Free polyamine concentrations in coastal seawater during phytoplankton bloom

Nishibori, Naoyoshi; Yuasa, Akihiko; Sakai, Motosuke; Fujihara, Shinsuke; Nishio, S.

doi:10.1046/j.1444-2906.2001.00202.x

Cited by 36 publications

(36 citation statements)

References 16 publications

(18 reference statements)

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“…Typical components of eukaryotic cytosols can be transported by the roseobacters, such as polyamines, taurine, phosphoesters, phosphonates, aromatic metabolites, glyoxylate, allophanate, acetate, glycine betaine, DMSP, branched-chain amino acids, and organic acids. Many of these compounds are produced by phytoplankton or zooplankton (23,30,34,44) and may become available to roseobacters through exudation, diffusion-driven loss, or trophodynamic interactions including grazing and viral lysis. Regular observations of associations between roseobacters and marine phytoplankton in algal blooms (45,49) and phycospheres of cultured dinoflagellates and diatoms (1,25) are consistent with this idea.…”

Section: Vol 73 2007 Ecological Genomics Of Marine Roseobacters 4567mentioning

confidence: 99%

Ecological Genomics of Marine Roseobacters

Moran

Belas

Schell

et al. 2007

Appl Environ Microbiol

342

299

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Bacterioplankton of the marine Roseobacter clade have genomes that reflect a dynamic environment and diverse interactions with marine plankton. Comparative genome sequence analysis of three cultured representatives suggests that cellular requirements for nitrogen are largely provided by regenerated ammonium and organic compounds (polyamines, allophanate, and urea), while typical sources of carbon include amino acids, glyoxylate, and aromatic metabolites. An unexpectedly large number of genes are predicted to encode proteins involved in the production, degradation, and efflux of toxins and metabolites. A mechanism likely involved in cell-to-cell DNA or protein transfer was also discovered: vir-related genes encoding a type IV secretion system typical of bacterial pathogens. These suggest a potential for interacting with neighboring cells and impacting the routing of organic matter into the microbial loop. Genes shared among the three roseobacters and also common in nine draft Roseobacter genomes include those for carbon monoxide oxidation, dimethylsulfoniopropionate demethylation, and aromatic compound degradation. Genes shared with other cultured marine bacteria include those for utilizing sodium gradients, transport and metabolism of sulfate, and osmoregulation.In surface waters of the open ocean, 1 in 10 bacterial cells is a member of the Roseobacter group (17). In coastal waters, the number of Roseobacter cells increases to 1 in 5 (11, 19). Despite their obvious ecological success, however, roseobacters do not fit the stereotype of a small, metabolically conservative, "oligotrophic" bacterium (8, 18). Instead, they are large (0.08 m 3 ) (38), easily cultured (19), and respond readily to increased substrate availability (7). Analysis of the first Roseobacter genome sequence, that of Silicibacter pomeroyi, revealed a fairly large genome (4.5 Mb) housing abundant and diverse transporters, complex regulatory systems, and multiple pathways for acquiring carbon and energy in seawater. Roseobacters thus appear to be quite versatile from metabolic and ecological standpoints (43), with an assortment of strategies for obtaining carbon and nutrients and, directly or indirectly, affecting the biogeochemical status of seawater.The availability of two additional closed genome sequences of cultured roseobacters provides the opportunity for an ecologically based analysis of the genetic capabilities of this bacterial taxon. The three organisms are assumed to have different niches in the surface ocean based on the conditions of their isolation: S. pomeroyi is a free-living heterotrophic bacterioplankter obtained from coastal seawater (43), congener Silicibacter sp. strain TM1040 (96% 16S rRNA sequence identity to S. pomeroyi [ Fig. 1]) is an associate of the marine dinoflagellate Pfiesteria piscicida (1, 40), and Jannaschia sp. strain CCS1 (with 94% 16S rRNA sequence identity to the two Silicibacter species) represents a recently discovered class of marine aerobic bacteriochlorophyll a-based phototrophs (4). Our comparative an...

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Section: Vol 73 2007 Ecological Genomics Of Marine Roseobacters 4567mentioning

confidence: 99%

Ecological Genomics of Marine Roseobacters

Moran

Belas

Schell

et al. 2007

Appl Environ Microbiol

342

299

View full text Add to dashboard Cite

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“…However, they are suspected to encode proteins involved in the degradation of polyamines (19), a class of nitrogen-rich compounds including putrescine and spermidine that form complexes with DNA and RNA and act as important signaling compounds for cell growth (20). Evidence in support of a hypothesis that the aphA transcripts reflect a role for polyamines as a nitrogen source for coastal bacterioplankton includes the facts that polyamines are produced by marine algae, plants, invertebrates, and microorganisms (8,13,16,20), they reach concentrations of 30 nM in coastal seawater during algal blooms (16), and they are readily assimilated by coastal and open ocean bacterioplankton communities (8). Further, the genome sequence of marine bacterium S. pomeroyi contains an aphA homolog located in an apparent operon with a polyamine transporter (potABCD) (15) and candidate genes for a putrescine degradation pathway (putrescine transaminase and aminobutyraldehyde dehydrogenase).…”

