Free-radical crosslinking copolymerization of acrylamide and N,N′-methylenebis acrylamide by used Ce(IV)/polyethylene glycol and Ce(IV)/diethylmalonate redox initiator systems

“…Additional methods of altering the gel pore structure not considered here include adjusting the amount of cross-linker present in the gel mix [14,15] and varying the photo-initiator concentration in order to modify the polymerization kinetics [13]. These modifications may provide more favorable sieving performance and higher separation resolution.…”

Section: Discussionmentioning

confidence: 99%

“…4b), however, the change is more pronounced, especially at the lowest exposure level. This trend is likely to be a manifestation of the complex interplay between reaction kinetics gel pore morphology [13][14][15]. It is notable that despite our limited ability to vary the incident UV intensity we were still able to induce nontrivial changes in the diffusion and dispersion characteristics of the gels.…”

Section: Influence Of Polymerization Kineticsmentioning

confidence: 94%

Microdevice-based measurements of diffusion and dispersion in cross-linked and linear polyacrylamide DNA sequencing gels

Ugaz¹,

Burke

Burns³

2002

Electrophoresis

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We use microfabricated gel electrophoresis devices incorporating integrated on-chip electrodes, heaters, and temperature sensors to measure diffusion and dispersion of single-stranded DNA fragments in cross-linked and uncross-linked polyacrylamide gels. The microdevice format allows a complete set of diffusion and dispersion data to be collected in approximately one hour. These results are compared with corresponding data obtained in a macroscale DNA sequencer, and the effects of gel composition and initiation chemistry are explored. Although the diffusion and dispersion data exhibit similar qualitative trends both on chip and on the macroscale, the magnitudes of the coefficients measured in the microdevice are somewhat higher. This discrepancy is likely due to altered polymerization kinetics arising as a consequence of using a UV-initiated polymerization chemistry to cast the on-chip gels as opposed to the standard chemical polymerization employed on the macroscale. We also find that reductions in the magnitudes of diffusion and dispersion coefficients are achieved at higher polymer concentrations and at operating temperatures in the vicinity of 50 degrees C. Finally, we find that cross-linked polyacrylamide gels yield significantly lower diffusion and dispersion coefficients than linear polyacrylamide. These findings can be used to identify rational strategies to improve separation performance in both micro- and macroscale gel electrophoresis systems.

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“…As a result they attract increasing interest in various application areas such as drug delivery systems, separation operations in biotechnology, processing of agricultural products, conductive or superabsorbent composites, sensors and actuators [1][2][3][4][5][6][7][8][9][10][11][12]. The swelling equilibrium of gels is determined by both the free energy of elasticity and free energy of mixing.…”

Section: Introductionmentioning

confidence: 99%

Swelling properties of acrylamide-N,N’-methylene bis(acrylamide) hydrogels synthesized by using meso-2,3-dimercaptosuccinic acid-cerium(IV) redox couple

Özeroğlu¹,

Birdal²

2009

Express Polym. Lett.

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Abstract. In this paper, meso-2,3-dimercaptosuccinic acid-Ce(IV) redox couple is used for crosslinking polymerization of acrylamide (AAm) with N,N′-methylene bis(acrylamide) (MBAA) in acid aqueous medium. We have investigated the effects of crosslinker ratio, acid concentration and the molar ratio of AAm/Ce(IV) on the swelling behaviors of the synthesized P(AAm-MBAA) hydrogels. The experimental results show that the increase in the nAAm/nMBAA and nAAm/nCe(IV) result in an increase in the swelling ratio and rate of resulting hydrogels. However, the swelling equilibrium ratio and rate have been decreased with increasing of acid concentration. The k and n values in the equation of swelling rate have been determined. The n exponent decreases with increasing crosslinker, initiator and acid concentrations whereas k values increase. Kinetics and equilibrium swelling values have been discussed in terms of reaction parameters.

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Free-radical crosslinking copolymerization of acrylamide and N,N′-methylenebis acrylamide by used Ce(IV)/polyethylene glycol and Ce(IV)/diethylmalonate redox initiator systems

Cited by 18 publications

References 21 publications

Basic Features and Techniques

Basic Features and Techniques

Microdevice-based measurements of diffusion and dispersion in cross-linked and linear polyacrylamide DNA sequencing gels

Swelling properties of acrylamide-N,N’-methylene bis(acrylamide) hydrogels synthesized by using meso-2,3-dimercaptosuccinic acid-cerium(IV) redox couple

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