Free and Bound Sialic Acid in Rat and Hamster Epididymal Fluid/Freie und gebundene Sialinsäure in der Nebenhodenflüssigkeit der Ratte und des Hamsters

Verawatnapakul, V.; Pholpramool, Chumpol

doi:10.1111/j.1439-0272.1988.tb00709.x

Cited by 4 publications

(1 citation statement)

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“…The epididymal fluid and secretions contain substantial amounts of free and bound sialic acid [103][104][105], important components of the epididymal microenvironment [106]. The epididymis is a slender duct that can be further divided into caput, corpus, and cauda [35].…”

Section: Changes Of Sialylation During Sperm Maturationmentioning

confidence: 99%

Sialylation: fate decision of mammalian sperm development, fertilization, and male fertility

Feng

Wang

et al. 2023

Biology of Reproduction

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Sperm development, maturation, and successful fertilization within the female reproductive tract are intricate and orderly processes that involve protein translation and post-translational modifications. Among these modifications, sialylation plays a crucial role. Any disruptions occurring throughout the sperm’s life cycle can result in male infertility, yet our current understanding of this process remains limited. Conventional semen analysis often fails to diagnose some infertility cases associated with sperm sialylation, emphasizing the need to comprehend and investigate the characteristics of sperm sialylation. This review re-analyzes the significance of sialylation in sperm development and fertilization, and evaluates the impact of sialylation damage on male fertility under pathological conditions. Sialylation serves a vital role in the life journey of sperm, providing negatively charged glycocalyx and enriching the molecular structure of the sperm surface, which is beneficial to sperm reversible recognition and immune interaction. These characteristics are particularly crucial during sperm maturation and fertilization within the female reproductive tract. Moreover, enhancing the understanding of the mechanism underlying sperm sialylation can promote the development of relevant clinical indicators for infertility detection and treatment.

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Section: Changes Of Sialylation During Sperm Maturationmentioning

confidence: 99%

Sialylation: fate decision of mammalian sperm development, fertilization, and male fertility

Feng

Wang

et al. 2023

Biology of Reproduction

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The Testicular and Epididymal Luminal Amino Acid Microenvironment in the Rat

HINTON

1990

Journal of Andrology

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Concentrations of amino acids were measured in arterial and testicular venous blood, and in fluids from the seminiferous tubule, rete testis, and the caput, corpus, and cauda epididymidis. There were no significant differences in the concentrations of any amino acids between arterial and testicular venous blood, whereas there were significant differences between arterial/venous blood and testicular interstitial fluid. The predominant amino acids measured within seminiferous tubule fluid (STF) and rete testis fluid (RTF) were glycine, alanine, glutamate, and glutamine. RTF contained approximately equal concentrations of basic and total amino acids, but 17 times higher acidic amino acids and 1.2 and 1.3 times lower uncharged polar and nonpolar amino acids, respectively, compared to STF. The concentration of total amino acids within caput fluid reached over 50 mmol/L, but then declined to approximately 50% and 0.1% of caput for corpus and cauda, respectively. The predominant amino acids measured within epididymal luminal fluids were glutamate and taurine; glutamate contributed to ∼90% of the total amino acids measured in caput fluid. The presence of glutamate and taurine within the epididymal lumen is due primarily to a direct contribution from the epididymal epithelium, as measured using the split‐drop stopped‐flow microperfusion technique. Several other amino acids within the lumen also originate from the epididymal epithelium. Amino acids contribute approximately 20%, 9%, and 2% of the total osmolality of caput, corpus, and cauda fluid, respectively. Even when all ions and organic solutes are measured and compared to the total osmolality of luminal fluid within each epididymal region, there is still a difference of approximately 10, 70, and 35 mOsm/kg water for caput, corpus, and cauda, respectively.

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