Free ammonia inhibition in microalgae and cyanobacteria grown in wastewaters: Photo-respirometric evaluation and modelling

Rossi, S.; Díez-Montero, Rubén; Rueda, Estel; Cascino, Federico Castillo; Parati, Katia; García, Joan; Ficara, Elena

doi:10.1016/j.biortech.2020.123046

Cited by 69 publications

(25 citation statements)

References 37 publications

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“…The tests described in this work reached fairly high ammonia concentrations, which might explain the photosynthetic inhibitory effect. Rossi et al ( 2020 ) used photo-respirometric tests to determine the EC 50,NH3 , which represents the free ammonia concentration causing a 50% inhibition of photosynthetic activity in a microalgae monoculture. They evaluated two Scenedesmus strains, S.quadricuada and S.obliquus , which showed an EC 50,NH3 of 77.7 and 52.6 mgNH 3 ·L −1 , respectively (Rossi et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Rossi et al ( 2020 ) used photo-respirometric tests to determine the EC 50,NH3 , which represents the free ammonia concentration causing a 50% inhibition of photosynthetic activity in a microalgae monoculture. They evaluated two Scenedesmus strains, S.quadricuada and S.obliquus , which showed an EC 50,NH3 of 77.7 and 52.6 mgNH 3 ·L −1 , respectively (Rossi et al 2020 ). At these concentrations, S. almeriensis showed a reduction in net photosynthesis of 20% and 10%, respectively, lower than that described for the other strains.…”

Section: Discussionmentioning

confidence: 99%

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Modelling of photosynthesis, respiration, and nutrient yield coefficients in Scenedemus almeriensis culture as a function of nitrogen and phosphorus

Zurano

Gómez-Serrano

Acién-Fernández

et al. 2021

Appl Microbiol Biotechnol

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Photo-respirometric tecniques are applied for evaluating photosynthetic activity in phototrophic organisms. These methods allow to evaluate photosynthetic response under different conditions. In this work, the influence of nutrient availability (nitrate, ammonium, and phosphate) on the photosynthesis and respiration of Scenedesmus almeriensis was studied using short photo-respirometric measurements. Both photosynthesis and respiration increasing until saturation value and consecutively diminishing, presenting inhibition by high concentrations. Regarding the influence of phosphorus concentration in microalgae cells, a similar hyperbolic trend was observed but no inhibition was observed at high concentration. Based on these experimental data, the respiration, and the photosynthesis rate of S. almeriensis were modelled using Haldane equation for nitrate and ammonium data, and Monod equation for phosphate data. In addition, experiments were performed to determine the yield coefficients for both nitrogen and phosphorus in S. almeriensis cultures. The data showed that the nitrogen and phosphorous coefficient yields are not constant, being modified as a function of nutrients concentration, presenting the luxury uptake phenomena. Finally, the proposed models were incorporated into a simulation tool to evaluate the photosynthetic activity and the nutrient yield coefficients of S. almeriensis when different culture media and wastewaters are used as a nitrogen and phosphorous source for its growth.Key points• Microalgal photosynthesis/respiration vary as a function of nutrients availability.• Photosynthesis inhibition appears at high N-NO3-and N-NH4+concentrations.• Nutrient yield coefficients are influenced by luxury uptake phenomenon. Graphical abstract

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Modelling of photosynthesis, respiration, and nutrient yield coefficients in Scenedemus almeriensis culture as a function of nitrogen and phosphorus

Zurano

Gómez-Serrano

Acién-Fernández

et al. 2021

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

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“…Such concentrations could be inhibitory to microalgae growth, since at pH 9.2 and above, free ammonia become the dominant species. Although C. sorokiniana can tolerate ammonia concentration up to 96.3 mg.L −1 NH 3 (Rossi et al, 2020), this would in any case exclude a N limitation strategy for lipid accumulation. The effect of high NH 4 concentration on the mixotrophic growth of microalgae on VFA, out of the scope of the current study, should be thus further evaluated.…”

