Free-Living Enterobacterium <i>Pragia fontium</i> 24613: Complete Genome Sequence and Metabolic Profiling

Snopková, Kateřina; Sedlář, Karel; Bosák, Juraj; Chaloupková, Eva; Sedláček, Ivo; Provazník, Ivo; Šmajs, David

doi:10.1177/1176934317700863

Cited by 3 publications

(3 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…and Leminorella spp., and it contains only one species, P. fontium, a free-living bacterium isolated from an environment that under anaerobic conditions utilizes monosaccharides and their derivatives but lacks fatty acid degradation pathways (Snopková et al 2017). The genus Budvicia has been identified in the red palm weevil (Tagliavia et al 2014), while Pragia was previously found to be associated with the gut of Poecilus chalcites (Lehman et al 2009).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Gut microbial community response to herbicide exposure in a ground beetle

Giglio

Vommaro

Gionechetti

et al. 2021

J Applied Entomology

View full text Add to dashboard Cite

Gut microbiota plays a key role in physiological processes of insects, including nutritional metabolism, development, immunity and detoxification. Environmental stressors such as herbicides, used to optimize and improve crop yields, may interfere with the mutualistic relationships causing negative consequences for the host health. Dinitroaniline herbicides, for example pendimethalin, are used worldwide in pre‐emergence application to control grass and some broadleaf weeds. They target microtubules to arrest cell division and inhibit the development of roots and shoots. Effects of a pendimethalin‐based herbicide were assessed on the gut microbial community of Pterostichus melas italicus Dejean, 1828 (Coleoptera, Carabidae). The exposure effect was tested in vivo by using a recommended field rate (4 L per ha, 330 gL−1 of active ingredient) and evaluating the variability of responses in 21 days, corresponding to the half‐life of pendimethalin. The 16S rRNA sequencing data showed that the gut lumen was dominated by Proteobacteria, Firmicutes, Fusobacteria, Tenericutes and Bacteroidetes. The exposure interfered with the bacterial community richness and diversity associated with the gut from 2 days after the treatment. The differential abundance analyses highlighted a shift involving Lactobacillaceae, Streptomycetaceae, Neisseriaceae, Ruminococcaceae and Enterobacteriaceae. An increase in species such as Enterobacter sp., Pseudomonas sp., Pantoea sp and Paracoccus sp. involved in the herbicide degradation was also recorded after 21 days of exposure. Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analysis indicated that the exposure has effects on the most predicted functional categories of gut microbiota related to metabolic function including carbohydrate, amino acid and lipid metabolism. These results demonstrate that pendimethalin can impact microbial communities associated with generalist predators inhabiting croplands leading to severe implications for the species’ ecological role. Understanding the effects of herbicides such as pendimethalin on ground beetles may help to protect beneficial soil insects that have a crucial role in the ecosystem services.

show abstract

Section: Discussionmentioning

confidence: 99%

“…fontium, a free‐living bacterium isolated from an environment that under anaerobic conditions utilizes monosaccharides and their derivatives but lacks fatty acid degradation pathways (Snopková et al. 2017). The genus Budvicia has been identified in the red palm weevil (Tagliavia et al.…”

Section: Discussionmentioning

confidence: 99%

Gut microbial community response to herbicide exposure in a ground beetle

Giglio

Vommaro

Gionechetti

et al. 2021

J Applied Entomology

View full text Add to dashboard Cite

show abstract

“…H 2 S mainly results from the fermentation of undigested sulphur protein in the hindgut, as well as the reduction of oxidized inorganic sulphur compounds, such as sulphate radicals (SO 4 2− ), by sulphur-reducing bacteria (SRB) [ 17 , 19 ]. There are many bacterial genera involved in sulphate reduction or protein degradation, such as Desulfovibrio , Veillonella , Megasphaera and Enterobacteria [ 20 , 21 , 22 ]. A previous study indicated that, under similar environments, host species have the potential to affect the composition of intestinal microorganisms, including some gas-producing ones [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Laying Hen Species on Odour Emissions