mentioning

confidence: 99%

Analysis of Microbial Gene Transcripts in Environmental Samples

Poretsky

Bano

Buchan

et al. 2005

Appl Environ Microbiol

210

135

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We analyzed gene expression in marine and freshwater bacterioplankton communities by the direct retrieval and analysis of microbial transcripts. Environmental mRNA, obtained from total RNA by subtractive hybridization of rRNA, was reverse transcribed, amplified with random primers, and cloned. Approximately 400 clones were analyzed, of which ϳ80% were unambiguously mRNA derived. mRNAs appeared to be from diverse taxonomic groups, including both Bacteria (mainly ␣-and ␥-Proteobacteria) and Archaea (mainly Euryarchaeota). Many transcripts could be linked to environmentally important processes such as sulfur oxidation (soxA), assimilation of C1 compounds (fdh1B), and acquisition of nitrogen via polyamine degradation (aphA). Environmental transcriptomics is a means of exploring functional gene expression within natural microbial communities without bias toward known sequences, and provides a new approach for obtaining communityspecific variants of key functional genes.

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“…However, they both react equally with primary polyamines, biogenic amines and amino sugars (van Eijk et al 1996;Appuhn et al 2004;Lozanov et al 2007;Brückner et al 2012). All these compounds may be found in marine water and particulate material (Antia et al 1991;Yang et al 1993;Nishibori et al 2001;Müller et al 2009). Thus, these compounds may give extraneous peaks during chromatographic analyses.…”

Section: Devez and Delmasmentioning

confidence: 99%

Selective liquid chromatographic determination of trace domoic acid in seawater and phytoplankton: improvement using the o‐phthaldialdehyde/9‐fluorenylmethylchloroformate derivatization

Devez

Delmas

2013

Limnology & Ocean Methods

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Domoic acid (DA), the amnesic shellfish poisoning toxin (ASP), is an excitatory amino acid that can accumulate, under certain environmental conditions, in shellfish, finfish, birds, and mammals, by direct filtration or by feeding on contaminated organisms (Mos 2001). This potent neurotoxin with three carboxylic groups, responsible for its high polarity and hydrophilicity, is a cyclic amino acid and a secondary amine with a molecular weight of 311 Daltons. DA belongs to a neurotransmitter class of compounds with a structure very similar to an important neurotransmitter excitatory, acid glutamate, and indeed mimics glutamate in its interaction with some of its receptor subtypes. DA can damage the neurons by activating R-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and kainate receptors, causing an influx of calcium, and then be the cause of several symptoms such as nausea, disorientation, temporary amnesia and, in more serious cases, persistent memory loss and/or coma, and ultimately even death (Costa et al. 2010). This toxin was originally isolated from a red microalga Chondria armata by Japanese researchers (Takemoto and Daigo 1958) and is now reported to be produced in several species of marine diatoms from the genus Pseudo-nitzschia. DA was identified as the causative agent of the well-known tragic intoxication event observed in 1987 (Prince Edward Island from Cardigan Bay in Eastern Canada). More than one hundred people became ill and three victims died, by septic shock or pneumonia, 11-24 days after intoxication. Whereas most victims recovered within 10 days, after a period of confusion and nausea, the others continued to show signs of selective short-term memory loss (Bates et al. 1989). Additional consequences of the DA presence included the temporary closure of shellfish aquaculture industries and a strong impact on tourism activities (Pistocchi et al. 2012;Trainer et al. 2012).The detection limit of the method generally considered as the reference for particulate DA quantification in marine organisms (high-performance liquid chromatography with ultra-violet detection at λ = 242 nm, HPLC-UV) ranges between 13 and 250 nM depending on the detector (Quilliam 2003). This sensitivity threshold is often insufficient to follow the dynamics of both dissolved and particulate domoic acid AbstractDomoic acid (DA), a toxin produced worldwide by some species of the genus Pseudo-nitzschia, is responsible for contamination of marine molluscs, mammals, birds, and for human intoxication, and when detected in high levels results in closures of shellfish farms, thus causing severe economic losses to aquaculture. Studies on algal production of DA in cultures and field samples require sensitive methods capable of measuring trace concentrations of domoic acid. Measuring domoic acid concentrations at trace levels is still a significant challenge. A sensitive and reliable double derivatization using o-phthaldialdehyde-mercaptoethanol/9-fluorenylmethylchloroformate (OPA-MeSH/FMOC-Cl) followed by high-performance liq...

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Free polyamine concentrations in coastal seawater during phytoplankton bloom

Cited by 36 publications

References 16 publications

Ecological Genomics of Marine Roseobacters

Ecological Genomics of Marine Roseobacters

Analysis of Microbial Gene Transcripts in Environmental Samples

Selective liquid chromatographic determination of trace domoic acid in seawater and phytoplankton: improvement using the o‐phthaldialdehyde/9‐fluorenylmethylchloroformate derivatization

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