FREE CULTURE MEDIA OF GROWTH REGULATORS ON MICROPROPAGATION OF GRAPEVINE (Vitis labrusca L.) ‘BORDÔ’ CULTIVAR THROUGH NODAL SEGMENTS

Abstract

The micropropagation is an important biotechnological tool for obtaining and maintaining mother grapevine plants with high quality plant health. The objective was to evaluate the establishment and multiplication in vitro and ex vitro acclimatization of grapevine cultivate Bordô in different culture media without adding growth regulators. Grapevine nodal segments were cultured in five culture media formulations without adding growth regulators. It was evaluated the number of leaves and roots, length of roots and shoots, replication rate, relative chlorophyll index, percentage of regenerated and rooted plants, dry biomass of shoot, root and total plants grown in vitro and after acclimatization. In vitro propagation of grapevine Bordô through nodal segments provided high rates of regeneration and rooting. High survival rates were obtained in the acclimatization of Bordô. Considering all the varia-______________

In Vitro Collection for the Safe Storage of Grapevine Hybrids and Identification of the Presence of Plasmopara viticola Resistance Genes

“…The effectiveness of a propagation protocol depends largely on the rate of shoot multiplication [ 52 ]. The shoot multiplication rate (MR) reflects the number of new progeny plants produced from a single explant [ 56 , 72 ]. We found that the concentration of phytohormones and the acidity of the culture medium have a great effect on the MR and the quality of in vitro stock cultures ( Table 4 ).…”