Free-floating adult human brain-derived slice cultures as a model to study the neuronal impact of Alzheimer’s disease-associated Aβ oligomers

Mendes, Niele Dias; Fernandes, Artur José de Brum; Almeida, Gláucia Maria; Santos, Luís Eduardo; Selles, Maria Clara; Silva, N.M. Lyra e; Machado, Carla M.; Horta-Júnior, J. A. C.; Louzada, Paulo Roberto; Felice, Fernanda G. De; Alves-Leon, Soniza Vieira; Marcondes, Jorge; Assirati, João Alberto; Matias, Caio; Klein, William L.; García-Cairasco, Norberto; Ferreira, Sérgio T.; Neder, Luciano; Sebollela, Adriano

doi:10.1016/j.jneumeth.2018.05.021

Cited by 28 publications

(46 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The in vitro spontaneous interictal-like activity recorded in the subiculum of human acute temporal lobe slice cultures appears to resemble intracranial EEG recordings from the same patient with temporal lobe epilepsy [44]. Human temporal cortical slices exposed to amyloid-beta oligomers (AbOs) present robust binding of AbOs and elevated levels of hyperphosphorylated Tau, the second hallmark of AD [45]. In addition, hippocampal slices from patients who underwent surgical removal of epileptic foci have been maintained successfully in culture for up to 25 days, and the cell viability was [ 50%.…”

Section: Human Brain Slice Culturementioning

confidence: 99%

Human Brain Slice Culture: A Useful Tool to Study Brain Disorders and Potential Therapeutic Compounds

Verwer

Bao

et al. 2019

Neurosci. Bull.

View full text Add to dashboard Cite

Investigating the pathophysiological mechanisms underlying brain disorders is a priority if novel therapeutic strategies are to be developed. In vivo studies of animal models and in vitro studies of cell lines/primary cell cultures may provide useful tools to study certain aspects of brain disorders. However, discrepancies among these studies or unsuccessful translation from animal/cell studies to human/clinical studies often occur, because these models generally represent only some symptoms of a neuropsychiatric disorder rather than the complete disorder. Human brain slice cultures from postmortem tissue or resected tissue from operations have shown that, in vitro, neurons and glia can stay alive for long periods of time, while their morphological and physiological characteristics, and their ability to respond to experimental manipulations are maintained. Human brain slices can thus provide a close representation of neuronal networks in vivo, be a valuable tool for investigation of the basis of neuropsychiatric disorders, and provide a platform for the evaluation of novel pharmacological treatments of human brain diseases. A brain bank needs to provide the necessary infrastructure to bring together donors, hospitals, and researchers who want to investigate human brain slices in cultures of clinically and neuropathologically well-documented material. Keywords Alzheimer's disease Á Brain bank Á Brainderived neurotrophic factor Á Depression Á Electrical activity Á Human brain slice culture Á Neuropsychiatric disorders Á Organotypic culture Á Postmortem human brain tissue Á Resected human brain tissue Xin-Rui Qi and Ronald W. H. Verwer have contributed equally to this review.

show abstract

Section: Human Brain Slice Culturementioning

confidence: 99%

Human Brain Slice Culture: A Useful Tool to Study Brain Disorders and Potential Therapeutic Compounds

Verwer

Bao

et al. 2019

Neurosci. Bull.

View full text Add to dashboard Cite

show abstract

“…Brain slice culture can successfully simulate the process of neural development similar to that observed in vivo, and provide experimental evidence for organ‐like brain slices, as an alternative to the in vivo brain . This method has been effectively applied to various fields of experimental neuroscience …”

Section: Introductionmentioning

confidence: 99%

“…5 This method has been effectively applied to various fields of experimental neuroscience. [6][7][8][9] Today, organotypic brain slice culture is mainly applied to mouse and rat tissue, as well as for disease modelling, such as with transgenic mice. [10][11][12][13][14][15][16][17] However, there are few reports related to the study of organotypic slice culture using chicken embryos.…”

Section: Introductionmentioning

confidence: 99%

Organotypic slice culture based on in ovo electroporation for chicken embryonic central nervous system

