Free radical oxidation and antioxidant activity in placental tissue in preterm labor

Prokopenko, V. M.; Arutyunyan, A. V.; Frolova, Elena Vladimirovna; Kuzminykh, Tatyana U.; Ailamazyan, Eduard К.

doi:10.1007/bf02445114

Cited by 2 publications

(2 citation statements)

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“…The generation of reactive oxygen species (ROS) was studied in brain and liver homogenates and in serum according to a peroxide-luminol-dependent chemiluminescence method [36] on a Emilite EL-1105 chemiluminometer (BioChimMac, Moscow, Russia). The intensity of the peroxide lipid oxidation was estimated on the basis of the diene conjugate and Schiff base contents [37].…”

Section: Biochemical Assaysmentioning

confidence: 99%

Effects of Phentermine and Phenformin on Biomarkers of Aging in Rats

et al. 2004

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Background: Caloric restriction (CR) is the only treatment known to substantially prolong both average and maximal life span in experimental animals. Interventions that mimic certain effects of CR could be potential anti-aging treatments in humans. Drugs which reduce appetite (anorexiants) represent one class of candidate treatments. Agents that reduce the glucose utilization by the organism could also represent another class of candidate CR mimetics. Objective: In our study, we addressed the following questions: (1) Does treatment with an anorexiant reduce caloric intake and body weight of experimental animals comparable to that caused by CR? (2) Does treatment with an antidiabetic agent influence caloric intake and body weight? (3) Does treatment with any of these drugs affect metabolic parameters of an organism in the way similar to that observed with CR? Methods: One hundred and twenty 6-month-old female Wistar-derived LIO rats were randomly subdivided into four groups and exposed to: (1) ad libitum feeding with placebo (controls); (2) the antidiabetic drug phenformin (2 mg/kg); (3) the anorectic drug phentermine (1 mg/kg), and (4) the same amount of food as the group with the least food intake during the previous week (pair-fed controls). Food and water intake, body weight, and rectal temperature were measured weekly during 16 weeks. At the end of the 16th week of the experiment, serum levels of glucose, total β-lipoprotein and pre-β-lipoprotein fractions, cholesterol, triglycerides, insulin, total triiodothyronine, and free thyroxine were estimated. The contents of diene conjugates and Schiff’s bases, total antioxidant activity, the activities of Cu/Zn superoxide dismutase, glutathione S-transferase, and glutathione peroxidase, and the generation of reactive oxygen species (ROS) were studied in brain and liver homogenates and in the serum. Results: The controls exposed to pair feeding had a significantly reduced food consumption (about 20%) as compared with the ad libitum fed controls and thus exhibited a moderate CR. Treatment with phentermine reduced the caloric intake by about 12% as compared with the ad libitum fed controls. Body weight and water intake in this group were only slightly decreased (by about 2 and 5%, respectively) as compared with the controls. The mean rectal temperature in the phentermine group (38°C) was significantly higher than in the ad libitum fed (37.8°C) and pair-fed (37.6°C) controls. Treatment with phentermine also resulted in significantly reduced ROS levels in all tissues studied, while the highest ROS production was found in ad libitum (blood serum) and pair-fed (brain) controls. Treatment with phenformin did not significantly influence food and water consumption, body weight, and temperature when compared with the ad libitum fed controls. Rats treated with this antidiabetic drug showed intermediate values of ROS generation. Differences among the groups in total antioxidant activity were not obvious. Conclusions: Treatment with phentermine reduces caloric intake slightly le...

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Section: Biochemical Assaysmentioning

confidence: 99%

Effects of Phentermine and Phenformin on Biomarkers of Aging in Rats

et al. 2004

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“…Chronic placental insufficiency is observed in about 30% women at a high risk of spontaneous abortion. Lipid peroxidation (LPO) causes membrane insufficiency and abnormalities, including placental insufficiency [6,8]. In pathological pregnancy, e. g., in gestosis, the disease severity correlates with LPO intensification [3].…”

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confidence: 99%