Section: Biomass Lipids and Carbohydrates Production Potential Of C Sorokinianamentioning

confidence: 99%

Mixotrophic Growth of Chlorella sorokiniana on Acetate and Butyrate: Interplay Between Substrate, C:N Ratio and pH

et al. 2021

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Microalgae can be cultivated on waste dark fermentation effluents containing volatile fatty acids (VFA) such as acetate or butyrate. These VFA can however inhibit microalgae growth at concentrations above 0.5-1 gC.L–1. This study used the model strain Chlorella sorokiniana to investigate the effects of acetate or butyrate concentration on biomass growth rates and yields alongside C:N:P ratios and pH control. Decreasing undissociated acid levels by raising the initial pH to 8.0 allowed growth without inhibition up to 5 gC.L–1 VFAs. However, VFA concentration strongly affected biomass yields irrespective of pH control or C:N:P ratios. Biomass yields on 1.0 gC.L–1 acetate were around 1.3-1.5 gC.gC–1 but decreased by 26-48% when increasing initial acetate to 2.0 gC.L–1. This was also observed for butyrate with yields decreasing up to 25%. This decrease in yield in suggested to be due to the prevalence of heterotrophic metabolism at high organic acid concentration, which reduced the amount of carbon fixed by autotrophy. Finally, the effects of C:N:P on biomass, lipids and carbohydrates production dynamics were assessed using a mixture of both substrates. In nutrient replete conditions, C. sorokiniana accumulated up to 20.5% carbohydrates and 16.4% lipids while nutrient limitation triggered carbohydrates accumulation up to 45.3%.

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“…It must be noted that pure cultures can only be cultivated in highly controlled lab‐scale conditions (Gao et al, 2016; Luo, Le‐Clech, & Henderson, 2018), while outdoors, contamination of microalgae with other microorganisms is expected (Galès et al, 2019; Shahid et al, 2020). These polycultures can increase microalgae productivity due to their better adaptability to variable conditions and their higher robustness, resistance, and efficiency in the use of resources (AlMomani & Örmeci, 2016; Rossi et al, 2020). For these reasons, mixed cultivation is the only feasible option for outdoor microalgae cultivation.…”

Section: Microalgae Cultivationmentioning

confidence: 99%

Outdoor microalgae‐based urban wastewater treatment: Recent advances, applications, and future perspectives

et al. 2021

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Although microalgae‐based wastewater treatment has been traditionally carried out in extensive waste stabilization ponds, recent trends focus on the use of microalgae to apply the circular economy principles in the wastewater treatment sector due to the capacity of algae to absorb carbon dioxide while recovering nutrients from sewage. To this aim, the development of new intensive microalgae‐based systems with higher efficiency and level of process control is required. Results obtained for these systems at lab scale are generally promising. However, upscaling to outdoor conditions is often uncertain. Some advances have been made in terms of applying open systems at large scale. However, there are still some issues related to land requirements and the economic feasibility and robustness of the process that have to be overcome to widely implement these systems. This article aims at describing the main design and operating factors regarding outdoor microalgae cultivation. It will also explain some microalgae cultivation technologies to treat wastewater, showing their advantages, disadvantages, and the possibility to treat different wastewater streams with microalgae cultures. Future perspectives of this biotechnology will be commented as well. This article is categorized under: Engineering Water > Engineering Water

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Free ammonia inhibition in microalgae and cyanobacteria grown in wastewaters: Photo-respirometric evaluation and modelling

Cited by 69 publications

References 37 publications

Modelling of photosynthesis, respiration, and nutrient yield coefficients in Scenedemus almeriensis culture as a function of nitrogen and phosphorus

Modelling of photosynthesis, respiration, and nutrient yield coefficients in Scenedemus almeriensis culture as a function of nitrogen and phosphorus

Mixotrophic Growth of Chlorella sorokiniana on Acetate and Butyrate: Interplay Between Substrate, C:N Ratio and pH

Outdoor microalgae‐based urban wastewater treatment: Recent advances, applications, and future perspectives

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