Free and Conjugated Estrogens of an Ovarian Cyst adenoma and Granulosa Cell Tumor

Flickinger, George L.; Murawec, Taras; Touchstone, Joseph C.

doi:10.1210/jcem-25-9-1231

Cited by 8 publications

(3 citation statements)

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“…Formation of small quantities of oestrogen sulphates has been observed in tissue extracts of bovine ovaries by Holcenberg «fe Rosen (1965) and Payne & Mason (1965), but the ability of the human ovary to produce steroid sulphates has, until recently, been a point of controversy. Small amounts of dehydroepiandrosterone (DHEA) and oestrone sulphates were initially detected in human ovarian tumours and cyst fluid (Flickinger, Murawec & Touchstone, 1965; Giorgi, 1967) and later in cystic and follicle-stimu¬ lating hormone (FSH)-treated ovaries (Aakvaag «fe Fylling, 1968). Formation of trace amounts of the sulphates of DHEA and 5-androstene-3ß,17ß-diol by homogenates of human ovaries was observed by Wallace & Silberman (1964), and Melville & Braunsberg (1973) demonstrated the formation of the sulphates of DHEA and oestradiol in super¬ natant fractions of tissue from seven out of 12 human ovaries.…”

Section: Introductionmentioning

confidence: 99%

Intra-Ovarian Formation of Steroid Sulphates: Measurement and Influence of Gonadotrophins

Sturm¹,

Hannappel²

1978

Journal of Endocrinology

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The steroid sulphokinase activity in 105 000 g supernatant fractions from rat and bovine ovarian tissue has been determined by measuring the radioactivity incorporated into conjugated steroids after incubation with 14C-labelled androgens and oestrogens. The enzyme from bovine ovaries was partially characterized, changes in its activity with pH were measured and Km values of 30, 10 and 4 mumol/1 were obtained for testosterone, oestradiol and oestrone respectively. The rate of formation of sulphates from oestrogens by preparations of bovine luteal tissue was 10--20 times higher than the rate of formation by follicular tissue (P less than 0.001), but both tissues exhibited similar rate of sulphate formation with testosterone. Sulphation of dehydroepiandrosterone by the ovaries of gonadotrophin-treated rats did not differ from that observed in the control group, whereas sulphation of oestrone was nearly three times greater in ovaries obtained from rats treated with gonadotrophins (P less than 0.005). Furthermore, a comparison of steriod sulphation in the ovaries of pseudopregnant and normal cyclic rats showed that the sulphation of oestrone was about six times greater during pseudopregnancy. These observations suggest that the formation of oestrogen, but not androgen, sulphates by ovarian aryl sulphokinase differs in follicular and luteal tissue and may be gonadotrophin dependent. The greater capacity of luteal, compared with follicular, tissue for the sulphation of oestrogens may be a control mechanism for steroidogenesis within the ovarian tissue.

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Section: Introductionmentioning

confidence: 99%

Intra-Ovarian Formation of Steroid Sulphates: Measurement and Influence of Gonadotrophins

Sturm¹,

Hannappel²

1978

Journal of Endocrinology

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“…The 60% o f oestrone that was not produced from plasma androstenedione presumably originated from direct secretion or by peripheral conversion of other oestrogens secreted b y the thecoma, or both. Although the most likely plasma oestrogen t o be converted to oestrone is unconjugated oestradiol, iri vitro studies have shown that ovarian tuniours may produce oestrone sulphate (Flickinger et al, 1965) which may be an alternative source of plasma oestrone (Longcope, 1971). However, total urinary oestrogen levels were within the normal ranges for postmenopausal women (Brown et al, 1968) so it is unlikely that the amount of oestrone sulphate secreted by the thecoma exceeded that o f unconjugated oestradiol.…”

Section: Discussionmentioning

confidence: 99%

Plasma Hormone Levels and Oestrogen Production in a Postmenopausal Woman With Endometrial Carcinoma and an Ovarian Thecoma

Reed

Hutton

Beard

et al. 1979

Clinical Endocrinology

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SUMMARY Plasma hormone concentrations, the production rate of oestrone from androstenedione, and the total production rate of oestrone have been measured in a 57‐year‐old woman with an ovarian thecoma and endometrial carcinoma before and 2 months after hysterectomy and bilateral ovariectomy. The plasma concentration of oestradiol (184 pmol/l), but not oestrone (144 pmol/l), was elevated pre‐operatively and FSH secretion was suppressed (115.0 μg/l). Post‐operatively the plasma concentration of oestradiol was significantly reduced (80 pmol/l) and the concentration of FSH (571 μg/l) had increased to a postmenopausal level. The concentration of oestradiol in samples of plasma obtained simultaneously at operation from the ovarian vein of the ovary containing the thecoma (3.38 nmol/l) was fifteen times greater than in the periphery (228.0 pmol/l), and this confirmed that oestradiol was secreted by the thecoma. The total production rates of oestrone pre‐and post‐operatively were 101.3 nmo1/24 h and 29.2 nmo1/24 h respectively and the production rates of oestrone from androstenedione were 41.4 nmo1/24 h and 32.9 nmo1/24 h. Thus, postaperatively, all the oestrone was produced from circulating androstenedione, whereas pre‐operatively only 40% of the oestrone was produced by the extraglandular conversion of androstenedione; the remaining 60% presumably originated from the peripheral conversion of oestradiol secreted by the thecoma. It is concluded that the oestradiol secreted by the thecoma was probably the major factor which caused the development of endometrial carcinoma in this subject.

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“…On the other hand, estradiol substantially increases the capacities of plasma cortisol‐binding globulin and thyroxine‐binding globulin, whereas estriol (20–40 mg daily for seven to fourteen days) does not significantly increase these capacities (88). The presence of high concentrations of estrone in cystadenoma and granulosa‐cell tumor of the ovary (87f) may signify the possible oncogenic role of this hormone.…”

Section: Possible Mechanisms Of Estrogenic Actionmentioning

confidence: 99%

Estrogenic Management of Benign Prostatism, Including Early and Poor‐risk Cases: 7‐year Experience*

Roberts

1966

J American Geriatrics Society

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Free and Conjugated Estrogens of an Ovarian Cyst adenoma and Granulosa Cell Tumor

Cited by 8 publications

References 0 publications

Intra-Ovarian Formation of Steroid Sulphates: Measurement and Influence of Gonadotrophins

Intra-Ovarian Formation of Steroid Sulphates: Measurement and Influence of Gonadotrophins

Plasma Hormone Levels and Oestrogen Production in a Postmenopausal Woman With Endometrial Carcinoma and an Ovarian Thecoma

Estrogenic Management of Benign Prostatism, Including Early and Poor‐risk Cases: 7‐year Experience*

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