Free-radial oxidation of lipids in human serum as a function of vitamin E content

Abstract

The relationship between free-radial oxidation of human serum lipids and serum content of vitamin E is studied by the chemiluminescence method. A linearity between chemiluminescence and vitamin E content is established. By approximating experhnental data we deduced a set of equations characterizing the chemiluminescence parameters as a function of vitamin E content. The correlation coefficients have been calculated in the 0:7605-0.9671 range.Key Words: chemiluminescence; vitamin E; free radicals; antioxidants; lipid peroxidation Free-radical oxidation (FRO) of lipids has been extensively studied. It is shown that both in vivo [1,12] and in vitro [3,13] FRO modifies physicochemical properties of cell membranes and, consequently, impairs their functions, leading to the development of various pathologies [8,13].Previously, we evaluated the role of synthetic and naturally occurring antioxidants (AO) in FRO of individual lipids and blood lipids in vitro [3] as well as the role of vitamin E in pathological processes [6]. However, there is no information regarding the relationship between the intensity of FRO of serum lipids and the content of vitamin E, the major fatsoluble serum AO [11], in healthy subjects.Our goal was to study the kinetics of FRO of serum lipids as a function of vitamin E content in healthy men using the chemiluminescence (CL) method. MATERIALS AND METHODSSera from 24 healthy male donors aged 28-48 years were used. Blood was collected from the cubital vein [5], ratio of Im~ • to the time, during which CL intensity decreases 2-fold (T) [7], and the tangent of the descending segment of the kinetic curve (tgc 0.Chemiluminescence was measured in a PKhL-01 device equipped with a FEU 84-3 photomultiplying unit and calibrated in absolute units quantum/ secX47t. Kinetic curves were recorded and processed with an AkhLG-2-01 analyzer using specially designed software. Serum concentration of vitanain E was determined as described elsewhere [10].Vitamin E concentration and CL parameters were measured randomly. In each experiment, the mean of three determinations was calculated. Correlations were calculated by approximation using the least squares method. RESULTSThe kinetics of serum CL recorded in the presence of hydrogen peroxide agrees with the literature data [5,8]. The fast flash of CL reaches the lnaximum that

Serum total oxidant/anti-oxidant status, ischemia-modified albumin and oxidized-low density lipoprotein levels in patients with vitamin D deficiency

“…investigated the effects of vitamin D on oxidative stress and lipid peroxidation in animals by determining the changes in lipid peroxidation before and after the replacement in vitamin D-deficient animals (23). They demonstrated that high levels of thiobarbituric acid reactive substances (TBARS) indicating that lipid peroxidation in vitamin D deficient animals were decreased significantly after vitamin D3 replacement, but still remained higher than controls (24,25). Tarcin and cols.…”