples from the same patient. Conclusion -This study is the first in provide information about the genotypes of the Argentinean Northeast H. pylori strains. Despite the high prevalence of H. pylori infection, the most of patients had less virulent genotypes in oral cavity and gastric tissue. The cagA/babA2 combination was not frequent in the samples studied. There was not a statistical correlation between the virulence genes and gastroduodenal or oral diseases. Although in some patients the same genotype was found both in oral and gastric samples, it cannot be ensure that they corresponding to the same strain because a DNA sequencing was not performed.

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Section: Discussionsupporting

confidence: 79%

Correlation between virulence markers of Helicobacter pylori in the oral cavity and gastric biopsies

Medina¹,

Medina

Merino

2017

Arq. Gastroenterol.

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“…Los productos de la PCR se analizaron mediante electroforesis en geles de agarosa al 1,5 % y tinción con bromuro de etidio. Como controles se utilizaron la cepa de referencia NCTC11638 con genotipo positivo para vacA s1m1 cagA y la cepa 3062 con genotipo negativo para vacA s2m2 cagA obtenida en un estudio previo (16).…”

Section: Análisis De Vaca Y Caga Mediante Pcrunclassified

“…Aunque en Colombia la prevalencia de infección por H. pylori es alta en varias regiones (15,16), existen acentuadas diferencias en la incidencia de cáncer gástrico entre las zonas montañosas de los Andes, con tasas muy altas, y las regiones costeras, con tasas muy bajas (17,18). El propósito de este estudio fue determinar las frecuencias de los genotipos bacterianos cagA y vacA s1/s2 y m1/ m2 en dos regiones geográficas de Colombia con alta y baja incidencia de cáncer gástrico.…”

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Genotipificación de los factores de virulencia vacA y cagA de Helicobacter pylori en individuos de dos regiones de Colombia con riesgo opuesto de cáncer gástrico

2014

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“…In Colombia, there have been some studies reporting the frequency of vacA, cagA, and iceA, (Cittelly et al, 2002) babA2 and iceA, (Montealegre et al, 2009) alleles of vacA (López et al, 2009) or babA2, oipA and cagE (Quiroga et al, 2005). Considering that gastric cancer is a leading cause of death, our objective was to determine the association and prevalence of cagA, vacA, iceA and babA2 genes in patients with functional dyspepsia, due to the significance of these virulence factors in H. pylori pathogenicity (Atherton, 1998;Van Doorn et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of cagA, vacA, babA2 and iceA Genes in Helicobacter pylori Strains Isolated from Colombian Patients with Functional Dyspepsia

Arévalo-Galvis¹,

Trespalacios-Rangel²,

Otero³

et al. 2012

Pol J Microbiol

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The clinical outcome of Helicobacter pylori infection has been particularly associated with virulence genotypes. These genotypes are useful as molecular markers in the identification of patients that are infected and at high risk for developing more severe gastric pathologies. Our main objective was to determine the prevalence of virulence genotypes cagA, vacA, iceA and babA2 of H. pylori, in patients with functional dyspepsia who are infected with the bacteria. H. pylori genotypes babA2 and cagA as well as vacA and iceA allelic variants were identified by PCR in 122 isolates resulting from 79 patients with functional dyspepsia. A high prevalence of genes cagA+ (71%), vacAs1am1 (34%), babA2 (57%) and iceA1 (87%) was found. The most frequent combined genotype found were cagA+/vacAs1am1/babA2+/iceA1 and cagA-/vacAs1am1/babA2+/iceA1, regardless of any family history of gastric cancer or MALT lymphoma. The very virulent genotype cagA+/vacAs1am1/babA2+/iceA1 prevailed in the studied patients with functional dyspepsia. Our results provide information about the prevalence of four of the more important virulent factors and constitute new evidence on the prevalence of the most virulent H. pylori genotype in patients with functional dyspepsia.

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Frecuencia de los genotipos babA2, oipA y cagE de Helicobacter pylori en pacientes colombianos con enfermedades gastroduodenales.

Cited by 25 publications

References 37 publications

Correlation between virulence markers of Helicobacter pylori in the oral cavity and gastric biopsies

Correlation between virulence markers of Helicobacter pylori in the oral cavity and gastric biopsies

Genotipificación de los factores de virulencia vacA y cagA de Helicobacter pylori en individuos de dos regiones de Colombia con riesgo opuesto de cáncer gástrico

Prevalence of cagA, vacA, babA2 and iceA Genes in Helicobacter pylori Strains Isolated from Colombian Patients with Functional Dyspepsia

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