Yang

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

Organotypic slice culture is a living cell research technique which blends features of both in vivo and in vitro techniques. While organotypic brain slice culture techniques have been well established in rodents, there are few reports on the study of organotypic slice culture, especially of the central nervous system (CNS), in chicken embryos. We established a combined in ovo electroporation and organotypic slice culture method to study exogenous genes functions in the CNS during chicken embryo development. We performed in ovo electroporation in the spinal cord or optic tectum prior to slice culture. When embryonic development reached a specific stage, green fluorescent protein (GFP)‐positive embryos were selected and fluorescent expression sites were cut under stereo fluorescence microscopy. Selected tissues were embedded in 4% agar. Tissues were sectioned on a vibratory microtome and 300 μm thick sections were mounted on a membrane of millicell cell culture insert. The insert was placed in a 30‐mm culture dish and 1 ml of slice culture media was added. We show that during serum‐free medium culture, the slice loses its original structure and propensity to be strictly regulated, which are the characteristics of the CNS. However, after adding serum, the histological structure of cultured‐tissue slices was able to be well maintained and neuronal axons were significantly longer than that those of serum‐free medium cultured‐tissue slices. As the structure of a complete single neuron can be observed from a slice culture, this is a suitable way of studying single neuronal dynamics. As such, we present an effective method to study axon formation and migration of single neurons in vitro.

show abstract

“…O modelo de fatias cerebrais humanas já foi padronizado por nosso grupo em trabalhos anteriores, nos quais mostramos que estas se encontram viáveis e funcionais por até 5DIV, com a preservação da citoarquitetura do tecido neural, e com a presença dos principais tipos celulares: neurônios, micróglia e astrócitos (Fig. 2) (Mendes et al, 2018;Fernandes et al, 2019), características importantes para que o modelo possa ser utilizado em estudos de neuropatogênese viral.…”

Section: Discussão 5) Discussãounclassified

“…A utilização de tecido cerebral humano obtido por ressecções cirúrgicas para preparo de culturas de fatias tem ganhado força nos últimos anos. Na maior parte dos estudos, o tecido cerebral é obtido da retirada de tumores ou de foco epilético em pacientes com epilepsia do lobo temporal (ELT) resistente ao tratamento farmacológico (Brewer et al, 2001;Sebollela et al, 2012;Verhoog et al, 2013;Eugène et al, 2014;Mendes et al, 2018). Nesse procedimento, pacientes com ELT refratários a tratamento farmacológico são submetidos a remoção cirúrgica do foco epilético que, nesses casos, se encontra no hipocampo.…”

Section: Introdução 1) Introduçãounclassified

Estudo da infecção do sistema nervoso central pelo vírus Oropouche em um modelo de fatias de cérebro humano adulto

Almeida¹

View full text Add to dashboard Cite

Almeida, Glaucia Maria. Study of central nervous system infection by the Oropouche vírus in adult human brain slice cultures. Ribeirão Preto, 2020. The Oropouche virus (OROV), of the family Peribunyaviridae, genus Orthobunyavirus, is a causative agent of arbovirus called "Oropouche fever". This virus has the significant spreading capacity, and in recent years has been found in nonendemic region, especially in South American countries, including Brazil. Although some clinical evidences in alterations in central nervous system, as meningitis, has been associated with OROV infection, few studies involving OROV neuropathogenesis have been studied, and all knowledge obtained were showed from neonatal murine models. This difficulty in studying OROV neuropathogenesis in the human central nervous system is related to the lack of models that maintain the complexity of human brain. Therefore, in this study, we evaluated the OROV neuroinfection using adult human brain slices cultures. We showed for the first time that OROV infects human neural cells and caused tissue damage within 48 h post infection, such as loss of cell viability and TNF-α release, a proinflammatory cytokine. We showed, by immunofluorescence, microglia cells were the cell most susceptible to OROV infection, but that it retains the ability to infect neurons. This is the first study to report OROV infection in adult human brain model and should become useful in neuropathogenesis studies related to this emerging pathogen.

show abstract

Free-floating adult human brain-derived slice cultures as a model to study the neuronal impact of Alzheimer’s disease-associated Aβ oligomers

Cited by 28 publications

References 62 publications

Human Brain Slice Culture: A Useful Tool to Study Brain Disorders and Potential Therapeutic Compounds

Human Brain Slice Culture: A Useful Tool to Study Brain Disorders and Potential Therapeutic Compounds

Organotypic slice culture based on in ovo electroporation for chicken embryonic central nervous system

Estudo da infecção do sistema nervoso central pelo vírus Oropouche em um modelo de fatias de cérebro humano adulto

Contact Info

Product

Resources

About