et al. 2020

Animals

View full text Add to dashboard Cite

Odour is one of the main environmental concerns in the laying hen industry and may also influence animal health and production performance. Previous studies showed that odours from the laying hen body are primarily produced from the microbial fermentation (breakdown) of organic materials in the caecum, and different laying hen species may have different odour production potentials. This study was conducted to evaluate the emissions of two primary odorous gases, ammonia (NH3) and hydrogen sulphide (H2S), from six different laying hen species (Hyline, Lohmann, Nongda, Jingfen, Xinghua and Zhusi). An in vitro fermentation technique was adopted in this study, which has been reported to be an appropriate method for simulating gas production from the microbial fermentation of organic materials in the caecum. The results of this study show that Jingfen produced the greatest volume of gas after 12 h of fermentation (p < 0.05). Hyline had the highest, while Lohmann had the lowest, total NH3 emissions (p < 0.05). The total H2S emissions of Zhusi and Hyline were higher than those of Lohmann, Jingfen and Xinghua (p < 0.05), while Xinghua exhibited the lowest total H2S emissions (p < 0.05). Of the six laying hen species, Xinghua was identified as the best species because it produced the lowest total amount of NH3 + H2S (39.94 µg). The results for the biochemical indicators showed that the concentration of volatile fatty acids (VFAs) from Zhusi was higher than that for the other five species, while the pH in Zhusi was lower (p < 0.01), and the concentrations of ammonium nitrogen (NH4+), uric acid and urea in Xinghua were lower than those in the other species (p < 0.01). Hyline had the highest change in SO42− concentration during the fermentation processes (p < 0.05). In addition, the results of the correlation analysis suggested that NH3 emission is positively related to urease activities but is not significantly related to the ureC gene number. Furthermore, H2S emission was observed to be significantly related to the reduction of SO42− but showed no connection with the aprA gene number. Overall, our findings provide a reference for future feeding programmes attempting to reduce odour pollution in the laying hen industry.

show abstract

Diversity of the aerobic cloacal microbiota of syntopic lizard species (Reptilia: Sauria) from a low-mountain area in Western Bulgaria

Lazarkevich,

Engibarov,

Mitova

et al. 2024

Ecol. Monten.

View full text Add to dashboard Cite

Compared to other reptile groups in Europe, lizards have generally been neglected and understudied in terms of microbiota research. In this study, we aimed to isolate, identify and characterize the aerobic cloacal microflora of wild-dwelling lizard hosts. We examined a total of 86 individuals from five species belonging to three families: the European green lizard (Lacerta viridis), the common wall lizard (Podarcis muralis), the meadow lizard (Darevskia praticola) (Lacertidae), the European snake-eyed skink (Ablepharus kitaibelii) (Scincidae) and the European slow worm (Anguis fragilis) (Anguidae) which co-occur in a low-mountain region in Western Bulgaria. In general, a similar composition of the resident microbial communities in the cloaca was found, accompanied by variation in the relative abundance of some bacterial taxa between the lizard species. A variety of Gram-negative and Gram-positive bacteria was isolated from the cloacal samples. Some of these bacteria are also known as opportunistic pathogens, both for hosts and humans. The bacterial species Hafnia alvei, Pseudomonas aeruginosa, Klebsiella oxytoca and representatives of Enterobacter spp., Citrobacter spp. and Enterococcus spp. were among the most prevalent.

show abstract

Free-Living Enterobacterium Pragia fontium 24613: Complete Genome Sequence and Metabolic Profiling

Cited by 3 publications

References 45 publications

Gut microbial community response to herbicide exposure in a ground beetle

Gut microbial community response to herbicide exposure in a ground beetle

Effects of Different Laying Hen Species on Odour Emissions

Diversity of the aerobic cloacal microbiota of syntopic lizard species (Reptilia: Sauria) from a low-mountain area in Western Bulgaria

Contact Info

Product

Resources

About