Free amino acid profiling in the giant puffball mushroom (Calvatia gigantea) using UPLC–MS/MS

Kıvrak, İbrahim; Kıvrak, Şeyda; Harmandar, Mansur

doi:10.1016/j.foodchem.2014.02.108

Cited by 61 publications

(40 citation statements)

References 23 publications

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“…The analytical column was a Waters ACQUITY UPLC BEH C18 column (2.1 mm × 50 30 mm, 1.7 μm) with a column temperature maintained at 35 °C. The analytical column was a Waters ACQUITY UPLC BEH C18 column (2.1 mm × 50 30 mm, 1.7 μm) with a column temperature maintained at 35 °C.…”

Section: Uplc-q-tof/ms Conditionsmentioning

confidence: 99%

“…The five subtypes exhibited the same structure of A ring at the aglycone part. The specific cracking process for luteolin-7-o- 30 glucoside is shown in Figure. 45 According to their chemical compositions, the organic acids in the KDZ injection could be classified as simple organic acids and acylated organic acids. After the components were classified and identified, compounds 26-33 were obtained as flavonoids (the specific information are shown in Table 2).…”

Section: Flavonoidsmentioning

confidence: 99%

“…Based on their structure, 55 acylated organic acids were divided into six types: caffeoyltartaric acid (CTA, type A), dicaffeoyltartaric acid (DTA, type B), caffeoylquinic acid (CQA, type C), dicaffeoylquinic acid (DiCQA, type D), ferulylquinic acid (FQA, type E) and cinnamoylquinic acid (CiQA, type F). 30 Thus, this characteristic of these compounds was considered in MS to classify and identify the amino acids. After classification and identification were performed, compounds 10-25 were obtained as organic acids (the specific details are shown in Table 2).…”

Section: Flavonoidsmentioning

confidence: 99%

“…The 90 specific cracking process for 3-CQA is shown in Figure. fragments; thus, the precise structure should be determined on the basis of the relative abundance of fragments. 30 Table 1. Conversely, 4-CQA exhibits a base peak at m/z 173, and this finding is 105 consistent with that in a previous study.…”

Section: Flavonoidsmentioning

confidence: 99%

“…It helps to improve microcirculation and anti- 25 platelet aggregation, lowers blood pressure and treats coronary heart disease, angina, infarction and other clinical diseases. 1-2 Although KDZ injection is a single-herb preparation, its compositions are complicated; the main components of KDZ are nucleosides, organic acids, flavonoids, amino acids and other 30 compounds. [3][4] Liquid chromatography-mass spectrometry (LC-MS) has been widely applied to investigate the components of single-herb and complex preparations with its advantages including high separation, high selectivity and good sensitivity.…”

Section: Introductionmentioning

confidence: 99%

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Rapid classification and identification of complex chemical compositions in traditional Chinese medicine based on UPLC-Q-TOF/MS coupled with data processing techniques using the KuDieZi injection as an example

et al. 2015

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KuDieZi (KDZ) injection is prepared by extracting and processing Ixeris sonchifolia [Ixeris sonchifolia Hance] belonging to Lactuca genus of the Asteraceae family. KDZ injection is a single-herb preparation in which components are analysed by liquid chromatographymass spectrometry. However, the chemical compositions of this preparation are complex and diverse. Hence, data processing is complicated and time consuming; furthermore, data processing can not provide a systematic, accurate and repeatable method to rapidly 10 classify and identify chemical constituents of the injection. In our study, the main components, particularly flavonoids, organic acids, amino acids and nucleosides in KDZ injection, were rapidly classified and identified by data processing technology based on UPLC-Q-TOF/MS. After we reviewed lots of studies and collected the fragments' information, then compared with the mass spectrometric analyses in standards, the rules of diagnostic fragments (DFs) and neutral losses (NLs) of the four substances were established and summarised. A rapid classification and identification method of the chemical compositions of KDZ injection was then constructed using 15 DF filter (DFF) and NL filter (NLF). This method was applied to analyse the KDZ injection. A total of 31 chemical components, which included 8 flavonoids, 13 organic acids, 6 amino acids and 4 nucleosides, were obtained. DFF and NLF were used to rapidly classify and identify chemical substances in KDZ fingerprint. With this method, we effectively solved the technical difficulties in fingerprint resolution caused by complex components and low levels in traditional Chinese medicine (TCM). In addition, this study provided a novel approach for further studies on TCM.

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Section: Uplc-q-tof/ms Conditionsmentioning

confidence: 99%

Section: Flavonoidsmentioning

confidence: 99%

Section: Flavonoidsmentioning

confidence: 99%

Section: Flavonoidsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rapid classification and identification of complex chemical compositions in traditional Chinese medicine based on UPLC-Q-TOF/MS coupled with data processing techniques using the KuDieZi injection as an example

et al. 2015

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Chemical, Nutritional, and Bioactive Potential of Mushrooms

Fernandes

Heleno

2017

Edible and Medicinal Mushrooms

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Simultaneous determination and qualitative analysis of six types of components in Naoxintong capsule by miniaturized matrix solid‐phase dispersion extraction coupled with ultra high‐performance liquid chromatography with photodiode array detection and quadrupole time‐of‐flight mass spectrometry

Wang

Jiang

Ding

et al. 2018

J of Separation Science

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A simple and effective sample preparation process based on miniaturized matrix solid-phase dispersion was developed for simultaneous determination of phenolic acids (gallic acid, chlorogenic acid, ferulic acid, 3,5-dicaffeoylqunic acid, 1,5-dicaffeoylqunic acid, rosmarinic acid, lithospermic acid, and salvianolic acid B), flavonoids (kaempferol-3-O-rutinoside, calycosin, and formononetin), lactones (ligustilide and butyllidephthalide), monoterpenoids (paeoniflorin), phenanthraquinones (cryptotanshinone), and furans (5-hydroxymethylfurfural) in Naoxintong capsule by ultra high-performance liquid chromatography. The optimized condition was that 25 mg Naoxintong powder was blended homogeneously with 100 mg Florisil PR for 4 min. One milliliter of methanol/water (75:25, v/v) acidified by 0.05% formic acid was selected to elute all components. It was found that the recoveries of the six types of components ranged from 61.36 to 96.94%. The proposed miniaturized matrix solid-phase dispersion coupled with ultra high-performance liquid chromatography was successfully applied to simultaneous determination of the six types of components in Naoxintong capsules. The results demonstrated that the proposed miniaturized matrix solid-phase dispersion coupled with ultra high-performance liquid chromatography could be used as an environmentally friendly tool for the extraction and determination of multiple bioactive components in natural products.

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Free amino acid profiling in the giant puffball mushroom (Calvatia gigantea) using UPLC–MS/MS

Cited by 61 publications

References 23 publications

Rapid classification and identification of complex chemical compositions in traditional Chinese medicine based on UPLC-Q-TOF/MS coupled with data processing techniques using the KuDieZi injection as an example

Rapid classification and identification of complex chemical compositions in traditional Chinese medicine based on UPLC-Q-TOF/MS coupled with data processing techniques using the KuDieZi injection as an example

Chemical, Nutritional, and Bioactive Potential of Mushrooms

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